Agilent Technologies 1200 Series Reference Manual page 28

1 Installing the Pump
If you are changing to a solvent that is immiscible with the solvent currently in
the tubing continue as follows:
2 Replace the current solvent with adequate organic solvent (see
3 Disconnect solvent outlet tube of the channel that is supposed to be primed
4 Connect syringe adapter to solvent outlet tube.
5 Push syringe adapter onto syringe.
6 Pull syringe plunger to draw at least 30 ml of solvent through degasser and
7 Replace the priming solvent with the new solvent of your choice.
8 Pull syringe plunger to draw at least 30 ml of solvent through degasser and
9 Disconnect syringe adapter from solvent tube.
10 Connect the solvent tube to the appropriate channel of the MCGV.
11 Repeat
When priming the vacuum degasser with a syringe the solvent is drawn through the
N O T E
degasser tubes very quickly. The solvent at the degasser outlet will therefore not be fully
degassed. Pump for approximately 10 minutes with your selected flow rate before starting
any application. This will allow the vacuum degasser to properly degas the solvent in the
degasser tubes.
The pump should never be used for priming empty tubings (never let the pump run dry). Use
N O T E
the syringe to draw enough solvent for completely filling the tubings to the pump inlet
before continueing to prime with the pump.
Priming with the Pump
When the pumping system has been turned off for a certain time (for example,
overnight) oxygen will rediffuse into the solvent channels between the vacuum
degasser and the pump. Solvents containing volatile ingredients will slightly
lose these, if left in the degasser without flow for a prolonged period of time.
Therefore priming of the vacuum degasser and the pumping system is
required before starting an application.
28
page 29), if current solvent is organic or with water, if current solvent is an
inorganic buffer or contains salt.
from your pump.
tubing.
tubing.
step 3 to step 10 for the other solvent channels.
Table 5
1200 Series QP Reference Manual
on