Beckman Coulter JS-5.2 Instructions For Use Manual
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Beckman Coulter JS-5.2 Instructions For Use Manual

Swinging-bucket rotors for use in j6 series/avanti jxn-26, j-26xp series centrifuges
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J6-TB-006AP
September 2016
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
Instructions For Use
JS-5.2 and JS-4.0
Swinging-Bucket Rotors
JS-5.2 Rotor for Use in
Beckman Coulter J6 Series
Centrifuges
JS-4.0 Rotor for Use in
Beckman Coulter Avanti JXN-26,
J-26XP Series and J6 Series Centrifuges

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Summary of Contents for Beckman Coulter JS-5.2

  • Page 1 Instructions For Use JS-5.2 and JS-4.0 Swinging-Bucket Rotors JS-5.2 Rotor for Use in Beckman Coulter J6 Series Centrifuges JS-4.0 Rotor for Use in Beckman Coulter Avanti JXN-26, J-26XP Series and J6 Series Centrifuges J6-TB-006AP September 2016 Beckman Coulter, Inc. 250 S. Kraemer Blvd.
  • Page 2 J6-TB-006AP (September 2016) Copyright © 2016 Beckman Coulter, Inc. Avanti, Beckman Coulter, and the stylized logo are trademarks of Beckman Coulter, Inc. and are registered in the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.

  • Page 3: Safety Notice

    These rotors were developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter ultracentrifuge/ rotor system. Their safety or reliability cannot be assured if used in an ultracentrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter ultracentrifuge that has been modified...
  • Page 4 Beckman Coulter. Use of other manufacturers’ components or accessories in the rotors may void the rotor warranty and should be prohibited by your laboratory safety officer. If tubes, microplates, or other labware made by manufacturers other than Beckman Coulter are used, reduce rotor speed to prevent breakage.

  • Page 5: Table Of Contents

    Contents Safety Notice, iii Alerts for Danger, Warning, Caution, and Note, iii Safety Information for the JS-5.2 and JS-4.0 Rotors, iii JS-5.2 and JS-4.0 Swinging-Bucket Rotors, 1 Specifications for the JS-5.2 Rotor, 1 Specifications for the JS-4.0 Rotor, 2 Description, 2...
  • Page 6 Components, 25 Buckets, 26 Multi-Disc Adapters, 26 Decontamination, 27 Sterilization and Disinfection, 27 Returning a Rotor, 28 Supply List, 28 Replacement Rotor Parts, 29 Other, 29 Beckman Coulter, Inc. J-Series Swinging-Bucket Rotor Warranty Replacement, 1 Conditions, 1 Disclaimer, 2 Related Documents...
  • Page 7 Carriers, 17 Examples of the Effects on the Horizontal Swing of Buckets Caused by Proper and Improper Weight Distribution, 18 Assembling a Multi-Disc Adapter, 18 Brackets (Vertical Supports) for the JS-5.2-Style Multi-Disc Adapters and the GH-3.8-Style Modular Disk Adapters, 19...
  • Page 8 Tables Tables Available Tubes and Bottles for the JS-5.2 and JS-4.0 Swinging- Bucket Rotors, 5 Multi-Disc Adapters Used with Tubes and Bottles, 8 Blood Bag Cups, 9 Labware for Use with Microplate Carriers, 10 Relative Centrifugal Fields, 22 Temperature Compensation Settings for J6 Series...

  • Page 9: Js-5.2 And Js-4.0

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Specifications for the JS-5.2 Rotor NOTE The JS-5.2 rotor is used in Beckman Coulter J6 series centrifuges only. It cannot be used in Avanti J series centrifuges. Maximum speed 5200 RPM Critical speed range 600 to 800 RPM Maximum solution density 1.2 g/mL...

  • Page 10: Specifications For The

    ). After substitution: RCF = 1.12r (RPM/1000) Description These Beckman Coulter rotors have been manufactured in an ISO 9001 or 13485 facility for use with the specified Beckman Coulter ultracentrifuges. The JS-5.2 and JS-4.0 swinging-bucket rotors are rated for maximum speeds of 5200 and 4000 RPM, respectively.

  • Page 11: Preparation And Use

    See the Warranty at the back of this manual for warranty information. Preparation and Use Specific information about the JS-5.2 and JS-4.0 rotors is given here. Use the J Series Rotors and Tubes Manual (publication JR-IM) along with this rotor manual for complete rotor and accessory information.

  • Page 12: Buckets And Accessories

    • Opposing containers must be filled to the same level with liquid of the same density. Buckets and Accessories The round buckets used in the JS-5.2 and JS-4.0 rotors can hold bottle adapters, Multi-Disc adapters to accommodate tubes of various sizes, and blood bag cups.

