The door error message on a Beckman Coulter Avanti J Series centrifuge is likely caused by a door latch issue. You can try the following steps to resolve it:

1. Check the Operator’s Manual – It contains instructions on how to manually unlock the door.
2. Press Down on the Door – Sometimes, pushing down hard on the door can reseat the eyebolts in the latches.
3. Manually Unlock the Door – Follow the manual’s procedure to open the door if it remains locked.
4. Seek On-Site Service – If the latches have failed, professional service may be required.

If these steps do not resolve the issue, further inspection and possible repair may be needed.

This answer is automatically generated

Chaib

February 19, 2025

Ou trouver le schema electrique du verrouillage dela porte de la J6 MC .la centrifugeuse verrouille puis deverrouille juste après [start] .et signale la porte

Summary of Contents for Beckman Coulter Avanti J Series

Page 1 Instructions For Use Rotors and Tubes For Beckman Coulter J2, J6, and Avanti J Series Centrifuges PN JR-IM-10AG October 2014 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
Page 2 Rotors and Tubes For J2, J6, and Avanti J Series Centrifuges PN JR-IM-10AG (October 2014) © 2014 Beckman Coulter, Inc. All rights reserved No part of this document may be reproduced or transmitted in any form or by any means,...
Page 3: Revision History
Revision AE, December 2013 Added the JA-14.50 rotor: See Table 1.1, Rotors Used in Beckman Coulter J Series Centrifuges Table 4.1, General Specifications for Beckman Coulter J Series Fixed-Angle Rotors.
Page 4 Revision History PN JR-IM-10AG...
Page 5: Safety Notice
Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.
Page 6: Mechanical Safety
Beckman Coulter offers the Field Rotor Inspection Program (FRIP). This program involves a visit to your laboratory by a specially trained Beckman Coulter representative, who will inspect all of your rotors for corrosion or damage.
Page 7: Rohs Notice
Safety Notice RoHS Notice RoHS Notice These labels and materials declaration table (the Table of Hazardous Substance’s Name and Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products” requirements. China RoHS Caution Label —...
Page 8 Safety Notice RoHS Notice PN JR-IM-10AG viii...
Page 9: Table Of Contents
Contents Revision History, iii Safety Notice, v Alerts for Warning, Caution, Important, and Note, v Chemical and Biological Safety, v Mechanical Safety, vi RoHS Notice, vii Scope, xvii Scope of this Manual, xvii Rotors, 1-1 Introduction, 1-1 General Description, 1-1 Rotor Designations, 1-1 Materials, 1-3...
Page 10 Contents Tubes, Bottles, and Accessories, 2-1 Introduction, 2-1 Labware Selection Criteria , 2-1 Labware Material Compatibility with Solvents and Sample, 2-2 Gradient Formation and Fractionation, 2-3 Labware Types, 2-3 Polypropylene Tubes, 2-3 Open-Top Polypropylene Tubes, 2-3 Quick-Seal Polypropylene Tubes, 2-4 Polycarbonate Tubes, 2-4 Polyethylene...
Page 11 Contents Polypropylene, 3-6 Polyethylene, 3-6 Stainless Steel, 3-6 Capping Tubes, 3-6 Filling and Capping Tubes, 3-7 Three-Piece Assemblies, 3-7 JLA-8.1000 and JLA-9.1000 Bottle Cap/Closure, 3-7 Filling and Loading Cups in the JS-5.0 Rotor, 3-8 Filling and Sealing Quick-Seal Tubes, 3-9 Capping Multiwell Titer Plates, 3-14 Strips, 3-14...
Page 12 Contents Rotor Preparation, 5-5 Special Preparation Instructions for JS-24 Series Rotors, 5-6 Loading the Rotor Yoke, 5-7 Loading JS-24 Series Rotors, 5-8 Symmetric and Balanced Loading, 5-9 Loading Buckets, 5-11 Using Modular Disk Adapters, 5-11 Using Blood Bag Cups, 5-12 Loading Buckets Into the Rotor, 5-13 Using Microtiter Plate...
Page 13 Disinfection, 7-7 Inspection, 7-9 Tube and Bottle Storage, 7-9 Returning a Rotor or Accessory to the Factory, 7-10 Diagnostic Hints, 7-10 Chemical Resistances for Beckman Coulter Centrifugation Products, A-1 List of Chemical Resistances, A-1 Temperature Compensation Tables, B-1 Introduction, B-1 Temperature...
Page 14 Contents Glossary Beckman Coulter, Inc. J Series Rotor Warranty...
Page 15 Illustrations Illustrations Fixed-Angle, Swinging-Bucket, Vertical-Tube, and Rack-Type Rotors, 1-2 Particle Separation in Fixed-Angle, Swinging-Bucket, and Verti- cal-Tube Rotors., 1-10 Sedimentation Coefficients (in Svedberg Units) for Some Com- mon Biological Materials, 1-12 Nomogram for J2 Series Centrifuges, 1-13 Nomogram for J6Series Centrifuges, 1-14 Arranging Tubes Symmetrically in a Fixed-Angle, Vertical- Tube,...
Page 16 Materials, 2-2 General Filling and Sealing Requirements for Tubes and Bottles, 3-3 Aerosolve Tube Rack, 3-18 General Specifications for Beckman Coulter J Series Fixed-Angle Rotors, 4-3 General Specifications for Beckman Coulter J Series Swinging- Bucket Rotors, 5-3 Microplate Carriers Used with J6-Series...
Page 17 This manual contains general information for properly preparing a rotor for centrifugation in a Beckman Coulter J series centrifuge. This manual should be used with the individual rotor instruction manual shipped with each rotor. The rotor manuals provide specific information for each rotor, including special operating procedures and precautions, tube, bottle, and adapter part numbers, and equations to calculate maximum allowable rotor speeds.
Page 18 • Several appendixes contain information that may be of special interest: — APPENDIX A lists chemical resistances for rotor and accessory materials to help determine compatibility with a variety of solutions. — APPENDIX B contains Temperature Compensation Tables for various rotors. —...
Page 19: Rotors
General Description Rotor Designations Beckman Coulter J series rotors are usually named according to the type of rotor and the rotor’s maximum allowable revolutions per minute (in thousands), referred to as rated speed. For example, the JA-12 rotor is a fixed-angle rotor with a maximum speed of 12,000 rpm. However, the naming system for J series rotors was changed slightly in early 1994.
Page 20: Fixed-Angle, Swinging-Bucket, Vertical-Tube, And Rack-Type Rotors
Rotors General Description Figure 1.1 Fixed-Angle, Swinging-Bucket, Vertical-Tube, and Rack-Type Rotors Fixed-Angle Rotor Swinging-Bucket Rotor Vertical-Tube Rotor Rack-Type Rotor Containers in fixed-angle rotors (designated JA) are held at an angle to the axis of rotation in tube cavities. Containers in J-Lite fixed-angle rotors (designated JLA) are also held at an angle to the axis of rotation; the rotor construction results in reduced overall weight.
Page 21: Materials
