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Agilent Technologies 1200 Series Reference Manual

Vacuum degasser
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Agilent 1200 Series
Vacuum Degasser
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  • Page 1 Agilent 1200 Series Vacuum Degasser Reference Manual...
  • Page 2 (Computer Software) and, for Department of Defense purchases, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS 227.7202-3 (Rights in Commercial Computer Software or Computer Software Documentation). If a federal government or other public sector Customer has a need for 1200 Series VD Reference Manual...
  • Page 3 Instructions on how to repair the vacuum degasser Parts and Materials Detailed illustrations and listings for parts and materials identification Theory of Operation The vacuum degasser’s operation and electronics Specifications Performance specifications of the vacuum degasser Appendix Safety Information 1200 Series VD Reference Manual...
  • Page 4 1200 Series VD Reference Manual...

  • Page 5: Table Of Contents

    Normal Operation Mode 2 (Timing Mode) Continuous Mode Error Mode Operational Hints for the Vacuum Degasser Solvent Information Prevent Blocking of Solvent Filters Troubleshooting Status Indicators Hardware Symptoms All Lamps are Off and the Vacuum Degasser Appears Dead 1200 Series VD Reference Manual...
  • Page 6 Exchanging the Solenoid Valve Exchanging the Degasser Control Assembly and the Sensor Assembly Parts and Materials Vacuum Degasser Parts Cover Parts Sheet Metal Kit Power and Status Light Pipes Accessory Kit Cable Overview Remote Cables Auxiliary Cable 1200 Series VD Reference Manual...
  • Page 7 Theory of Operation How the Electronics Work Specifications Performance Specifications Safety Information General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet Index 1200 Series VD Reference Manual...
  • Page 8 1200 Series VD Reference Manual...
  • Page 9 Agilent 1200 Series Vacuum Degasser Reference Manual Installing the Vacuum Degasser Site Requirements Unpacking the Vacuum Degasser Optimizing the Stack Configuration Installing the Vacuum Degasser Flow Connections to the Vacuum Degasser Transporting the Vacuum Degasser Agilent Technologies...

  • Page 10: Installing The Vacuum Degasser

    C A U T I O N disconnect the instrument from line. Never use cables other than the ones supplied by Agilent Technologies to ensure WA R N I N G proper functionality and compliance with safety or EMC regulations.
  • Page 11 2.5 cm (1.0 inches) of space at either side and approximately 8 cm (3.1 inches) at the rear for the circulation of air and electric connections. If the bench should carry a complete Agilent 1200 Series system, make sure that the bench is designed to carry the weight of all the modules.
  • Page 12 Operating the instrumentation under conditions other than their intended use might result in a potential safety hazard or might damage the instrumentation. The Agilent 1200 Series vacuum degasser has been tested for evaporation of solvents into N O T E the atmosphere by an independent institute with approved methods.

  • Page 13: Unpacking The Vacuum Degasser

    The delivery checklist is shown below. To aid in parts identification, please see "Parts and Materials" on page 63. Please report missing or damaged parts to your local Agilent Technologies Sales and Service Office. Table 2 Vacuum Degasser Delivery Checklist...
  • Page 14 Accessory Kit Contents G1322-68705 Description Part Number Quantity Syringe 5062-8534 Syringe Adapter 9301-1337 Mounting Tool 0100-1710 Waste Tube 5062-2463 Connecting Tubes labeled A to D G1322-67300 * Reorder Number (pack of 10) ** Reorder Number (5m) 1200 Series VD Reference Manual...

  • Page 15: Optimizing The Stack Configuration

    (from point of solvent mixing to head of column) and dead volume (from point of injection to detector outlet). Solvent Cabinet Vacuum Degasser Quaternary Pump Instant Pilot Autosampler Column Compartment Detector Figure 1 Recommended Stack Configuration (Front View) 1200 Series VD Reference Manual...
  • Page 16 Remote cable Pressure output to recorder CAN Bus cable to handheld controller AC power CAN Bus cable for inter module communication LAN to Analog signal LC ChemStation to recorder Figure 2 Recommended Stack Configuration (Rear View) 1200 Series VD Reference Manual...

