Infors HT Labfors 5 Operating Manual page 8

Labfors 5 - Version for Microorganisms
Table of Contents
Page 6 of 205
8.2.15 Checking the Hoses and Hose Connections
....................................................................148
9 Fermentation/Cultivation ...................................................149
9.1 Preparing the Medium ................................................149
9.2 Sampling ....................................................................150
9.3 Inoculation ..................................................................153
9.3.1 Inoculation with Inoculation Needle ............154
9.3.2 Inoculation with a Syringe ...........................155
9.3.3 Inoculation Using Dip Tube / Addition Port
Adapter .......................................................155
9.4 Harvest .......................................................................156
9.5 Emptying the Culture Vessel ......................................157
9.6 Emptying the Reagent Hoses ....................................157
9.7 Switching off the Equipment ......................................157
9.8 Autoclaving the Culture Vessel After
Fermentation/Cultivation ............................................158
10 Cleaning and Maintenance ................................................160
10.1 Cleaning Agent and Disinfectant ................................160
10.2 Cleaning the Culture Vessel - Routine Cleaning .......160
10.3 Removing the Vessel Top Plate and Accessories .....162
10.3.1 Removing the Exit Gas Cooler ...................162
10.3.2 Removing the Sensors ...............................162
10.3.3 Removing Hoses, Filters and Pump Heads
....................................................................163
10.3.4 Removing Blanking Plugs ...........................164
10.3.5 Removing Threaded Adapters ....................164
10.3.6 Removing the Inoculation Needle, Septum
Collar and Septum ......................................164
10.3.7 Removing Addition Port Adapters ..............165
10.3.8 Removing the Vessel Top Plate .................165
10.3.9 Removing the Pocket for Temperature Sensor
(Pt100) ........................................................167
10.3.10 Removing the Sparger and the Dip Tube(s)
....................................................................167
10.3.11 Removing the Baffles .................................167
10.3.12 Removing the Impellers ..............................168
10.3.13 Removing the Stirrer Shaft .........................168
10.4 Cleaning and Storing Individual Parts ........................169
10.5 Cleaning the Sensors .................................................170
10.6 Cleaning the Hoses and Pump Heads .......................171
10.7 Cleaning the Super Safe Sampler .............................171
10.8 Cleaning the Exit Gas Cooler .....................................172
10.9 Cleaning the Basic Unit and Operating Panel ...........172
22 January 2019