Prepare The Reaction Plate - abi 7500 Getting Started Manual

Chapter 7 Prepare the Comparative C

Prepare the Reaction Plate

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Information

Prepare the Reaction Plate

About the
Example
Experiment
Required
Materials
Notes
146
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
Reactions
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For more information on preparing the reaction mix, refer to the protocol appropriate for
the reagents you are using in the PCR reactions:
®
• TaqMan Gene Expression Assays Protocol
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• Custom TaqMan Gene Expression Assays Protocol
Prepare the reactions for each replicate group, then transfer them to the reaction plate.
Use the plate layout displayed in the 7500 software.
For the comparative C example experiment:
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®
• A MicroAmp Optical 96-Well Reaction Plate is used.
• The reaction volume is 50 µL/well.
• The reaction plate contains:
– 96 Unknown wells
– 0 Negative Control wells
– 0 Empty wells
• The plate layout that is automatically generated by the 7500 software is used:
• Microcentrifuge tubes
• Pipettors
• Pipette tips
• Reaction mixes (from page 145)
• Water
• Samples (from page 143)
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• MicroAmp Optical 96-Well Reaction Plate
™
• MicroAmp Optical Adhesive Film
• Centrifuge
and Comparative C Experiments
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