Purging The Pump - Agilent Technologies InfinityLab LC Series User Manual

3 Using the Pump

Purging the Pump

Purging the Pump

When the solvents have been exchanged or the pumping system has been turned
off for a certain time (for example, overnight) oxygen will re-diffuse into the
solvent channel between the solvent reservoir, vacuum degassing unit (when
available in the system) and the pump. Solvents containing volatile ingredients
will slightly lose these. Therefore purging of the pumping system is required
before starting an application.
1 Initiate a purge in the controlling software with a Purge flow set to 3 mL/min
2 Flush all tubes with at least 15 mL of solvent.
Table 5 Choice of Priming Solvents for Different Purposes
Activity
After an installation
When switching between reverse phase and
normal phase (both times)
After an installation
To clean the system when using buffers
After a solvent change
Before turning off system for an extended
period of time
The pump should never be used for priming/purging empty tubings (never let the
NOTE
pump run dry). Use a syringe to draw enough solvent for completely filling the
tubings to the pump inlet before continuing to purge with the pump.
1290 Infinity II High-Speed Pumps User Manual
per channel.
Solvent
Isopropanol
Isopropanol
Ethanol or Methanol
Bidistilled water
Bidistilled water
50 % methanol or 50 %
isopropanol in water,
without additives
Comments
Best solvent to flush air out of the system
Isopropanol is miscible with both normal phase
and reverse phase solvents.
Alternative to Isopropanol (second choice) if no Iso-
propanol is available
Best solvent to re-dissolve buffer crystals
Best solvent to re-dissolve buffer crystals
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