Preparing The Dip Tube/Addition Port Adapter For Inoculation; Equipping Port With Septum Collar And Septum For Inoculation - Infors HT Labfors 5 Operating Manual

Labfors 5 – Version for Microorganisms
Before Fermentation/Cultivation
Procedure

8.1.12 Preparing the Dip Tube/Addition Port Adapter for Inoculation

Procedure

8.1.13 Equipping Port with Septum Collar and Septum for Inoculation

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1. Remove protective caps from the inoculation needle.
2.
Keep the septum collar ready for use.
3. Connect the inoculation needle via silicone hose with an ap-
propriate container for the inoculum.
4. Put the inoculation needle in a sterile cover or wrap it up in
some aluminium foil.
If later inoculation is to be carried out by means of a dip tube or
addition port adapter, proceed as follows:
1. Fit the dip tube with the clamping adapter or addition port
adapter in the port.
2. Place a piece of silicon hose onto the dip tube/addition port
adapter.
3. Equip the hose for a sterile hose connection. (Depending on
the application: rapid coupling, sterile connector or weldable
hose with sterile filter).
4. Secure the hose transition points with cable ties.
A port in the vessel top plate must be appropriately prepared for
later inoculation. For this, it must be equipped with septum collar
and septum.
22 January 2019