Sparger - Infors HT Labfors 5 Operating Manual

Labfors 5 – Version for Microorganisms
Accessories

5.4 Sparger

Page 66 of 205
sample has to be processed under sterile conditions. For sampling,
the same non-sterile syringe can be used repeatedly, without fear
of contamination of the culture vessel.
Aseptic Sampling
For each sample, use a new, sterile syringe with Luer Lock fitting,
in order to ensure the sterility of the sample.
Sterile syringes are consumables and therefore not included in the
set.
INFORMATION
The use of another syringe is also possible. But a syringe with
Luer lock prevents unwanted movement of the syringe.
Before fitting the syringe, disinfect the sample valve. Fort this,

spray a commercially available disinfectant onto the valve.
After spraying and after each sampling, close the the sample

valve with a sterile Luer-Lock cap (Dead End Cap) to keep the
valve and sample sterile.
The caps are not included in the kit. Very convenient to use are so-
called combi-caps that fit on male and female connectors alike.
Caps that are vented and made of steam sterilisable material can
also be fitted during autoclaving.
The gas is fed directly into the medium via a sparger by default.
The sparger is mounted in a 12 mm / Pg13.5 port in the vessel top
plate using a clamping adapter, and connected to the gassing sys-
tem on the basic unit via a silicone hose with a sterile filter.
22 January 2019