General Description Materials Most Beckman Coulter J series rotors are made of aluminum and are anodized to protect the metal from corrosion. (The JS-13.1 and JS-7.5 rotors are painted with polyurethane paint and are not anodized.) The anodized coating is a thin, hard layer of aluminum oxide formed electrochemically in the final stages of rotor fabrication.
Page 22: Drive Pins
Most drive pins are oriented horizontally (or angled) in the drive hole; however, some are oriented vertically. All rotors used in Avanti J series centrifuges must have drive pins in the rotor drive hole. Some Beckman Coulter rotors, including the JA-10 and the JS-7.5, were...
Page 23: Rotors Used In Beckman Coulter J Series Centrifuges
Table 1.1 Rotors Used in Beckman Coulter J Series Centrifuges Nominal Rotor Speed Rotor Capacity (rpm) (x g) k Factor Fixed Angle JA-30.50 Ti 400 mL 30,000 108,860 JA-25.50 400 mL 25,000 76,600 JA-25.15 360 mL 25,000 74,200 (outer row)
Page 24 Table 1.1 Rotors Used in Beckman Coulter J Series Centrifuges (Continued) Nominal Rotor Speed Rotor Capacity (rpm) (x g) k Factor JLA-16.250 1.5 liter 38,400 1090 16,000 JA-14 1.5 liter 14,000 30,100 1764 F14BCI- 700 mL 14,000 33,500 14x50cy F14BCI-6x250y 1500 mL...
Page 25 Table 1.1 Rotors Used in Beckman Coulter J Series Centrifuges (Continued) Nominal Rotor Speed Rotor Capacity (rpm) (x g) k Factor JS-5.9 384 mL 5,900 6,570 JS-5.3 691 mL 5,300 6,130 JS-5.2 4 liters 5,200 6,840 9051 JS-5.0 9 liters...
Page 26 Maximum speed in an Avanti J-E for the rotor with magnets; without magnets maximum is 13,000 rpm. (Maximum speed at 2° C in a 50-Hz centrifuge is 15,000 rpm.) f. Maximum speed in an Avanti J series centrifuge. Maximum speed in a J2 series centrifuge is 14,000 rpm.
Page 27 Rotors Rotor Selection Selection of a rotor depends on a variety of factors, such as sample volume, number of sample components to be separated, particle size, run time, required quality of separation, type of separation, and the centrifuge in use. Fixed-angle, swinging-bucket, vertical-tube, and rack-type rotors are designed to provide optimal separations for a variety of sample types.
Page 28: Particle Separation In Fixed-Angle, Swinging-Bucket, And Verti
Rotors Rotor Selection Figure 1.2 Particle Separation in Fixed-Angle, Swinging-Bucket, and Vertical-Tube Rotors. At Speed At Rest in Rotor At Rest Outside Rotor Fixed-Angle Rotors Pathlength Swinging-Bucket Rotors Pathlength Vertical-Tube Rotors Pathlength * Dark gray represents pelleted material, light gray is floating components, and bands are indicated by black lines. 1-10 PN JR-IM-10AG...
Page 29: Pelleting (Differential Separation)
Figure 1.3 lists sedimentation coefficients for some common biological materials. The k factors at rated speeds for Beckman Coulter J series rotors are provided in the table of general specifications in each rotor use section. * s = dr/dt x l/ω...
Page 30: Sedimentation Coefficients (In Svedberg Units) For Some Com
Figure 1.5 allow you to determine relative centrifugal field (RCF) for a given radius and rotor speed. In Avanti J series centrifuges, the RCF is calculated automatically by the centrifuge software. Run times can be shortened by using partially filled thickwall polypropylene and polycarbonate tubes.
Page 31: Nomogram For J2 Series Centrifuges
Rotors Rotor Selection Figure 1.4 Nomogram for J2 Series Centrifuges 21 000 90 000 20 000 50 000 15 000 10 000 10 000 5 000 5 000 1 000 2 000 1 000 Relative Centrifugal Field Radial Distance Speed (×...
Page 32: Nomogram For J6Series Centrifuges
Rotors Rotor Selection Figure 1.5 Nomogram for J6Series Centrifuges 15 000 6 000 10 000 5 000 5 000 4 000 3 000 2 000 1 000 2 000 1 000 Relative Centrifugal Field Radial Distance Speed (× g) (mm) (rpm) * Align a straightedge through known values in two columns;...
Page 33: Isopycnic Separations
Rotors Rotor Selection Isopycnic Separations A sedimentation-equilibrium, or isopycnic, method separates particles on the basis of particle buoyant density. Each component in the sample travels through the gradient until it reaches an equilibrium position. Particle velocity due to differences in density is given in the following expression: ρ...
Page 34: Blood Component Separations
Rotors General Operating Information Blood Component Separations Centrifugation is the primary method for processing blood because it provides the required high throughput, reproducibility, and versatility. Most blood components can be separated in one or two runs. Generally, two types of runs are performed. •...
Page 35: Rotor Tie-Down
Rotors General Operating Information Figure 1.6 Arranging Tubes Symmetrically in a Fixed-Angle, Vertical-Tube, or JS-24 Series Swinging-Bucket Rotor Rotor Tie-Down Knob To secure the rotor to the drive spindle hub during centrifugation, J series rotors are equipped with devices that screw into the hub. If the rotor is Tie-down left in the centrifuge between runs, tighten the tie-down device before Assembly...
Page 36: Overspeed Protection
At greater speeds, or at rated speeds with heavier loads, rotors are subject to failure. • In Avanti J series centrifuges, an electronic recognition system identifies the rotor, thereby limiting speed to the rated speed of the rotor.
Page 37: Temperature Compensation
Follow the instructions below for the model of centrifuge being used. Avanti J Centrifuges Avanti J series centrifuges provide automatic temperature compensation. Enter the run temperature according to the instructions in your centrifuge instruction manual. No additional input is required.
Page 38: Avanti J2 Series Centrifuges (No Longer Manufactured)
Rotors General Operating Information Avanti J2 Series Centrifuges (No longer manufactured) Set temperature compensation in analog J2 model centrifuges (models J2-HS, J2-21, and J2-HC) as follows. Turn the knob on the centrifuge panel to the required sample temperature. Find the compensation value in APPENDIX B (or in the applicable rotor manual) that corresponds with the required temperature and run speed.
Page 39: Microprocessor-Controlled Centrifuges
Rotors General Operating Information Microprocessor-Controlled Centrifuges Operating temperatures for most rotors are contained in memory in microprocessor-controlled centrifuges (model J6-MI, and discontinued models J6-MC, J2-MI, J2-21M, J2-MC, and J2-21M/E). Set temperature compensation as follows for rotors not in centrifuge memory. Press the key on the centrifuge control panel and then use the keypad to enter the TEMP...
Page 40 Rotors General Operating Information 1-22 PN JR-IM-10AG...
Page 41: Tubes, Bottles, And Accessories
* A complete list of tubes, bottles, and accessories is provided in the latest edition of the Beckman Coulter High Performance, High Speed, High Capacity Rotors, Tubes, and Accessories catalog (BR-8102), available at www.beckmancoulter.com.
