Purging The Pump - Agilent Technologies 1200 Series User Manual

5 Connect the end of the solvent tube to the syringe adapter. Slowly draw a
6 Disconnect the solvent tube from the syringe adapter, and reconnect the
7 Repeat steps 4 to 6 for the three remaining solvent channels.
8 When all 4 solvent channels are manually primed, remove the paper towel

Purging the Pump

1 Make sure that the 1/8 inch plastic waste tube is tightly connected to the
2 Turn on the LC System. All system parameters should be set to default. The
3 Initialize the system. Then, access the pump controls and make sure the
4 Access the pump Purge control. Set up a purge table which will purge all
When the pump has been turned off for a certain time (for example, overnight), oxygen will
N O T E
re-diffuse into the channels between the degasser and the pump. It is suggested to purge
each channel at 2500 µl/min for 1 minute at the beginning of each day.
1200 Series Nano Pump User Manual
syringe volume (20 ml) from the solvent tube.
tube to the A1 port of the solvent selection valve. Eject the syringe contents
into an appropriate waste container.
from the pump leak tray. Make sure that the pump leak sensor is dry before
turning on the pump.
barbed waste fitting of the pump EMPV, and routed to an appropriate waste
container.
degasser should also be turned on at this time.
pump mode is set to Normal.
channels for 5 minutes each, at a flow of 2500 µl/min. Then, start the purge.
Getting started 2
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