Table of Contents
Advertisement
3.3.2 Operation
The system software assumes the loop should only be 50% filled
for maximum compound recovery. If the parameters entered for
the individual injection exceed a 50% loop fill, a warning message
will appear recommending a change in the parameters. This
advice can be ignored at the discretion of the user.
The AutoSampler Module has an automatic probe wash built into
its operation. After each sample is complete, the probe goes to the
wash station. This station rinses the inner and outer surface of
the needle to minimize carryover. Air is then drawn into the
sample probe to remove the strong wash fluid. Place a suitable
wash fluid in a container, and place the lines from the wash
pump in this container.
Note
The wash fluid will build up contaminants over time due to the
rinsing of the sample probe and therefore should be changed
periodically.
To begin a separation sequence with the AutoSampler Module,
you will need to create a sample queue. After the AutoSampler
Module has been installed and the EZ Prep is in the P
mode, the software will display two tabs at the bottom of the
screen. Accessing the R
ation much like the standard EZ Prep (without an installed
AutoSampler Module). Pressing P
R
screen. On this screen, you can perform a manual
EQUIREMENTS
injection by removing the Luer fitting from the inject loop, or you
can perform multiple injections on a single sample from the
AutoSampler Module without creating a queue. Selecting the
Q
screen will allow you to set up a separation sequence.
UEUE
Note
If you are performing a manual injection, the Luer fitting or a
needle port must be reinstalled on the injection valve.
Follow the instructions below for further information on oper-
ating the AutoSampler Module:
1. Install a sample vial rack before opening the Q
This will allow the software to limit sample programming
to the positions available for sample vials.
2. Open the Q
UEUE
For an explanation of the available columns on this screen, see
Table 3-1.
Section 3 The AutoSampler Module
tab will result in a screen and oper-
UN
will display the R
LAY
tab.
Automation
HPLC
REP
UN
tab.
UEUE
3-21
Advertisement
Table of Contents
