Using Swinging-Bucket Rotors; Introduction; Description - Beckman Coulter Avanti J Series Instructions For Use Manual

Introduction

This section contains instructions for using swinging-bucket rotors in J series centrifuges. In addition to
these instructions, observe procedures and precautions provided in the applicable rotor and centrifuge
manuals.
Refer to CHAPTER 2
sealing requirements and for sample recovery procedures. Refer to
of rotors and accessories.

Description

Swinging-bucket rotors (see
separations, either isopycnic or rate zonal. Refer to
specifications. A tie-down device or lid-locking knob is used to lock the rotor to the
centrifuge drive hub before the run begins.
Tubes or bottles in swinging-bucket rotors are held in the rotor buckets that are
attached to the rotor body by hinge pins. The buckets swing out to horizontal
position as the rotor is accelerated, and stay horizontal until rotor deceleration
begins. During deceleration, the buckets gradually return to vertical position.
NOTE Although
PN JR-IM-10AG

Using Swinging-Bucket Rotors

for tube selection information, and
Figure
rotor components and accessories made by other manufacturers may
fit in the Beckman Coulter rotor you are using, their safety in the rotor cannot be
ascertained by Beckman Coulter. Use of other manufacturers' components or
accessories in the rotor may void the rotor warranty, and should be prohibited by your
laboratory safety officer. Only the components and accessories listed in the applicable
rotor manual should be used.
CHAPTER 3 for recommended labware filling and
CHAPTER 7 for information on the care
5.1) are normally used for density gradient
Table 5.1 for general rotor
CHAPTER 5
5-1