  • Page 17: Installing The Vacuum Degasser

    AUX output allows to monitor the vacuum pressure in the degasser chamber. An Agilent 1200 Series control module can be connected to the CAN bus at any of the modules in the system except for the degasser. The Agilent ChemStation can be connected to the system through one GPIB or LAN cable at any of the modules (except for the degasser), preferably at the detector.
  • Page 18 The power switch stays pressed in and a green indicator lamp in the power switch is ON N O T E when the vacuum degasser is turned ON. When the line power switch stands out and the green light is OF, the vacuum degasser is turned OFF. 1200 Series VD Reference Manual...

  • Page 19: Flow Connections To The Vacuum Degasser

    2 Remove the front cover by pressing the snap fasteners on both sides. Figure 5 Removing the Front Cover 3 If the vacuum degasser is not used with an Agilent 1200 Series pump, connect the waste tube from the accessory kit to the waste outlet and place into your waste system.
  • Page 20 Atmospheric gases can diffuse through the tubing and dissolve in the mobile phase N O T E solvents. For best chromatographic results, keep the length of tubing between the vacuum degasser and your pump as short as possible. 1200 Series VD Reference Manual...
  • Page 21 Bottle Head Assembly Solvent cabinet Vacuum Degasser Inlet tube Outlet tube Tube clip Purge valve Pump head Inlet adapter Waste tubing Outlet capillary to sampler MCGV Figure 7 Flow Connections to the Vacuum Degasser (with Quarternary Pump) 1200 Series VD Reference Manual...

  • Page 22: Transporting The Vacuum Degasser

    5 Pull syringe plunger to draw solvent out of vacuum degasser and tubing. Continue to draw solvent into syringe until the solvent channel is completely empty. 6 Repeat step 2 through step 5 for the remaining solvent channels. 1200 Series VD Reference Manual...
  • Page 23 Agilent 1200 Series Vacuum Degasser Reference Manual Optimizing Performance Introduction to the Vacuum Degasser When to Use a Vacuum Degasser? Operation Modes of the Vacuum Degasser Operational Hints for the Vacuum Degasser Solvent Information Prevent Blocking of Solvent Filters Agilent Technologies...

  • Page 24: Optimizing Performance

    Optimizing Performance Introduction to the Vacuum Degasser The Agilent 1200 Series vacuum degasser, model G1322A, comprises a 4-channel vacuum container, including 4 tubular plastic membranes, and a vacuum pump. When the vacuum degasser is switched on, the control circuit turns on the vacuum pump which generates a partial vacuum in the vacuum container.

  • Page 25: When To Use A Vacuum Degasser

    (e.g. RI detector or UV detector in the low UV range, both at maximum sensitivity). • Sample degradation. • Fluorescence Quenching due to dissolved oxygen. • Baseline drift in electrochemical detectors due to dissolved oxygen especially in reduction mode. 1200 Series VD Reference Manual...

  • Page 26: Operation Modes Of The Vacuum Degasser

    Depending on the vacuum sensor batch variations this will result in different voltage readings. Regardless of the readings of the various stages no adjustment should be performed on the degasser electronics. 1200 Series VD Reference Manual...

  • Page 27: Normal Operation Mode 1

    115 to 190 Torr. The pressure signal measured with the auxiliary cable is in the range between 600 to 800 mV. If the vacuum level of normal operation mode two cannot be achieved within 8 minutes the instrument turns into error mode. 1200 Series VD Reference Manual...

  • Page 28: Continuous Mode

    If the degasser cannot reach one of the normal operation modes within 8 minutes the status indicator turns red and the vacuum pump is turned OFF. 1200 Series VD Reference Manual...

