Monitor The Run - abi 7500 Getting Started Manual

Chapter 4 Run the Relative Standard Curve Experiment

Monitor the Run

Monitor the Run

Notes
72
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
You can view the progress of the run in real time as described below. During the run,
periodically view all three available plots from the 7500 software for potential problems.
To...
Stop the run
View amplification data
in real time
View progress of the
run in the Run Method
screen
Enable/disable the
Notification Settings
About the Amplification Plot Screen
This screen displays sample amplification as your instrument collects fluorescence data
during a run. If a method is set up to collect real-time data, the Amplification Plot screen
displays the data for the wells selected in the View Plate Layout tab. The plot displays
normalized dye fluorescence (∆Rn) as a function of cycle number. The figure below
shows the Amplification Plot screen as it appears during the example experiment.
To view data in the Amplification Plot screen, select the wells that you want to view in
the View Plate Layout tab.
1. In the 7500 software, click STOP RUN.
2. In the Stop Run dialog box, click one of the following:
– Stop Immediately to stop the run immediately.
– Stop after Current Cycle/Hold to stop the run after the
current cycle or hold.
– Cancel to continue the run.
Select Amplification Plot.
See "About the Amplification Plot Screen" on page 72.
Select Run Method.
See "About the Run Method Screen" on page 73.
Select or deselect Enable Notifications.
See "Enable the Notification Settings (Optional)" on page 69.
Action
and Comparative C
T
Experiments