Leica TCS SP8 STED 3X User Manual page 83

Tab area:
The displayed tabs depend on the selected step. In the TCS SP8's basic operating mode, the
following tabs are assigned to the steps:
• Acquire step: 
Project tab: Directory tree for opened files
Acquisition tab: Hardware settings for the current experiment and parameter settings
for image acquisition
• Process step: 
Project tab: Directory tree for opened files
Process Tools tab: Overview of the functions available in this step
• Quantify step: 
Project tab: Directory tree for opened files
Tools tab: Overview of the functions available in this step
Working area:
Depending on the step and tab selected, different dialogs are available in the working area:
• Acquire step: 
Beam Path Settings: In this dialog, the beam path is shown schematically. You can select
and adjust the lasers and the system components along the beam path and the detectors
for image acquisition.
• Process step: 
Appropriate processing options and a preview image appear in the working area based
on which function you have selected in the Process Tools tab.
• Quantify step: 
The working area is divided into two tabs:
Graphs: Graphic representation of values that were measured in the entire image or in
regions of interest (ROI) 
Statistics: Display of statistical values that were determined in the entire image or in
plotted regions of interest (ROI)
Display window:
Acquired images can be displayed and regions of interest (ROI) and additional information
can be plotted here.
Display window settings:
This area contains buttons used to change the display options for acquired images (such as
color).
Image processing and image analysis options:
This area contains buttons that can be used to add information to the image, such as text,
arrows or dimensions. In addition, there is a button that enables regions of interest (ROI) to
be plotted in the image display.
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