Using Fixed-Angle Rotors; Introduction; Description - Beckman Coulter Avanti J Series Instructions For Use Manual

Introduction

This section contains instructions for using fixed-angle rotors in J series centrifuges. In addition to these
instructions, observe procedures and precautions provided in the applicable rotor and centrifuge manuals.
Refer to CHAPTER 2
sealing or capping requirements and for sample recovery procedures. Refer to
on the care of rotors and accessories.

Description

Fixed-angle rotors (see
useful for pelleting subcellular particles and in short-column banding of viruses and
subcellular organelles. Refer to
Tubes in fixed-angle rotors are held at an angle (usually 20 to 45 degrees) to the axis of
rotation. The tube angle shortens the particle pathlength compared to swinging-
bucket rotors, resulting in reduced run times. Tubes can be placed directly in a rotor
cavity if the diameters of the tube and the cavity are the same. Using adapters, more
than one type and size of tube can be centrifuged together, provided that the load is
properly balanced.
O-rings, made of Buna N rubber, are located in the rotor lid. The O-rings help to
maintain atmospheric pressure inside a fixed-angle rotor during centrifugation, when
they are properly lubricated.
A tie-down device or lid-locking knob is used to secure the rotor to the centrifuge
drive spindle hub before the run begins.
PN JR-IM-10AG

Using Fixed-Angle Rotors

for labware selection information, and
Figure 4.1) are general-purpose rotors that are especially
Table 4.1
CHAPTER 3 for recommended filling and
CHAPTER 7
for general rotor specifications.
CHAPTER 4
for information
4-1