  • Page 13: Blood Bag Cups

    Refer to Appendix A in Rotors and Tubes for chemical compatibilities of tube, bottle, and accessory materials. Table 1 Available Tubes and Bottles for the JS-5.2 and JS-4.0 Swinging-Bucket Rotors Tube...
  • Page 14 JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use Table 1 Available Tubes and Bottles for the JS-5.2 and JS-4.0 Swinging-Bucket Rotors (Continued) Tube Max Fill Required Accessory Volume Speed Dimensions/ Description Part Description Part (mL) and Volume/ Number Number 96  130 mm...
  • Page 15 JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use Table 1 Available Tubes and Bottles for the JS-5.2 and JS-4.0 Swinging-Bucket Rotors (Continued) Tube Max Fill Required Accessory Volume Speed Dimensions/ Description Part Description Part (mL) and Volume/ Number Number 29  104 mm...

  • Page 16: Multi-Disc Adapters Used With Tubes And 3 Blood Bag Cups

    Above 20°C fill polypropylene tubes at least half full. c. Maximum speeds listed are for the JS-5.2 rotor, and are guidelines only. These speeds have been achieved in reliability tests at Beckman Coulter, but, because of manufacturing variances, no guarantee of performance or fit is expressed or implied. The maximum speed of all tubes and bottles in the JS-4.0 rotor is 4000 RPM.

  • Page 17: Temperature Limits

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use Table 2 Multi-Disc Adapters Used with Tubes and Bottles Typical Tube/ Number of Number of Adapter Color Max Tube Dimensions Bottle Sizes Tubes per Tubes per Part Used Adapter Rotor Number Diameter...

  • Page 18: Microfuge Tubes

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use other than sucrose and glycerol. Stainless steel tubes are very strong and can be centrifuged when filled to any level. Stainless steel tubes can be used indefinitely if they are undamaged and not allowed to corrode.

  • Page 19: Operation

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use Table 4 Labware for Use with Microplate Carriers Description Volume Part Required Accessory Rack Number Description Part Number Microfuge tube, 1.5 mL 356093 Rack insert, 373696 373661 polypropylene, yellow white (holds 24 tubes) Microfuge tube, 1.5 mL...

  • Page 20: Rotor Drive Pin Location And 2 Centrifuge Drive Spindle Hub

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use Figure 1 Rotor Drive Pin Location and Orientation 1. Rotor Drive Hole 3. Drive Pins 2. Centrifuge Drive Hub 4. Drive Hub Teeth Figure 2 Centrifuge Drive Spindle Hub Configuration Older Model Centrifuges —...

  • Page 21: Using The Tie-Down Bolt And Tie-Down Tool To Tighten The Rotor Onto The Drive

    (878439), as shown in Figure JS-5.2 rotor only: Place the lid onto the rotor, carefully seating it on the windshield. Make sure that the tie-down bolt pin protrudes into the hole in the lid knob. b. If you keep the rotor in the centrifuge, re-tighten the tie-down bolt before each run.

  • Page 22: Installing The Buckets Or Microplate Carriers

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use Figure 4 Using the Tie-Down Screw and Torque Bar to Tighten the Rotor onto the Drive Hub (Older Rotors) 1. Torque Bar (878439) 2. Rotor Tie-Down Screw (339031) Installing the Buckets or Microplate Carriers Lubricate the contact area between the buckets or carriers and the pivot pins on the rotor yoke as described under Inspection.

  • Page 23: Loading The Buckets And Carriers

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use CAUTION All four positions on the rotor yoke must contain either a bucket or a microplate carrier (loaded or unloaded) during a run. Never run the rotor without all four positions being filled.
  • Page 24 JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use During a run, buckets (or carriers) swing 90 degrees from their at-rest position. The pivotal axis of a bucket can be imagined as a line extending across the bucket from one pivot pin to the other. If a...

  • Page 25: Examples Of Correctly And Incorrectly Loaded Buckets And Carriers

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use Figure 5 Examples of Correctly and Incorrectly Loaded Buckets and Carriers Unbalanced Load Balanced Load Pivotal Axis of Bucket Center of Rotation Incorrect Correct Examples of Nonsymmetrically Examples of Symmetrically Loaded Trays...

  • Page 26: Loading Buckets

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use Figure 6 Examples of the Effects on the Horizontal Swing of Buckets Caused by Proper and Improper Weight Distribution AT REST AT SPEED AT REST AT SPEED Proper Weight Distribution Improper Weight Distribution Loading Buckets Load buckets before or after they are installed on the rotor yoke.

