Calibrating The Ph Sensor - Infors HT Labfors 5 Operating Manual

Labfors 5 – Version for Microorganisms
Before Fermentation/Cultivation

8.1.17.1 Calibrating the pH Sensor

Page 120 of 205
Mount the sensors in such a way that they cannot come in

contact with other components or the glass vessel.
If the mounting depth of is adjustable (mounting with electrode

holder/clamping adaptor), make sure the mounting depth is
set correctly prior to autoclaving, as later adjustment repre-
sents a contamination risk.
pH sensor
Calibrate the pH sensor on the operating panel before mounting
and autoclaving.
pO sensor
2
Mount the pO sensor in such a way that it has good access to the
2
flow and there is no risk of bubbles collecting. (Calibration is car-
ried out AFTER autoclaving!)
pH sensor & pO sensor METTLER
2
Cover the sensor heads of METTLER pH sensor and pO2 sensors
with aluminium foil during autoclaving.
sensor HAMILTON
pH sensor & pO
2
ATTENTION
Risk of damage to the (HAMILTON) pH and pO
ing the sensor heads with aluminium foil during autoclaving may
lead to water gathering under the film, thus damage the contacts
on the sensor head.
pH and pO sensor heads should NOT be covered with alumin-
2
ium foil during autoclaving.
For details on the safety, technical data, usage and maintenance
requirements for the pH and pO
mentation provided by the manufacturers.
For a reliable pH measurement, a 2-point calibration with an upper
and lower reference buffer must be carried out before each cultiva-
tion. The pH sensor must be calibrated before autoclaving. This is
carried out on the operating panel and described in detail in the
separate operating manual of the touch screen software.
sensor. Cover-
2
sensors, see the separate docu-
2
22 January 2019