Page 42: Labware Material Compatibility With Solvents And Sample
Tubes, Bottles, and Accessories Labware Selection Criteria Table 2.1 Characteristics and Chemical Resistances of Tube and Bottle Materials thinwall transparent polypropylene thickwall translucent polypropylene Ultra-Clear transparent polycarbonate transparent polypropylene translucent/ transparent polyethylene transparent/ translucent cellulose transparent propionate stainless steel opaque S = satisfactory resistance M = marginal resistance U = unsatisfactory resistance...
Page 43: Gradient Formation And Fractionation
Tubes made of cellulose nitrate were formerly popular for various separations, particularly rate-zonal separations. Beckman Coulter discontinued the use of cellulose nitrate for tube manufacture in 1980, due to inconsistent physical properties inherent in the material. If you currently have cellulose nitrate tubes, dispose of them.
Page 44: Quick-Seal Polypropylene Tubes
Tubes, Bottles, and Accessories Labware Types Quick-Seal Polypropylene Tubes Heat-sealed Quick-Seal tubes can be used in some fixed-angle rotors g-Max and in the JS-24 series rotors; they must be used in the JV-20 vertical- Metal Floating tube rotor. Single-use Quick-Seal tubes are a convenient form of Spacer Spacer sealable tube;...
Page 45: Stainless Steel Tubes
Tubes, Bottles, and Accessories Labware Types Stainless Steel Tubes Stainless steel tubes offer excellent resistance to organic solvents and heat, but should not be used with most acids or bases. They offer only marginal resistance to most gradient-forming materials other than sucrose and glycerol. Stainless steel tubes are very strong and can be centrifuged when filled to any level.
Page 46: Deep-Well Titer Plates (And Caps)
Tubes, Bottles, and Accessories Temperature Limits Deep-Well Titer Plates (and Caps) 96-Cap Strip These plates are manufactured of sterile or nonsterile polystyrene or polypropylene. The plates can contain up to 1.2 mL per well of sample and solvent in a single 96-well plate when run uncapped.
Page 47: Spacers And Floating Spacers
To prevent the bottles from stretching or breaking, a plastic sleeve, or adapter, must be used around each Beckman Coulter 1-liter bottle during centrifugation Adapter in J6 series rotors. In other rotors, if the bottles fit snugly in the buckets, the adapters are not required.
Page 48: Solid Multitube Adapters
JS-4.3 decanted at once. Do not intermix modular adapters (or their individual parts) from Beckman Coulter’s J6 series rotors with those Bails for the JS-4.3 rotor. While the adapters are similar in appearance, they have very different weights. J6 adapters have bails (vertical supports) that are curved at the top;...
Page 49: Bottle And Tube Caps
Tubes, Bottles, and Accessories Bottle and Tube Caps Bottle and Tube Caps The need for caps depends on such factors as the kind of rotor being used, the type of container, and the amount of sample being centrifuged. Some tubes must be capped before centrifugation, as in the case of thinwall tubes. The thickness and strength of some containers, such as thickwall plastic and stainless steel tubes used in fixed- angle and swinging-bucket rotors, allows them to be run without caps, but they must be only partially filled.
Page 50: Blood Bag Cups
Tubes, Bottles, and Accessories Blood Bag Cups Blood Bag Cups Polypropylene blood bag cups are available for use in swinging-bucket rotors to obtain cell-free plasma for cell packing or for leukolyte depletion. Different sizes of cups are available to accommodate single, double, triple, or quad pack blood bags.
Page 51: Tubes, Bottles, And Accessories
Added Last * A complete list of tubes, bottles, and adapters is provided in the latest edition of the Beckman Coulter High Performance, High Speed, High Capacity Rotors, Tubes & Accessories catalog (BR-8102), available at www.beckmancoulter.com. PN JR-IM-10AG...
Page 52: General Filling And Sealing Or Capping Requirements
Tubes, Bottles, and Accessories General Filling and Sealing or Capping Requirements You can also prepare preformed step gradients by hand, using a pipette. Carefully layer solutions of decreasing concentration by placing the tip of the pipette at the angle formed by the tube wall and the meniscus, or float the lighter gradient concentrations up by adding increased density solutions to the tube bottom using a hypodermic syringe with a long needle such as a...
Page 53: General Filling And Sealing Requirements For Tubes And
Tubes, Bottles, and Accessories General Filling and Sealing or Capping Requirements Table 3.1 General Filling and Sealing Requirements for Tubes and Bottles Filling Level Requirements Vertical-Tube Tube or Bottle Swinging-Bucket Rotors Fixed-Angle Rotors Rotors Polypropylene thinwall tubes within 2 to 3 mm of top full with cap not used thickwall tubes...
Page 54: Working With Physiological Fluids
Three levels of containment are offered by Beckman Coulter, and may be used singly or combined, depending upon your application. 1. Capped tubes or bottles are designed to provide fluid containment. We strongly recommend that all containers carrying physiological fluids be capped to prevent leakage.
Page 55: Filling Open-Top Tubes
Tubes, Bottles, and Accessories Filling Open-Top Tubes Filling Open-Top Tubes Open-Top Polypropylene Tubes Open-top polypropylene tubes are used in swinging bucket and fixed-angle rotors. Swinging-Bucket Rotors Fill all opposing tubes to the same level. • Thinwall Tubes—Fill to within 2 or 3 mm of the top for proper tube wall support.
Page 56: Polypropylene
Tubes, Bottles, and Accessories Capping Tubes Polypropylene Fill all opposing tubes to the same level. • For swinging-bucket rotors, fill to within 2 or 3 mm of the top of the tube. • Fill thickwall polypropylene tubes at least half full to maximum level in fixed-angle rotors. Speed reduction is required.
Page 57: Filling And Capping Tubes
Tubes, Bottles, and Accessories Filling and Capping Tubes Filling and Capping Tubes To prevent spillage and provide support, polycarbonate and polypropylene bottles used in fixed- angle rotors must be capped when fill levels exceed the maximum level allowed for uncapped bottles.
Page 58: Filling And Loading Cups In The
Tubes, Bottles, and Accessories Filling and Loading Cups in the JS-5.0 Rotor Filling and Loading Cups in the JS-5.0 Rotor NOTE cups must be used for every run and must be balanced to within 25 grams of Four labware each other. Do not load the rotor with two filled cups and two empty cups Insert four labware cups into two cup racks with the cup latch hinges toward the center of the racks.
