Priming And Purging The System - Agilent Technologies 1200 Series Reference Manual

Priming and Purging the System

The system can be primed either by drawing solvent through the degasser with
a syringe or by pumping with the pump.
Priming the system with a syringe is recommended, when:
• vacuum degasser or connected tubings are used for the first time or vacuum
• changing to solvents that are immiscible with the solvent currently in the
Priming the system by using the pump at high flow rate
(3–5 ml/min) is recommended, when:
• pumping system was turned off for a length of time (for example, overnight)
• solvents have been changed.
When opening capillary or tube fittings solvents may leak out. Please observe
WA R N I N G
appropriate safety procedures (for example, goggles, safety gloves and protective
clothing) as described in the material handling and safety data sheet supplied by the
solvent vendor, especially when toxic or hazardous solvents are used.
Priming with a Syringe
Before using a new degasser or new tubings for the first time:
1 Prime all tubings with at least 30 ml of iso-propanol no matter whether the
1200 Series QP Reference Manual
tubes are empty or
vacuum tubes.
and if volatile solvent mixtures are used, or
channels will be used with organic mobile phase or with water.
Installing the Pump 1
27