Omit Wells From The Analysis - abi 7500 Getting Started Manual

Omit Wells from the Analysis

Experimental error may cause some wells to be amplified insufficiently or not at all.
These wells typically produce C
associated replicate wells. If included in the calculations, these outliers can result in
erroneous measurements; to ensure precision, omit the outliers from the analysis.
About the In the example experiment, you use the Well Table to remove well A9 from the analysis.
Example
The C
Experiment which generated the HIGHSD flag due to the degree of variation in their C
"View the QC Summary" on page 103).
Omit Wells 1. In the navigation pane, select Analysis
2. In the Plot Type drop-down list, select C
3. Select the View Well Table tab.
4. In the Well Table:
2
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
T
Chapter 5 Analyze the Relative Standard Curve Experiment
values that differ significantly from the average for the
T
of well A9 deviates significantly from those of the related technical replicates,
T
If no data are displayed, click Analyze.
Note:
In the Group By drop-down list, select Replicate.
a.
Look for any outliers in the replicate group (be sure they are flagged). In the
b.
example experiment, well A9 is the outlier.
Select the Omit check box next to well A9.
c.

Omit Wells from the Analysis

Amplification Plot.
vs Well.
T
values (see
T
3
4a
4c
105