  • Page 27: Brackets (Vertical Supports) For The Js-5.2-Style Multi-Disc Adapters And The Gh-3.8-Style Modular Disk

    Do not intermix Beckman Coulter GH-3.8 rotor style Multi-Disc adapters with adapters for the JS-5.2 and JS-4.0 rotors. The adapters are similar in appearance (see Figure 8), but they have very different weights. Mixing them during a run can cause imbalance.

  • Page 28: Loading Adapters

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use Loading Adapters First, test to make sure that the tubes or bottles in the adapters will not contact the rotor yoke during centrifugation. a. Place empty tubes or bottles in an adapter and then place the adapter in a bucket on the rotor yoke.

  • Page 29: Loading Microplate Carriers

    1.2 g/mL or less. Rotor speeds must be reduced from the rated maximum under any of the following circumstances: • If the JS-5.2 rotor is run without a lid, limit the speed to 1450 RPM. The additional windage created by not using the lid will overload the centrifuge drive and refrigeration systems above this speed.

  • Page 30: Run Temperature

    Enter the run temperature according to the instructions in your centrifuge instruction manual. No additional input is required. Microprocessor-Controlled J6 Series Centrifuges Enter rotor code (for the JS-5.2) or rotor code (for the JS-4.0) and then follow the steps below. Press the TEMP key on the centrifuge control panel and then use the keypad to enter the sample temperature.

  • Page 31: Temperature Compensation Settings For J6 Series

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Preparation and Use Table 6 Temperature Compensation Settings for J6 Series Centrifuge Rotor Speed Required Sample Temperature (°C, green bar) (RPM) 2°C 4°C 8°C 10°C 15°C 20°C 30°C 5200 –3 –1 4000 –1 3000 2000 and below...

  • Page 32: Care And Maintenance

    Inspect the O-ring and plug for nicks, abrasions, and other damage. Replace damaged components with Beckman Coulter parts only; do not use a substitute for the O-ring—it has been specifically selected for this application.

  • Page 33: Cleaning

    Also, lightly grease the inside top 1.2 cm (0.5 in.) of the bucket. Refer to Appendix A in Rotors and Tubes for the chemical resistances of rotor and accessory materials. • Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center. Cleaning Under normal conditions, remove the rotor from the centrifuge at least once a week for cleaning and lubrication.

  • Page 34: Buckets

    JS-5.2 and JS-4.0 Swinging-Bucket Rotors Care and Maintenance Approximately once a week (or every 80 runs), clean the pins and bucket pin sockets, then lubricate as described under Maintenance. Most laboratory detergents are too harsh for use on aluminum rotors because they can damage the anodized surface.

  • Page 35: Decontamination

    See Appendix A in Rotors and Tubes for more information regarding chemical resistance of tubes, bottles, and accessories. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer.

  • Page 36: Returning A Rotor

    Before returning a rotor or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. This form may be obtained from your local Beckman Coulter sales office. The form, entitled Returned Material Authorization (RMA) for United States returns or Returned Goods Authorization (RGA) for international returns, should contain the following information: •...

  • Page 37: Replacement Rotor Parts

    Aeroseal cover for round buckets (1) 343686 Rotor cover assy (JS-5.2 only) 339085 Other NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com. Description Part Number Blood bag cups Table 3 Microplate carriers (set of 2, includes rubber pads)
  • Page 38 JS-5.2 and JS-4.0 Swinging-Bucket Rotors Supply List 1-30 J6-TB-006AP...

  • Page 39: Beckman Coulter, Inc

    Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc., terms and conditions of sale in effect at the time of sale, Beckman Coulter, Inc. agrees to correct either by repair, or, at its election, by replacement, any defects of material or workmanship which develop within seven (7) years after delivery of a J series rotor to the original buyer by Beckman Coulter, Inc.

  • Page 40: Disclaimer

    Beckman Coulter, Inc. J-Series Swinging-Bucket Rotor Warranty Disclaimer purchase plus seven (7) years. Use of a bucket after such expiration date voids Beckman Coulter’s warranty obligations with respect to any rotor and/or centrifuge in which such a bucket is used.

  • Page 42: Related Documents

    Related Documents Rotors and Tubes for Beckman Coulter J2, J6, Additional References Avanti J Series Centrifugtes (JR-IM-10) • Chemical Resistances for Beckman Coulter • Rotors Centrifugation Products (IN-175) • • Tubes, Bottles, and Accessories Beckman Coulter High-Performance and High-Capacity Centrifuges catalog (BR-8102) •...

This manual is also suitable for:

Js-4.0

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