Infors HT Labfors 5 Operating Manual page 154

Bench-top bioreactor - version for microorganisms
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Labfors 5 – Version for Microorganisms
Fermentation/Cultivation
Procedure:
Page 152 of 205
a)
Unscrew the syringe from the valve.
b)
Hold the syringe with the plunger downwards so that the me-
dium remains in the syringe.
c)
Push the air out of the syringe.
d.
Screw the syringe onto the sample valve.
e)
Draw in again.
7.
Attach the clamp to the sampling hose.
Rinsing the dip tube with sterile air
The dip tube and its sampling hose can be filled with sterile air af-
ter taking a sample.
INFORMATION
Only use a clean and dry syringe to avoid blocking the sterile fil-
ter. This syringe can be reused as often as desired, since air is
provided via a sterile filter.
To do so, proceed as follows:
1.
Insert the syringe onto the hose at the sterile filter and push
air through.
The remaining liquid in the hose and in the dip tube is pushed
back into the vessel.
2.
Remove the syringe from the sterile filter to fill it with air again.
3.
Repeat steps 1 and 2 as many times as necessary until bub-
bles rise out of the dip tube.
22 January 2019

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