Page 59: Filling And Sealing Quick-Seal Tubes
(no longer available). Refer to How to Use Quick-Seal Tubes with the Beckman Coulter Cordless Tube Topper (publication IN-181) for detailed information about the Tube Topper. Instructions for using the older tabletop tube sealer are in How to Use Quick-Seal Tubes with the Beckman Tube Sealer (publication IN-163).
Page 60: The Cordless Quick-Seal Tube Topper
Tubes, Bottles, and Accessories Filling and Sealing Quick-Seal Tubes Figure 3.1 The Cordless Quick-Seal Tube Topper Charging Stand Pushbutton CAUTION Before plugging in the Tube Topper, be sure that you have a proper power source (120 V, 50 or 60 Hz). Charge your Cordless Tube Topper only in the charging stand supplied with it.
Page 61 Tubes, Bottles, and Accessories Filling and Sealing Quick-Seal Tubes Seal each tube using Method A (With the Seal Guide) or Method B (Without the Seal Guide). Method A is preferable when sealing smaller tubes or when resealing a tube that leaks. CAUTION Always keep the Tube Topper in its charging stand when not in use.
Page 62 Tubes, Bottles, and Accessories Filling and Sealing Quick-Seal Tubes Heat Sink When the seal guide has moved to the correct position, remove the Tube Topper and pinch the circular seal guide to hold the seal former in place. Small Place the heat sink (small end) over the cap for 2 to 3 seconds while the plastic cools—do NOT let the seal former Removal Tool pop up.
Page 63 Tubes, Bottles, and Accessories Filling and Sealing Quick-Seal Tubes Immediately NOTE It is very important to apply the heat sink immediately. To do so, we recommend that you have it in one hand, ready Heat Sink to apply as soon as needed. Large Remove the Tube Topper.
Page 64: Capping Multiwell Titer Plates
Tubes, Bottles, and Accessories Filling and Sealing Quick-Seal Tubes Capping Multiwell Titer Plates Multiwell titer plates—regular, deep-well, and square-well—can be run uncovered or using one of the available cover types. Cap Strips Available sterile or nonsterile 96-cap strips can be used with deep-well plates. (When these caps are used, the capacity of each well is reduced to 1.0 mL.) 96-Cap Strip Aluminum Foil Lids...
Page 65: Using Modular Disk Multitube Adapters
Tubes, Bottles, and Accessories Filling and Sealing Quick-Seal Tubes Using Modular Disk Multitube Adapters These adapters can also be used as tube racks in the laboratory. The adapter disks are color-coded by the tube size they accommodate; the number of disks used in an adapter assembly depends upon the length of tubes used.
Page 66: Using Aerosolve Canisters
Tubes, Bottles, and Accessories Using Aerosolve Canisters Using Aerosolve Canisters Aerosolve canisters can be used in the JS-4.3 rotor to minimize aerosol leakage and liquid spills from rotor buckets during centrifugation. Each canister can hold a variety of tube sizes in tube racks that are specifically designed to fit in the canisters.
Page 67: Using Canisters As Wide-Mouth Bottles
Tubes, Bottles, and Accessories Using Aerosolve Canisters Fill the canister as described under Using Canisters as Wide-Mouth Bottles, or Using Canisters with Tube Racks, below. Screw the lid on until closing resistance is first felt, then tighten it an additional 60 degrees. The scribe marks around the rim of the canister and the corrugated finger grips on the lid are all placed 60 degrees apart.
Page 68: Using Canisters With Tube Racks
Tubes, Bottles, and Accessories Using Aerosolve Canisters Using Canisters with Tube Racks The racks designed to hold tubes in the Aerosolve canister are listed in Table 3.2. Tube racks are easily disassembled by unscrewing the handle and lifting off the top plate. Press a rubber cushion (if applicable—see Table 3.2) into each tube hole in the rack base.
Page 69: Using Blood Bag Cups
Tubes, Bottles, and Accessories Using Blood Bag Cups Table 3.2 Aerosolve Tube Rack (Continued) Nominal Nominal Maximum Part Number Tube Tube Tube Number Cushion Volume Diameter Tubes per (set of (set of Part (mL) (mm) Adapter four racks) two racks) Rack Color Number light green...
Page 70: Sample Recovery
Tubes, Bottles, and Accessories Sample Recovery Sandwich the tubing between the blood bag and any transfer packs. Ports Sandwich Coiled Tubing between Transfer Bags Blood and Filled Blood Bag Seam Blood Label Blood Bag Cup Make sure the loaded blood bag cups opposite each other on the rotor yoke are approximately the same weight (within 1 gram).
Page 71: Capped Tubes
Do not use a metal tool to remove pellet, as metal could damage the cup and shorten its useful life. * Beckman Coulter recommends Cole-Parmer heat sealer Model U-03018-10, adjusted to setting 3 or 4. Contact Cole- Parmer at (800) 323-4340, by Fax at (847) 247-2929, or at www.coleparmer.com.
Page 72: Quick-Seal Tubes
CentriTube Slicer. CentriTube Slicer (347960) For additional information on fraction recovery systems available from Beckman Coulter, refer to the latest edition of High Performance, High Speed, High Capacity Rotors, Tubes & Accessories (publication BR-8102) available at www.beckmancoulter.com.
Page 73: Making Ultra-Clear Tubes Wettable
Tubes, Bottles, and Accessories Making Ultra-Clear Tubes Wettable Making Ultra-Clear Tubes Wettable The following method of making Ultra-Clear tubes wettable has proven successful for some users: 1. Polyvinyl alcohol (2 g) was dissolved in distilled water (50 mL) by stirring and heating to gentle reflux.
Page 74 Tubes, Bottles, and Accessories Making Ultra-Clear Tubes Wettable 3-24 PN JR-IM-10AG...
Page 75: Using Fixed-Angle Rotors
CHAPTER 4 Using Fixed-Angle Rotors Introduction This section contains instructions for using fixed-angle rotors in J series centrifuges. In addition to these instructions, observe procedures and precautions provided in the applicable rotor and centrifuge manuals. Refer to CHAPTER 2 for labware selection information, and CHAPTER 3 for recommended filling and sealing or capping requirements and for sample recovery procedures.
Page 76: Examples Of Fixed-Angle Rotors
Using Fixed-Angle Rotors Description Figure 4.1 Examples of Fixed-Angle Rotors 34° JA-20 Axis of Rotation 25° Axis of Rotation JA-25.15 20° JLA-10.500 Axis of Rotation PN JR-IM-10AG...
Page 77: General Specifications For Beckman Coulter J Series Fixed-Angle Rotors
Using Fixed-Angle Rotors Description Table 4.1 General Specifications for Beckman Coulter J Series Fixed-Angle Rotors Critical Radial Distances (mm) Number of Speed × Speed Tubes Nominal Nominal RCF/ Range Capacity of Rotor Rotor Profile and Name k Factor (rpm) Largest Tube Capacity ×...
