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Numerics
,
1-step RT-PCR 10
,
2-step RT-PCR 10
7500/7500 Fast system
consumables 4
data collection 2
,
,
filters 3
110
186
reagents 11
A
Advanced Setup 12
204
alternate experiment workflows. See workflows.
amplification efficiency 30
Amplification Plot screen
monitor during a run 72
view after a run 85
amplification plot, typical 90
,
AMPNC flag 104
analysis screens
Amplification Plot screen 85
Compare Analysis Settings screen 202
Gene Expression Plot screen 93
Multicomponent Plot screen 107
Multiple Plots screen 81
navigation tips 80
QC Summary screen 103
Raw Data Plot screen 109
Standard Curve screen 82
,
Well Table 93
analysis settings
,
advanced 101
177
,
baseline 101
177
compare 202
,
CT 101
177
,
flag 101
177
relative quantitation 101
,
threshold 101
177
,
,
view 100
176
analyze experiment
,
analyze 78
158
for more information 84
,
110
165
,
guidelines 80
84
,
159
165
,
omit wells 105
publish the data 174
view Amplification Plot screen 85
view analysis settings 100
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
Experiments
T
,
,
,
,
,
,
25
26
36
42
118
126
,
,
,
,
,
14
15
25
26
117
118
,
,
,
,
,
,
,
13
25
33
63
117
118
123
,
,
,
31
84
178
,
,
166
200
,
171
180
,
,
166
200
,
,
162
196
,
,
182
200
,
,
161
201
,
160
,
,
179
201
,
185
162
,
177
202
,
,
,
,
,
92
96
102
104
106
,
,
,
,
,
173
178
180
182
184
,
,
,
,
,
,
90
96
101
104
106
109
,
,
,
,
,
171
177
180
182
184
181
,
166
,
176
view Gene Expression Plot screen 93
view Multicomponent Plot screen 107
130
view Multiple Plots screen 81
view QC Summary screen 103
view Raw Data Plot screen 109
view Standard Curve screen 82
view Well Table 93
workflow 76
analyze study
analyze 195
for more information 192
guidelines 199
,
,
148
omit wells 199
view Amplification Plot screen 200
view analysis settings 202
view Compare Analysis Settings screen 202
view Experiment Data 195
view Gene Expression Plot screen 196
view Multicomponent Plot screen 200
view Multiple Plots screen 201
view QC Summary 201
Applied Biosystems
contacting xi
customer feedback on documentation xi
Technical Support xi
Applied Biosystems 7500/7500 Fast Real-Time PCR
System. See 7500/7500 Fast system.
assumptions for using this guide viii
B
BADROX flag 104
baseline
correct values 90
examples 92
manually adjust 101
biohazardous waste, handling xix
biological hazard guidelines xxi
biological replicate groups
add to experiment 32
add to study 192
BLFAIL flag 104
,
,
109
C
,
186
,
,
110
CAUTION, description xii
,
186
chemical safety xvii
chemical waste safety xix
column
C
Mean 95
T
Index
,
162
,
,
161
,
179
,
185
,
162
,
199
,
200
,
180
,
171
,
173
,
177
,
,
33
122
,
180
,
xviii
,
,
164
198
182
229
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