  • Page 29: Operational Hints For The Vacuum Degasser

    3 Disconnect solvent outlet tube of the channel that is supposed to be primed from your pump. 4 Connect syringe adapter to solvent outlet tube. 5 Push syringe adapter onto syringe. 1200 Series VD Reference Manual...
  • Page 30 3 Set flow to required value of your application and close the purge valve. 4 Pump for approximately 10 minutes before starting your application. 5 Repeat step 1 through step 4 with other channels, where needed. 1200 Series VD Reference Manual...

  • Page 31: Solvent Information

    • Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, di-isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides. • Mixtures of carbon tetrachloride with 2-propanol or THF dissolve stainless steel. 1200 Series VD Reference Manual...

  • Page 32: Prevent Blocking Of Solvent Filters

    (35%) for one hour. • Thoroughly flush the filter with bidistilled water (remove all nitric acid). • Replace the filter. Never use the system without solvent filter installed. N O T E 1200 Series VD Reference Manual...

  • Page 33: Troubleshooting

    Agilent 1200 Series Vacuum Degasser Reference Manual Troubleshooting Status Indicators Hardware Symptoms Agilent Technologies...

  • Page 34: Status Indicators

    • An error condition is indicated, when the lamp is red. An error condition exists when the vacuum degasser detects an internal defect that does not allow the vacuum to be built up in a certain time frame (approximately 8 minutes). 1200 Series VD Reference Manual...
  • Page 35 To prevent any damage of the vacuum degasser, switch off the vacuum degasser and remove the solvent bottles from the solvent cabinet to stop any gravity-caused flow of solvent into the vacuum chamber. 1200 Series VD Reference Manual...

  • Page 36: Hardware Symptoms

    "Installing the Vacuum Degasser" page 17. The vacuum degasser itself will not be able to generate any error messages in the system logbook of the Agilent 1200 Series. The following pages describe hardware symptoms which help you to isolate the cause of a hardware failure.

  • Page 37: All Lamps Are Off And The Vacuum Degasser Appears Dead

    4 Remove the fuses from the fuse holders. 5 Ensure the fuse wires inside the fuses are not broken. If a test meter is available, check the resistance of each fuse. A good fuse shows a low resistance (approximately 0 Ohm). 1200 Series VD Reference Manual...
  • Page 38 7 Reinsert the fuse holders and the power cable. 8 Switch ON the power switch. If the previous steps did not solve the problem, change the control assembly (see "Exchanging the Degasser Control Assembly and the Sensor Assembly" page 60). 1200 Series VD Reference Manual...

  • Page 39: If The Status Indicator Is Red

    The following described failure conditions will appear during the start-up procedure before the red error LED is turned on. Every power cycling of the degasser will give you 8 to 16 minutes of time to diagnose the failure conditions described on the following pages: 1200 Series VD Reference Manual...

  • Page 40: If The Status Indicator Is Yellow And The Vacuum Pump Is Not Running

    CN2 to the pump). If the windings are broken or shortened replace the vacuum pump (see "Exchanging the Vacuum Pump" on page 57). 1200 Series VD Reference Manual...

  • Page 41: If The Status Lamp Is Yellow And The Vacuum Pump Runs Continuously

    3 Switch degasser into continuous mode by turning SW1 to 1. 4 To duplicate the normal operation of the degasser, turn ON the degasser, wait approximately 15 seconds, then connect the sensor assembly directly to the inlet of the vacuum pump. 1200 Series VD Reference Manual...
  • Page 42 (see "Exchanging the Solenoid Valve" on page 59). 6 Switch the degasser back to normal operation modes by turning SW1 back to 0. 7 Properly reconnect the tubings and reinstall the solenoid valve. 1200 Series VD Reference Manual...
  • Page 43 If the above described troubleshooting steps do not lead to an identification of the problem, replace the electronic control module with the pressure sensor assembly (see "Exchanging the Degasser Control Assembly and the Sensor Assembly" on page 60). 1200 Series VD Reference Manual...
  • Page 44 Troubleshooting 1200 Series VD Reference Manual...
  • Page 45 Agilent 1200 Series Vacuum Degasser Reference Manual Repairing the Vacuum Degasser Overview of the Repair Procedures Overview of Internal Parts Exchanging Internal Parts Agilent Technologies...