Page 78 Using Fixed-Angle Rotors Description Table 4.1 General Specifications for Beckman Coulter J Series Fixed-Angle Rotors (Continued) Critical Radial Distances (mm) Number of Speed × Speed Tubes Nominal Nominal RCF/ Range Capacity of Rotor Rotor Profile and Name k Factor (rpm)
Page 79 15,000 rpm; when 25° adapters are used, do not run the rotor above 16,000 rpm. e. Maximum speed in an Avanti J series centrifuge, except Avanti J-E; maximum speed in an Avanti J-E for a rotor with magnets, maximum speed for rotor without magnets is 13,000 rpm.
Page 80: Tubes And Bottles
Although rotor components and accessories made by other manufacturers may fit in the Beckman Coulter rotor you are using, their safety in the rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the Beckman Coulter rotor may void the rotor warranty, and should be prohibited by your laboratory safety officer.
Page 81: Rotor Preparation And Loading
Using Fixed-Angle Rotors Rotor Preparation and Loading Rotor Preparation and Loading For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. Prerun Safety Check Read all safety information in the rotor manual before using the rotor. Make sure that the rotor and lid are clean and show no signs of corrosion or cracking.
Page 82: Operation
Refer to APPENDIX B or to the applicable rotor manual for tables listing temperature compensation units for various rotors.) • If you are using an Avanti J series centrifuge (except J-E), select the rotor number. Rotor : JA-25.50 Speed : 0...
Page 83: Installing The Rotor
Using Fixed-Angle Rotors Operation Installing the Rotor CAUTION The centrifuge drive spindle can be bent or broken if the rotor is forced sideways or dropped onto it. Install the rotor by centering it over the spindle and carefully lowering it straight down. Carefully lower the rotor straight down onto the drive spindle.
Page 84: Removal And Sample Recovery
NOTE The JA-18 rotor must be run with the lid on in Avanti J series centrifuges. Removal and Sample Recovery CAUTION If disassembly reveals evidence of leakage, you should assume that some fluid escaped the container or rotor.
Page 85 Lubrication of the centrifuge drive spindle hub with Spinkote should prevent the rotor from sticking on all centrifuges except Avanti J series. Avanti J series centrifuges have acetal rings on the spindle hubs to prevent sticking and do not require lubrication.
Page 86 Using Fixed-Angle Rotors Operation 4-12 PN JR-IM-10AG...
Page 87: Using Swinging-Bucket Rotors
NOTE Although rotor components and accessories made by other manufacturers may fit in the Beckman Coulter rotor you are using, their safety in the rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the rotor may void the rotor warranty, and should be prohibited by your laboratory safety officer.
Page 88: Examples Of Swinging-Bucket Rotors
Using Swinging-Bucket Rotors Description Figure 5.1 Examples of Swinging-Bucket Rotors JS-7.5 Axis of Rotation Axis of Rotation JS-4.3 JS-13.1 Axis of Rotation PN JR-IM-10AG...
Page 89: General Specifications For Beckman Coulter J Series Swinging
Using Swinging-Bucket Rotors Description Table 5.1 General Specifications for Beckman Coulter J Series Swinging-Bucket Rotors Critical Radial Distances (mm) Number of Speed Max Speed Tubes x Nominal Nominal RCF/ Range Capacity of Rotor Rotor Profile and Name k Factor (rpm)
Page 90: Labware
Using Swinging-Bucket Rotors Description Table 5.1 General Specifications for Beckman Coulter J Series Swinging-Bucket Rotors (Continued) Critical Radial Distances (mm) Number of Speed Max Speed Tubes x Nominal Nominal RCF/ Range Capacity of Rotor Rotor Profile and Name k Factor...
Page 91: Rotor Preparation And Loading
Verify that the tubes, bottles, or carriers being used are listed in the applicable rotor manual. If fluid containment is required, use capped tubes or bottles and/or Aerosolve canisters. Beckman Coulter strongly recommends capping all containers carrying physiological fluids to prevent leakage.
Page 92: Special Preparation Instructions For Js-24 Series Rotors
Using Swinging-Bucket Rotors Rotor Preparation and Loading Before each use of the rotor, make sure that bucket pin sockets are lubricated with Paint On Graphite Lubricant (977212) as described in CHAPTER Lubrication Points Rotor Yoke (both pin sockets) Special Preparation Instructions for JS-24 Series Rotors Place the rotor on the rotor stand (362785) when it is not in the centrifuge.
Page 93: Loading The Rotor Yoke
Using Swinging-Bucket Rotors Rotor Preparation and Loading Loading the Rotor Yoke CAUTION The centrifuge drive spindle can be bent or broken if the rotor is forced sideways or dropped onto it. Install the rotor by centering it over the spindle and carefully lowering it straight down.
Page 94: Loading Js-24 Series Rotors
Using Swinging-Bucket Rotors Rotor Preparation and Loading c. JS-4.2A and JS-4.2SMA—secure the rotor to the drive hub with the tie-down bolt (368518). Tighten the bolt with the tie-down tool (368521), then remove the tool. Tie-down Tool (368521)) Tie-down Bolt (368518) NOTE Older JS-4.2A or JS-4.2SMA rotors may be secured to the drive hub with tie down bolt (339031).
Page 95: Symmetric And Balanced Loading
100 grams unbalanced due to different loading volumes or tube or bag breakage. Beckman Coulter supplies buckets and carriers for most rotors in weight-matched sets to make balancing easier (the weight and date of manufacture are marked on the side of each bucket and bottom of each carrier).
Page 96: Examples Of Correctly And Incorrectly Loaded Buckets And Carriers
Using Swinging-Bucket Rotors Rotor Preparation and Loading Figure 5.2 Examples of Correctly and Incorrectly Loaded Buckets and Carriers Balanced Load Unbalanced Load Pivotal Axis of Bucket Center of Rotation Correct Incorrect Examples of Symmetrically Loaded Trays Examples of Nonsymmetrically (Load Opposite Trays the Same Way) Loaded Trays Pivotal Axis Carrier...
Page 97: Loading Buckets
Using Swinging-Bucket Rotors Rotor Preparation and Loading Loading Buckets Buckets can be loaded before or after being installed on the rotor yoke. For best results, fill the appropriate labware first and then load the labware into the buckets. This is especially important when using blood bags—you can trip the imbalance detector in the centrifuge by pushing blood bags into cups within buckets that are installed in the rotor.
Page 98: Using Blood Bag Cups
