Agilent Technologies 1200 Series Reference Manual page 29

1 Open the purge valve of your pump (by turning it counterclockwise) and set
2 Flush the vacuum degasser and all tubes with at least 30 ml of solvent.
3 Set flow to required value of your application and close the purge valve.
4 Pump for approximately 10 minutes before starting your application.
5 Repeat
Table 5
Activity
After an installation
When switching between
reverse phase and normal
phase (both times)
After an installation
To clean the system when
using buffers
After a solvent change
After the installation of normal
phase seals (P/N 0905-1420)
1200 Series QP Reference Manual
flow rate to 3-5 ml/min.
step 1 to step 4
Choice of Priming Solvents for Different Purposes
for other solvent channels, where needed.
Solvent
Isopropanol
Isopropanol
Ethanol or Methanol
Bidistilled water
Bidistilled water
Hexane + 5% Isopropanol
Installing the Pump
Comments
Best solvent to flush air out of
the system
Best solvent to flush air out of
the system
Alternative to Isopropanol
(second choice) if no
Isopropanol is available
Best solvent to re-dissolve
buffer cristals
Best solvent to re-dissolve
buffer cristals
Good wetting properties
1
29