  • Page 46: Repairing The Vacuum Degasser

    WA R N I N G appropriate safety procedures (for example, goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. 1200 Series VD Reference Manual...
  • Page 47 Do not use an excessively damp cloth that liquid can drip into the vacuum degasser. Do not let liquid drip into the vacuum degasser. It could cause shock hazard and it WA R N I N G could damage the vacuum degasser. 1200 Series VD Reference Manual...

  • Page 48: Using The Esd Strap

    2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end. 3 Attach the copper foil to a convenient and exposed electrical ground. Figure 12 Using the ESD Strap 1200 Series VD Reference Manual...

  • Page 49: Removing And Refitting The Top Cover

    • Remove solvent cabinet from the vacuum degasser • Remove vacuum degasser from the stack. 2 Unclip the clips on the top cover . 3 Lift the cover away. Clip Clips 1200 Series VD Reference Manual...
  • Page 50 6 Replace the top cover. Ensure the clips are seated 7 Reinstall the front cover. correctly. Clips 8 Reinstall the vacuum degasser in your system stack and connect the cables and capillaries and turn on the vacuum degasser. 1200 Series VD Reference Manual...

  • Page 51: Assembling The Main Cover

    1 Place the top part on the bench and insert the left and right 2 Replace the cover. side into the top part. Front 3 Replace the vacuum degasser in the stack and reconnect the cables and capillaries. 4 Turn ON the vacuum degasser. 1200 Series VD Reference Manual...

  • Page 52: Exchanging The Status Light Pipe

    1 The status light pipe is clipped into the top cover. • Remove the front cover and top cover, see “Removing and Refitting the Top Cover” on page 49. 2 Replace the top cover, see “Removing and Refitting the Top Cover” on page 49. 1200 Series VD Reference Manual...

  • Page 53: Overview Of Internal Parts

    Control assembly Board clip Board clip Solenoid valve Sensor assembly Vacuum tube Vacuum pump Vacuum chamber Figure 13 Top View of Vacuum Degasser 1200 Series VD Reference Manual...

  • Page 54: Exchanging Internal Parts

    C A U T I O N to prevent damage always use an ESD protection (for example, the ESD wrist strap from the accessory kit) when handling electronic boards and components, see "Using the ESD Strap" on page 16 1200 Series VD Reference Manual...

  • Page 55: Exchanging The Vacuum Chamber

    Disconnect the solvent inlet tubes at the degasser inlet ports Remove the solvent cabinet from the vacuum degasser Drain any remaining solvents from the degassing channels before removing the WA R N I N G vacuum chamber from the vacuum degasser. 1200 Series VD Reference Manual...
  • Page 56 14 Slide the senor board back into the slots of the vacuum chamber and fix the clip. 15 Reconnect all inlet and outlet tubes to the vacuum chamber, see Figure 7 page 21. 16 Refit the cover, see “Removing and Refitting the Top Cover” on page 49. 1200 Series VD Reference Manual...

  • Page 57: Exchanging The Vacuum Pump

    Repairing the Vacuum Degasser Sensor assembly Vacuum chamber Figure 14 Exchanging the Vacuum Chamber Exchanging the Vacuum Pump When required If not generating sufficient vacuum or electrically defective Tools required Screwdriver Pozidriv #1 Part required Vacuum pump, G1322-60000 1200 Series VD Reference Manual...
  • Page 58 10 Connect the vacuum pump cable to the control assembly (CN2). 11 Refit the cover, see “Removing and Refitting the Top Cover” on page 49. Screws Fixing plate Vacuum pump Rubber foot and holder Figure 15 Exchanging the Vacuum Pump 1200 Series VD Reference Manual...

