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10.1 Cleaning
• Remove frozen section waste from the cold cry-
ostat.
• Remove the waste tray and empty it.
• Remove the storage shelves and brush shelf for
cleaning.
• Switch off the cryochamber illumination and wait
until the lamp has reached room temperature.
Do not use organic solvents or any other ag-
gressive substances for cleaning and disin-
fection!
We strongly recommend the use of Leica
Cryofect disinfectant spray!
Only use alcohol or common disinfectants
based on alcohol!
Leica CM1510 – Cryostat
10. Cleaning and disinfection
10.2 Spray disinfection with Leica Cryofect
Keep only in original container and up to
25 °C!
Highly flammable (VbF: B)!
Flash-point: 18 °C (DIN 51755)
Keep away from sources of ignition!
Do not spray into flames!
Attention with alcohol-sensitive surfaces!
Wear appropriate protective clothing when
cleaning and disinfecting!
Disinfect the instrument and accessories ev-
ery day!
Effectiveness of the disinfectant down to -20
°C experimentally tested.
1. Select a cryochamber temperature value down to -
20 °C.
2. Remove the knife or blade from the knife holder.
3. Remove all samples, microscope slides and tools
from the cryochamber.
4. Remove debris from the cryochamber.
Allow the cryochamber to reach the previously se-
lected temperature.
Once the selected temperature is reached, either
5a. spray the disinfectant evenly on the contaminated
surfaces- the surfaces should be covered with an
even layer - or
5b. soak a cloth with disinfectant and apply it on the
contaminated surfaces.
6. Allow a reaction time of no less than 15 minutes.
7. Wipe it off with a tissue.
8. Dispose of tissue in compliance with the ruling
waste disposal regulations of your institution.
9. Set the cryochamber temperature to the originally
selected value.
If increased frost buildup occurs, start a
manual defrost cycle.
• Place an appropriate vessel under the drain tube
on the right side of the cabinet.
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