Gassing; End Of Test - Infors HT Labfors 5 Operating Manual

Labfors 5 – Version for Microorganisms
Installation and Initial Operation
7.7.5

Gassing

Procedure
7.7.6

End of Test

Procedure
Page 104 of 205
The temperature of the liquid (water) in the vessel jacket starts
increasing.
2.
Wait until the temperature has adjusted to the setpoint.
For the rest of the procedure, allow the bioreactor to run with the
temperature switched on and the stirrer running.
Bioreactor is running with temperature and stirrer switched on
To test the gassing, proceed as follows:
1. If applicable, slowly open the rotameter needle valve.
2. If applicable, set a low setpoint in the appropriate parameter
Flow (depending on the configuration) and switch the parame-
ter on.
3.
If applicable, ensure that all other gas parameters (e.g.
Gasmix, GM Flow etc.) are switched off.
If the gassing is working, air bubbles now form in the water in
the culture vessel.
After all parameter setpoints have been reached, the test can end
here.
Proceed as follows:
1. Stop the bioreactor on the operating panel and shut down the
system.
2. Switch the equipment off at the main switch.
ATTENTION
Switching the equipment off at the main switch without previ-
ously stopping the bioreactor and shutting down the system on
the operating panel may lead to damage of the operating panel!
3. Shut off the supply lines.
4. Let the motor cool down.
22 January 2019