Using Swinging-Bucket Rotors Rotor Preparation and Loading Using Blood Bag Cups Do not pour liquid directly into blood bag cups. Fit blood bags into cups before loading the cups into the rotor buckets. Load the blood bag cups as follows: Load the cups so that the blood bags and tubing fit as far down as possible.
Page 99: Loading Buckets Into The Rotor
Using Swinging-Bucket Rotors Rotor Preparation and Loading Figure 5.3 Typical Blood Bag Loading Procedures (JS-24.3 Rotor Shown) Sandwich Coiled Blood Bag Label Tubing between Ports Blood Bag Seam Transfer Bags Aligned with and Filled Pivotal Axis of Blood Bag Bucket Blood Bag Seam Blood Bag...
Page 100: Using Microtiter Plate Carriers
Using Swinging-Bucket Rotors Rotor Preparation and Loading Load the filled buckets (and/or carriers) onto the rotor yoke pivot pins, following the instructions in the rotor manual. Make sure that the buckets are properly seated by gently swinging them on the pivot pins. NOTE All positions on the rotor yoke must contain either a bucket or a microtiter plate carrier during a run.
Page 101: Js-5.9 And Js-5.3 Plate Carriers
Using Swinging-Bucket Rotors Rotor Preparation and Loading Figure 5.4 The Micro Plus Microtiter Plate Carrier, Base, Pad, and Deep-Well Microtiter Plate Deep-well Plate Carrier Base JS-5.9 and JS-5.3 Plate Carriers High-impact thermoplastic carriers are used in the JS-5.9 Carrier JS-5.9 and JS-5.3 rotor buckets to provide support to labware during centrifugation and facilitate loading Support Pad and unloading buckets.
Page 102: J6 Carriers
Using Swinging-Bucket Rotors Rotor Preparation and Loading If using polystyrene or stacked polypropylene plates, place a support pad in the carrier with the ridged-cross side down. After centrifugation, grasp the carrier by the wire handles and lift it straight up out of the bucket to unload it.
Page 103: Operation
Using Swinging-Bucket Rotors Operation Operation Refer to the centrifuge instruction manual for detailed operating information. For low-temperature runs, precool the rotor in the centrifuge or in a refrigerator before use—especially before short runs—to ensure that the rotor reaches the set temperature. (To ensure that the rotor reaches the required temperature during centrifugation, some temperature compensation may be required because of the mass of these rotors.
Page 104 Using Swinging-Bucket Rotors Sample Recovery Remove the bucket caps and use the appropriate removal tool (listed in the rotor manual) to remove the spacers and tubes. If floating spacers were used, remove them with the threaded end of the floating spacer removal tool (338765).
Page 105: Using Vertical-Tube And Rack-Type Rotors
Description Table 6.1 Refer to for general operating specifications for vertical-tube and rack-type rotors. Table 6.1 General Specifications for Beckman Coulter J Series Vertical-Tube and Rack-Type Rotors Radial Distances (mm) Critical Number of Tubes ´ Nominal...
Page 106: Vertical-Tube Rotors
Using Vertical-Tube and Rack-Type Rotors Description Vertical-Tube Rotors Vertical-tube rotors (see Figure 6.1) hold tubes parallel to the axis of rotation; therefore, bands separate across the diameter of the tube rather than down the length of the tube (see Figure 1.3).
Page 107: Using A Vertical-Tube Rotor
Although rotor components and accessories made by other manufacturers may fit in the Beckman Coulter rotor you are using, their safety in the rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the Beckman Coulter rotor may void the rotor warranty, and should be prohibited by your laboratory safety officer.
Page 108: Operation
• If you are using an Avanti J series centrifuge, select the rotor number. • If you are using a microprocessor-controlled J2 or J6 series centrifuge, enter the rotor code (enter code 20 for the JV-20 rotor).
Page 109: Installing The Rotor
Using Vertical-Tube and Rack-Type Rotors Using a Vertical-Tube Rotor Installing the Rotor CAUTION The centrifuge drive spindle can be bent or broken if the rotor is forced sideways or dropped onto it. Install the rotor by centering it over the spindle and carefully lowering it straight down.
Page 110: Removal And Sample Recovery
Lubrication of the centrifuge drive spindle hub with Spinkote should prevent the rotor from sticking on all centrifuges except Avanti J series. Avanti J series centrifuges have acetal rings on the spindle hubs to prevent sticking and do not require lubrication.
Page 111: Using A Rack-Type Rotor
Using Vertical-Tube and Rack-Type Rotors Using a Rack-Type Rotor Using a Rack-Type Rotor Trays and Tubes Two kinds of trays are available for use in the rack-type rotor to accommodate a variety of racks and tube sizes. The trays can be identified by color, as listed in the rotor manual. Some racks require the use of adapters, spacers, or frames to ensure a proper fit in the tray.
Page 112: Operation
If you are using a microprocessor-controlled J6 series centrifuge, enter the rotor code (enter code 3.2 for the JR-3.2 rotor). NOTE These rotors are not used in Avanti J series centrifuges. PN JR-IM-10AG...
Page 113: Installing The Rotor
Using Vertical-Tube and Rack-Type Rotors Using a Rack-Type Rotor Installing the Rotor CAUTION The centrifuge drive spindle can be bent or broken if the rotor is forced sideways or dropped onto it. Install the rotor by centering it over the spindle and carefully lowering it straight down.
Page 114: Removal And Sample Recovery
Using Vertical-Tube and Rack-Type Rotors Using a Rack-Type Rotor Removal and Sample Recovery CAUTION If disassembly reveals evidence of leakage, you should assume that some fluid escaped the container or rotor. Apply appropriate decontamination procedures to the centrifuge, rotor, and accessories. Turn the tie-down knob to the left (counterclockwise) to release the rotor from the drive spindle.
Page 115: Care And Maintenance
CHAPTER 7 Care and Maintenance Introduction This section provides information on the care of rotors and accessories. Included is a list of some common operating problems with suggestions for their solutions. Rotors and accessories should be kept in optimal condition, thus minimizing the chances of rotor or labware failure. In addition to these instructions, observe procedures and precautions provided in individual rotor manuals.
Page 116: Cleaning
Care and Maintenance Rotor Care Cleaning Wash rotors and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred. DO NOT allow corrosive materials to dry on the rotor. NOTE Do not wash rotor components or accessories in a dishwasher. Do not soak in detergent solution for long periods, such as overnight.
Page 117: Decontamination
