Contact With Liquids - Leica TCS SP8 STED 3X User Manual

• Do not introduce any reflective objects or mirrors into the laser beam path or specimen
area when working on a laser.
• Never expose your eyes or skin to direct or indirect laser radiation (reflections). The
radiation can cause irreparable eye and skin injuries.
• Do not look into the eyepieces when scanning or switching the beam path in the
microscope.
• During the scanning operation, the laser radiation is accessible in the microscope's
specimen area without obstruction after coming out of the objective. Always maintain a
nominal ocular hazard distance of at least 20 cm (8") between your eyes and the opening
of the objective.
Figure 1:
• Only use S1 and S28 Leica microscope condensers.
• Do not use an S70 microscope condenser. Its large working distance and lower
numerical aperture could pose a hazard due to laser radiation.
• Never change any samples, objectives, filter cubes, beam splitters, condensers or other
components during a scanning operation.
• If there is no lamp housing or mirror housing connected to the microscope, attach the
corresponding cover to the replacement flange.
• Close all unoccupied positions in the objective turret using the supplied caps.
Special features for MP systems:
• When using a MP system, you must wear suitable laser safety goggles. Appropriate
laser safety goggles for IR laser radiation are provided with the system when delivered.
These laser safety goggles do not provide any protection against visible laser radiation
(visible spectrum)! During the scanning operation, all persons present in the room must
wear such laser safety goggles. If you need additional safety goggles, please contact the
Leica branch office in your country directly.
• Do not use any dry objectives with a numerical aperture (NA) greater than 0.85. This does
not apply to immersion objectives (oil, water).
5.5

Contact with liquids

• Do not expose the system to rain or humidity. Otherwise, risks such as electrical shocks
Specimen area of upright and inverted microscope
General Safety Notes Revision
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