  • Page 59: Exchanging The Solenoid Valve

    9 Connect the solenoid cable to the control board (CN3). 10 Refit the cover, see “Removing and Refitting the Top Cover” on page 49. Screws Solenoid valve Holder plate Figure 16 Exchanging the Solenoid Valve 1200 Series VD Reference Manual...

  • Page 60: Exchanging The Degasser Control Assembly And The Sensor Assembly

    The degasser control assembly does not include any serviceable parts and should WA R N I N G not be opened. Sensor assembly and degasser control assembly are a matched pair and must be N O T E exchanged together. 1200 Series VD Reference Manual...
  • Page 61 13 Connect all the cables to the control board (sensor assembly cable to CN6, solenoid valve cable to CN3, pump cable to CN2). 14 Refit the cover, see “Removing and Refitting the Top Cover” on page 49. 1200 Series VD Reference Manual...
  • Page 62 Repairing the Vacuum Degasser Control module Coupler Sensor assembly Control Board Screws Screws Side Panel Screws Figure 17 Exchanging the Degasser Control Assembly 1200 Series VD Reference Manual...
  • Page 63 Agilent 1200 Series Vacuum Degasser Reference Manual Parts and Materials Vacuum Degasser Parts Cover Parts Sheet Metal Kit Power and Status Light Pipes Accessory Kit Cable Overview Agilent Technologies...

  • Page 64: Parts And Materials

    Parts and Materials Vacuum Degasser Parts Figure 18 Vacuum Degasser Parts 1200 Series VD Reference Manual...
  • Page 65 Sensor assembly (included in the control assembly) no PN Vacuum degasser control assembly G1322-66500 Fuse 500 mA 2110-0458 Board clip G1322-43100 Solenoid valve G1322-60003 Vacuum tubes G1322-60002 Fixing plate no PN Vacuum pump G1322-60000 Leak tray G1322-44100 Leak pan, degasser 5042-8567 1200 Series VD Reference Manual...

  • Page 66: Cover Parts

    Parts and Materials Cover Parts Table 6 Cover Parts Item Description Part Number Set of all plastic covers, top-side-base 5065-9989 Front cover 5065-9990 Logo plate, Agilent 1200 5042-8901 Tube clip 5041-8387 Figure 19 Cover Parts 1200 Series VD Reference Manual...

  • Page 67: Sheet Metal Kit

    Parts and Materials Sheet Metal Kit Table 7 Sheet Metal Kit Item Description Part Number Sheet metal kit, includes base and top plate G1322-68701 Figure 20 Sheet Metal Kit 1200 Series VD Reference Manual...

  • Page 68: Power And Status Light Pipes

    Power and Status Light Pipes Item Description Part Number Power switch coupler 5041-8383 Light pipe – power switch 5041-8382 Power switch button 5041-8381 Light pipe – status lamp 5041-8384 Figure 21 Power and Status Light Pipes 1200 Series VD Reference Manual...

  • Page 69: Accessory Kit

    Accessory Kit G1322-68705 Item Description Part Number Syringe 5062-8534 Syringe adapter 9301-1337 Mounting Tool 0100-1710 Solvent tubing kit (4 tubes degasser to pump) G1322-67300 Waste tube** 5062-2463 * Reorder number (pack of 10) **Reorder number (5m) 1200 Series VD Reference Manual...

  • Page 70: Cable Overview

    Parts and Materials Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure WA R N I N G proper functionality and compliance with safety or EMC regulations. Table 10 Cable Overview Type Description Part Number...

  • Page 71: Remote Cables

    Parts and Materials Remote Cables This cable provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent 1100 or 1200 Series modules. The other end is connected to the degasser. Connection to Agilent 1100 or 1200 Modules...

  • Page 72: Auxiliary Cable

    Parts and Materials Auxiliary Cable One end of this cable provides a modular plug to be connected to the vacuum degasser. The other end is for general purpose. Agilent 1200 Series Vacuum Degasser to General Purpose Table 12 Connector G1322-61600 Color...