IsoClean usage instructions. Then remove it promptly from surfaces. While Beckman Coulter has tested these methods and found that they do not damage components, no guarantee of decontamination is expressed or implied. Consult your laboratory safety officer regarding the proper decontamination methods to use.
Page 118: Sterilization And Disinfection
When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use. While Beckman Coulter has tested the following methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied.
Page 119: Lubrication
Aluminum- Composite other damage is visible on the outside wall or near the Interface aluminum-composite interface area, do not use the Outside canister. Contact your Beckman Coulter representative. Sleeve Wall Washer Retire the canister on the expiration date. Lubrication Regular and thorough lubrication can extend the useful life of rotor components.
Page 120: Field Rotor Inspection Program
• Do not dry tubes, bottles, or accessories in an oven. Labware should be air-dried. • Quick-Seal, Ultra-Clear, and thinwall polypropylene tubes are intended for one-time use and should be discarded after use. * Call 1-800-742-2345 (U.S.A. or Canada), outside the U.S., contact your local Beckman Coulter office. PN JR-IM-10AG...
Page 121: Decontamination
Care and Maintenance Tube, Bottle, and Accessory Care Decontamination Labware contaminated with radioactive or pathogenic solutions should be decontaminated or disposed of following appropriate safety guidelines and/or regulations. Consult Chemical Resistances (IN-175) to select an agent that will not damage the tube or bottle material. Sterilization and Disinfection Refer to Table 7.1...
Page 122: Tube And Bottle Sterilization And Disinfection
Care and Maintenance Tube, Bottle, and Accessory Care Table 7.1 Tube and Bottle Sterilization and Disinfection Sodium Hypo- Hydrogen Glutaral- Phenolic Ethanol Formal- Tube/Bottle Autoclave Ethylene chlorite Peroxide dehyde Deriva- dehyde Material (121°C) Irradiation Oxide (70%) (10%) (10%) (2%) tives polypropylene Ultra-Clear polycarbonate...
Page 123: Inspection
Care and Maintenance Tube, Bottle, and Accessory Care While Beckman Coulter has tested these methods and found that they do not damage the components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.
Page 124: Returning A Rotor Or Accessory To The Factory
Some of the more common operating problems experienced in centrifugation are listed in Table 7.2 with suggestions for their solutions. Contact your Beckman Coulter Field Service representative if a problem cannot be corrected. NOTE Use only the labware listed in the applicable rotor manual.
Page 125: Troubleshooting Chart
Do not use excessive force to loosen components. Contact your Beckman Coulter representative. Routinely clean metal threads with concentrated Solution 555 that has been diluted to 10 parts water to 1 part detergent, then lubricate them with Spinkote.
Page 126 Care and Maintenance Diagnostic Hints Table 7.2 Troubleshooting Chart (Continued) Symptom Possible Cause and Suggested Action Tubes with snap-on caps Tube too full; the meniscus must be kept lower to prevent leakage. Uncapped tubes Tube volume exceeds maximum uncapped volume. Refer to the rotor manual for tube volumes and speed reductions.
Page 127 Care and Maintenance Diagnostic Hints Table 7.2 Troubleshooting Chart (Continued) Symptom Possible Cause and Suggested Action Bottle leakage (uncapped bottles) Bottle too full; the meniscus must be kept lower to prevent leakage. Refer to the rotor manual for fill volumes and speed reductions. Bottle damage •...
Page 128 Care and Maintenance Diagnostic Hints 7-14 PN JR-IM-10AG...
Page 129: Chemical Resistances For Beckman Coulter Centrifugation Products
APPENDIX A Chemical Resistances for Beckman Coulter Centrifugation Products List of Chemical Resistances Appendix A is replicated in the separate pdf document, Chemical Resistances (IN-175) PN JR-IM-10AG...
Page 130 Chemical Resistances for Beckman Coulter Centrifugation Products List of Chemical Resistances PN JR-IM-10AG...
Page 131: Temperature Compensation Tables
J centrifuge being used. NOTE When using an Avanti J series centrifuge, enter the run temperature according to the instructions in your centrifuge instruction manual. No additional input is required. Table B.1 Temperature Compensation Settings for theJ2-HC Centrifuge Required Sample temperature (°C, green bar)
Page 132 Temperature Compensation Tables Temperature Compensation Table B.1 Temperature Compensation Settings for theJ2-HC Centrifuge (Continued) Required Sample temperature (°C, green bar) Speed Rotor (rpm) –20°C –10°C 2°C 5°C 10°C 20°C 40°C 18,000 –4 – – – – JA-20 15,000 –3 – –...
Page 133: Temperature Compensation Settings For The J2-21, J2-21B
Temperature Compensation Tables Temperature Compensation Table B.1 Temperature Compensation Settings for theJ2-HC Centrifuge (Continued) Required Sample temperature (°C, green bar) Speed Rotor (rpm) –20°C –10°C 2°C 5°C 10°C 20°C 40°C 4,300 JS-4.3 3,000 1,500 18,000 – – – – – JV-20 15,000 –...
Page 134 Temperature Compensation Tables Temperature Compensation Table B.2 Temperature Compensation Settings for the J2-21, J2-21B, J2-21C, and J2-HS Centrifuges (Continued) Required Sample temperature (°C, green bar) Speed Rotor (rpm) –20°C –10°C 2°C 5°C 10°C 20°C 40°C 18,000 –10 –7 17,000 –10 –9 –8 –6...
Page 135: Temperature Compensation Settings For The J2-Mi, J2-21M, J2-Mc, And J2-21M/E Centrifuges
Temperature Compensation Tables Temperature Compensation Table B.3 Temperature Compensation Settings for the J2-MI, J2-21M, J2-MC, and J2-21M/E Centrifuges Required Sample Temperature (°C, green bar) Speed Rotor (rpm) –20°C –10°C 2°C 5°C 10°C 20°C 40°C 20,000 – – – – – 18,000 –...
Page 136: Temperature Compensation Settings For The J6
Temperature Compensation Tables Temperature Compensation Table B.3 Temperature Compensation Settings for the J2-MI, J2-21M, J2-MC, and J2-21M/E Centrifuges (Continued) Required Sample Temperature (°C, green bar) Speed Rotor (rpm) –20°C –10°C 2°C 5°C 10°C 20°C 40°C 12,000 – – – – –...
Page 137: Gradient Materials
APPENDIX C Gradient Materials Introduction This Appendix contains reference information on commonly used gradient materials. General instructions for filling and sealing tubes, including gradient preparation, are contained in CHAPTER Gradient material selection depends on a number of factors, including the type of separation to be performed.
Page 138: Materials
Gradient Materials Introduction Table C.1 Commonly Used Gradient Materials with Their Solvents Maximum Density Materials Solvent at 20°C Sucrose (66%) 1.32 Sucrose (65%) 1.37 Silica sols 1.30 Diodon 1.37 Glycerol 1.26 Cesium chloride 1.91 1.98 Cesium formate 2.010 Cesium acetate 2.00 Rubidium chloride 1.49...
