Define The Methods And Materials - abi 7500 Getting Started Manual

Chapter 6 Design the Comparative C

Define the Methods and Materials

Define the Methods and Materials

About the
Example
Experiment
Complete the
Methods and
Materials Screen
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Notes
116
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
Experiment
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In the Methods and Materials screen, select the quantitation method, reagents, ramp
speed, and PCR template to use for the experiment.
In the comparative C example experiment:
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• The comparative C
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®
• TaqMan reagents are used.
• The standard ramp speed is used in the instrument run.
• cDNA (prepared from total RNA isolated from liver, kidney, brain, and universal
tissues) is the template type. You must first perform reverse transcription to convert
the RNA to cDNA (see "Prepare the Template" on page 139).
1. Select Comparative C
®
2. Select TaqMan Reagents for the reagents. (default)
IMPORTANT! Fast runs cannot be performed on a 7500 system.
3. Select Standard (~ 2 hours to complete a run) for the ramp speed.
4. Select cDNA (complementary DNA) for the template type. (default)
5. Click Next.
quantitation method is used.
(∆∆C ) as the quantitation method.
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and Comparative C Experiments
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