  • Page 73: Theory Of Operation

    Agilent 1200 Series Vacuum Degasser Reference Manual Theory of Operation How the Electronics Work Agilent Technologies...

  • Page 74: How The Electronics Work

    The electronic control circuit uses + 12 V which is generated from the + 24 V. The pressure sensor is connected to the vacuum chamber and checks for the correct vacuum in the system. 1200 Series VD Reference Manual...
  • Page 75 8 minutes (error timer limit) the error driver is activated. The error driver will deactivate the vacuum pump and solenoid valve. The status lamp turns red and the error output on the remote connector will be activated. 1200 Series VD Reference Manual...
  • Page 76 The continuous mode overwrites all other operation modes of the degasser. When activated (switch SW1 on the electronic board or via the auxiliary cable) the vacuum pump is forced into continuous mode and is turned on as long as the degasser is switched ON. 1200 Series VD Reference Manual...
  • Page 77 & regulator Error output Yellow Status driver Not used Pressure Pressure driver signal +24V +12V Voltage regulator Power switch Fuse +24V Switching AC In power supply Fuse Figure 23 Block Diagram — Vacuum Degasser Control Assembly 1200 Series VD Reference Manual...
  • Page 78 Theory of Operation 1200 Series VD Reference Manual...

  • Page 79: Specifications

    Agilent 1200 Series Vacuum Degasser Reference Manual Specifications Performance Specifications Agilent Technologies...

  • Page 80: Performance Specifications

    Specifications Performance Specifications Table 13 Performance Specifications Agilent 1200 Series Vacuum Degasser Type Specification Maximum flow rate 10 ml/min per channel Number of channels Internal volume per channel Typically 12 ml per channel Materials in contact with solvent PTFE, PEEK pH range 1 –...
  • Page 81 Agilent 1200 Series VD Reference Manual Safety Information General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet This chapter provides additional information on safety, legal and web.

  • Page 82: A Safety Information

    Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuse holders must be avoided. 1200 Series VD Reference Manual...
  • Page 83 When working with solvents please observe appropriate safety procedures (e.g. goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. 1200 Series VD Reference Manual...
  • Page 84 A caution alerts you to situations that could cause a possible loss of data. Do not C A U T I O N proceed beyond a caution until you have fully understood and met the indicated conditions. 1200 Series VD Reference Manual...

  • Page 85: The Waste Electrical And Electronic Equipment (Weee) Directive (2002/96/Ec)

    I, this product is classed as a “Monitoring and Control instrumentation” product. Do not dispose off in domestic household waste To return unwanted products, contact your local Agilent office, or see www.agilent.com for more information. 1200 Series VD Reference Manual...

  • Page 86: Radio Interference

    If test and measurement equipment is operated with equipment unscreened cables and/or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises. 1200 Series VD Reference Manual...

  • Page 87: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) 1200 Series VD Reference Manual...

  • Page 88: Solvent Information

    For example, a 1-% solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). • Mixtures of carbon tetrachloride with 2-propanol or THF. 1200 Series VD Reference Manual...

  • Page 89: Agilent Technologies On Internet

    For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select “Life Sciences & Chemical Analysis Solutions” It will provide also the latest firmware of the Agilent 1200 Series modules for download. 1200 Series VD Reference Manual...
  • Page 90 Safety Information 1200 Series VD Reference Manual...

  • Page 91: Index

    9, 17, damaged, delay volume, interface cable, degasser, 1200 Series VD Reference Manual...
  • Page 92 19, solvent filters checking, cleaning, prevent blocking, solvent information, solvent outlet tubes, specification performance specifications, physical, stack configuration, 1200 Series VD Reference Manual...
  • Page 94 In This Book This manual contains technical reference information about the Agilent 1200 Series vacuum degasser. The manual describes the following: • installation, • optimizing performance, • troubleshooting, • repairing, • parts and materials, • theory of operation, and •...

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