Blood Component Separation - Beckman Coulter Avanti J Series Instructions For Use Manual

Blood Component Separation

Separation of Blood Components by Centrifugation
Figure D.1 Blood Component Separation
soft spin
Whole Blood
hard spin
Beckman Coulter has centrifuges, rotors, and accessories designed to fit the special needs of blood
component processing. Several rotors are available to accommodate single, double, triple, and quad
blood bags. Blood bag cups rest in the rotor bucket and simplify processing, since they eliminate the
need to remove buckets after each run. They also minimize clean-up downtime if a bag breaks—
simply remove the cup and resume the run. Refer to the applicable rotor manual for blood bag cups
used with each rotor.
in a variety of J6 series centrifuges.
D-4
Donor blood is collected in plastic bags with one or more satellite bags
(double, triple, or quad packs) containing anticoagulant and preservative.
After each centrifugation run, the sedimented fraction is squeezed into its
respective satellite bag. Common anticoagulants and preservatives
include citrate-phosphate-dextrose (CPD), citrate-phosphate-dextrose-
citrate-citric acid-adenine (CPDA-1), saline-adenine-glucose-minitol
(SAG-M), and dextrose-sodium chloride-mannitol-adenine (ADSOL).
Blood separations occur during centrifugation because of particle
sedimentation. Using sedimentation theory, users can calculate
sedimentation rates. For example, red blood cells settle at the
approximate rate of 2 cm per hour in aqueous medium at 1 g, with higher
force fields increasing the settling rate. Note that blood cells should not be
subjected to high centrifugal force fields, as the cells can be damaged.
hard spin
Platelet-rich
Plasma
Red Blood Cells
slow-thaw,hard
Plasma
(Cell-Free)
Packed Cells
Table D.2 lists blood bank methods that can be used for separating components
Plasma
(Platelet-depleted)
Platelet
Concentrate
Plasma
(Cryoprecipitate
Removed)
Cryoprecipitated
Antihemophilic
Factor (Factor VIII)
PN JR-IM-10AG