General Filling And Sealing Or Capping Requirements - Beckman Coulter Avanti J Series Instructions For Use Manual

Tubes, Bottles, and Accessories

General Filling and Sealing or Capping Requirements

1-mL Syringe
20–22
Gauge Needle
2–3 mm
Buffer
Gradient
General Filling and Sealing or Capping Requirements
See Table 3.1
J series rotors. Maximum fill volume includes sample and gradient. Refer to individual rotor
manuals for specific filling and capping requirements.
3-2
45–50°
Gradient
2–3 mm
Sample
with
2 to 3%
Sucrose
Added
for general filling and sealing or capping requirements for tubes and bottles used in
You can also prepare preformed step gradients by hand,
using a pipette. Carefully layer solutions of decreasing
concentration by placing the tip of the pipette at the
angle formed by the tube wall and the meniscus, or float
the lighter gradient concentrations up by adding
increased density solutions to the tube bottom using a
hypodermic syringe with a long needle such as a
pipetting needle.
Another way to form a linear gradient is to allow a step
gradient to diffuse to linearity. Depending on the
concentration differential between steps and the cross-
sectional area, allow 3 to 6 hours for diffusion at room
temperature, and about 16 hours at 0 to 4°C. For
diffusion of step gradient in Quick-Seal and capped
straightwall tubes, slowly lay the tube on its side (tube
contents will not spill, but make sure the tube does not
roll). After 2 hours at room temperature, slowly set the
tube upright.
Once the gradient is prepared, layer the sample on top
of the gradient.
For thinwall tubes only partially filled with gradient,
add a buffer solution to fill the tube to provide tube wall
support. Although the gradient volume is reduced,
sample volume is not changed.
NOTE If a partially filled thickwall tube is centrifuged,
the tube does not require liquid support, and
therefore, the buffer solution is not required.
PN JR-IM-10AG