Checklist Before Autoclaving - Infors HT Labfors 5 Operating Manual

Labfors 5 – Version for Microorganisms
Before Fermentation/Cultivation

8.1.25 Checklist Before Autoclaving

Culture vessel
All necessary O-rings are fitted.
All unused ports are closed with blanking plugs
Port for inoculation is equipped with septum, septum collar and blanking plug
There is liquid in the culture vessel (autoclavable medium or approx. 10 mL water per litre working volume).
Mechanical seal is lubricated.
Drive hub is lightly capped with aluminium foil.
Double walled culture vessels: The water overflow hose of the vessel jacket is open, it is NOT kinked or
clamped off.
Reagent bottles, hoses and pumps
Reagent bottles are exclusively filled with autoclavable reagents, correctly labelled and connected with the
culture vessel and the pump heads via hoses.
Reagent bottles are equipped with sterile filters for pressure equalisation
Reagent bottles are placed in reagent bottle and pump holder and pump heads are placed on the holder
with a mounting plate.
Inoculation needles
Inoculation needle is connected to appropriate container for inoculum with a silicone hose.
Inoculation needle is packed in sterile cover or wrapped in aluminium foil.
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3. Fit the mounting plate with the pump heads onto the reagent
bottle and pump holder.
Check and ensure the following items before autoclaving:
22 January 2019