Page 139: Density, Refractive Index, And Concentration Data-Cesium
Gradient Materials Introduction Table C.2 Density, Refractive Index, and Concentration Data—Cesium Chloride at 25°C, Molecular Weight = 168.37 Density Refractive Density Refractive % by mg/mL of % by mg/mL of Index, ηD Index, ηD (g/cm3) Molarity (g/cm3) Molarity Weight Solution Weight Solution 1.3333...
Page 140: Density, Refractive Index, And Concentration Data-Sucrose At
Gradient Materials Introduction Table C.3 Density, Refractive Index, and Concentration Data—Sucrose at 20°C, Molecular Weight = 342.3 Density Refractive Density Refractive % by mg/mL of % by mg/mL of Index, ηD Index, ηD (g/cm3) Molarity (g/cm3) Molarity Weight Solution Weight Solution 0.9982 1.3330...
Page 141 Gradient Materials Introduction Table C.4 Density Conversion for Cesium and Rubidum Salts at 20°C % w/w CsCl CsBr CsNO3 RbCl RbBr RbNO 1.00593 1.00612 1.00608 1.0061 1.00566 1.00561 1.00593 1.00591 1.0066 1.0053 1.01374 1.01412 1.01402 1.0144 1.01319 1.01307 1.01372 1.01370 1.0150 1.0125 1.02969...
Page 142 Gradient Materials Introduction PN JR-IM-10AG...
Page 143: Blood Component Separation
APPENDIX D Blood Component Separation Introduction This Appendix provides a basic overview of blood separation procedures using Beckman Coulter J series centrifuges. Blood Bank Collection Overview Blood is composed of plasma, red blood cells (RBC), white blood cells (WBC), and platelets.
Page 144: Components And Typical Usage
Blood Component Separation Components and Typical Usage Components and Typical Usage Single-Donor Fresh Plasma Single-donor fresh plasma is separated from whole blood within 4 to 8 hours after collection. If it is not used immediately, it may be frozen and stored (see Shelf Life, below).
Page 145: Shelf Life
Blood Component Separation Shelf Life Shelf Life Table D.1 lists approximate storage times for the separated components. Table D.1 Blood Component Storage Approximate Component Storage Life 42 days RBC with ADSOL 35 days RBC with SAG-M 25 days RBC with CPDA-1 RBC without preservative 21 days RBC frozen without addition of...
Page 146: Blood Component Separation
Antihemophilic Factor (Factor VIII) Beckman Coulter has centrifuges, rotors, and accessories designed to fit the special needs of blood component processing. Several rotors are available to accommodate single, double, triple, and quad blood bags. Blood bag cups rest in the rotor bucket and simplify processing, since they eliminate the need to remove buckets after each run.
Page 147: Blood Bank Methods
Blood Component Separation Separation of Blood Components by Centrifugation Table D.2 Blood Bank Methods Blood Temper- Capacity Brake Setting Components Starting ature (No. of Speed Time J6-MC J6-HC Method Rotor J6-MI Products Material (°C) Cups) (rpm) (min) Platelet-Rich Whole Soft 20 to 22 JS-4.2 2800...
Page 148: Tips For Optimum Centrifugation Runs
Blood Component Separation Tips for Optimum Centrifugation Runs Tips for Optimum Centrifugation Runs Centrifugation generates high speeds, causing rotor heads and buckets to develop a gravity force of thousands of pounds. Observing the following tips will ensure safe and efficient operation. WARNING Handle body fluids with care because they can transmit disease.
Page 149: References
List of References Documents listed below can be obtained by calling Beckman Coulter Customer Service at 1-800-742-2345 (U.S.A. or Canada) or by contacting your local Beckman Coulter office. They are also available at www.beckmancoulter.com. IN-181 How to use Quick-Seal Tubes with the Beckman Coulter Cordless Tube Topper.
Page 150 Avanti J-30I High-Performance Centrifuge and Avanti J-HC High Capacity Bioprocessing Centrifuge Systems DS10009 Avanti J-25 Series High-Performance Centrifuges FL-9987 F10BCI 6x500y FIBERLite Carbon Fiber Rotor for Avanti J Series High-Performance Centrifuges SB-812 J6 Series High-Capacity Centrifuges T-1735 Modification of the Model J6-MC for Blood Component Preparation T-1741 Optimizing Radioimmunoassays with the JR-3.2 Rack Rotor...
Page 151 –57 and 120°C (–70 and 250°F) Clearing factor k — Calculated for all Beckman Coulter high-speed rotors as a Erythrocytes — See RBC (red blood cells) measure of the rotor’s relative pelleting Ethidium bromide — A fluorescent intercalating...
Page 152 Glossary Granulocytes — Generic name for three leukocyte Noryl — Modified thermoplastic polyphenylene (white blood cell) types characterized by having oxide (PPO) used for floating spacers (part of the granules in their cytoplasm g-Max system) and some polycarbonate bottle caps (Noryl is a registered trademark of Hard spin —...
Page 153 -------- - is a mild solution that has been tested and found effective and safe for Beckman Coulter rotors where r is the radius in millimeters, ω is the and accessories angular velocity in radians per second (2π...
Page 154 Glossary WBC — White blood cells, or leukocytes, protect the body against infection and many diseases Wettable — Tube or bottle material that water or other aqueous solution will adhere to; the more wettable a tube or bottle material is, the more biological material, DNA, protein, cells, and so forth, will adhere to the walls Glossary-4...
Page 155 Subject to the conditions specified below and the warranty clause of the Beckman Coulter, Inc., terms and conditions of sale in effect at the time of sale, Beckman Coulter, Inc. agrees to correct either by repair, or, at its election, by replacement, any defects of material or workmanship which develop within seven (7) years after delivery of a J series rotor to the original buyer by Beckman Coulter, Inc.
Page 156 Beckman Coulter, Inc. J Series Rotor Warranty Warranty-2 PN JR-IM-10AG...
Page 158 Rotors and Tubes for Tabletop Preparative Chemical Resistances for Beckman Coulter • • Ultracentrifuges Centrifugation Products (IN-175) Rotors and Tubes for J2, J6, Avanti J Series Beckman Coulter High Performance, High • • Centrifuges Speed, High Capacity Rotors, Tubes &...

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Beckman Coulter Avanti J Series Instructions For Use Manual

Revision History

Safety Notice

Rotors

Tubes, Bottles, and Accessories

Tubes, Bottles, and Accessories

Using Fixed-Angle Rotors

Using Swinging-Bucket Rotors

And Rack-Type Rotors,6-1

Using Vertical-Tube and Rack-Type Rotors

Care and Maintenance

Products,A-1

Chemical Resistances for Beckman Coulter Centrifugation Products

Temperature Compensation

Gradient Materials

Blood Component Separation

References

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