Shelf Life; Freezing; Separation Of Blood Components By Centrifugation; Blood Component Storage - Beckman Coulter Avanti J Series Instructions For Use Manual

Shelf Life

Table D.1 lists approximate storage times for the separated components.
Table D.1 Blood Component Storage
Component
RBC with ADSOL
RBC with SAG-M
RBC with CPDA-1
RBC without preservative
RBC frozen without addition of
cryoprotective agent
Plasma—frozen
a. ADSOL = dextrose-sodium chloride-mannitol-adenine.
b. SAG-M = saline-adenine-glucose-minitol.
c. CPDA-1 = citrate-phosphate-dextrose-citrate-citric acid-adenine.

Freezing

A cryoprotective agent such as glycerol can be added to extend the life of frozen red blood cells.
These cells can then be stored for up to 3 years at –80°C. Prior to use the cells are thawed and the
glycerol is removed by washing.
If the plasma will not be separated within 15 hours of collection, it must be frozen within 6 hours of
collection. Freezing must be carried out in a flash freezer with complete freezing accomplished
within 1 or 2 hours of the time it is placed in the freezer.

Separation of Blood Components by Centrifugation

Centrifugation is the primary method for processing blood because it offers the required high
throughput, reproducibility, and versatility. Most blood components can be separated in one or
two runs. Generally, two types of centrifugation runs are performed (see
short centrifugation runs (3 to 5 minutes) at low g-forces (2000 to 3000 × g) at ambient temperature,
are used to keep small cells or platelets in suspension while the larger cells sediment. This type of
run is used to obtain platelet-rich plasma and red blood cell concentrate from whole blood.
Hard spin runs are longer (5 to 7 minutes), at higher g-forces (4000 to 5000 × g), at ambient
temperature or at 4°C, and are used to separate fresh plasma from cellular components. Soft spin
and hard spin techniques are often combined.
PN JR-IM-10AG
Approximate
Storage Life
a 42 days
35 days
b
c 25 days
21 days
10 days
1 year
Blood Component Separation
Shelf Life
Figure D.1). Soft spin runs,
D-3
D