Beckman Coulter Avanti J-26XP Instructions For Use Manual
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Beckman Coulter Avanti J-26XP Instructions For Use Manual

High-performance centrifuge
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J326XP-IM-5AB
January 2012
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821
Instruction For Use
Avanti J-26XP
High-Performance Centrifuge

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  • Page 1 Instruction For Use Avanti J-26XP High-Performance Centrifuge J326XP-IM-5AB January 2012 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821...
  • Page 2 Avanti J-26XP High-Performance Centrifuge J326XP-IM-5AB (January 2012) Copyright © 2012 Beckman Coulter, Inc. All rights reserved. No part of this document may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without prior written permission from Beckman Coulter, Inc.

  • Page 3: Safety Notice

    This instrument is designed to be installed by a Beckman Coulter Field Service representative. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument. Also, if the instrument needs to be moved, a Beckman Coulter Field Service representative must reinstall and relevel the instrument in its new location.

  • Page 4: Electrical Safety

    Mechanical Safety For safe operation of the equipment, observe the following: • Use only the Beckman Coulter rotors and accessories designed for use in this instrument. • Do not exceed the maximum rated speed of the rotor in use. • NEVER attempt to slow or stop a rotor by hand.
  • Page 5 Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. • Dispose of all waste solutions according to appropriate environmental health and safety guidelines. It is your responsibility to decontaminate the instrument and accessories before requesting service by Beckman Coulter Field Service. J326XP-IM-5AB...
  • Page 6 Safety Notice Chemical and Biological Safety J326XP-IM-5AB...

  • Page 7: Table Of Contents

    Contents Safety Notice, iii Alerts for Danger, Warning, Caution, and Note, iii Safety During Installation and/or Maintenance, iii Electrical Safety, iv Safety Against Risk of Fire, iv Mechanical Safety, iv Chemical and Biological Safety, iv Introduction, xiii Certification, xiii Scope of This Manual, xiii Conventions, xiv CFC-Free...

  • Page 8: Introduction

    Name Rating Plate, 2-3 Controls and Indicators, 2-3 Power Switch, 2-3 Control Panel, 2-4 CHAPTER 3: Introduction, 3-1 Summary of Avanti J-26XP Run Procedures, 3-2 Section and Loading, 3-3 Entering Run Parameters, 3-4 Selecting a Rotor, 3-4 Setting Run Speed, 3-4...
  • Page 9 Contents Storage and Transport, 5-5 Storage, 5-5 Returning a Centrifuge, 5-5 Supply List, 5-6 Replacement Parts and Supplies, 5-6 APPENDIX A: Introduction, A-1 Avanti® J Series Centrifuge Warranty, Warranty-1...
  • Page 10 Illustrations Single-Phase Electrical Requirements, 1-9 Three-Phase “Y” Electrical Requirements, 1-9 Correct and Incorrect Three-Phase Power Configurations, 1-10 Rear View and Dimensions, 1-12 Checking the Rotor for Drive Pins, 1-13 The Power Switch, 2-4 The Control Panel, 2-4 Acceleration and Deceleration Settings Graph, 3-8 Elutriation Controls, 3-9...
  • Page 11 Tables Tables Nominal Supply Voltage Ratings for the Avanti J-26XP, 1-8 Required Wire Connections, 1-10 Acceleration Settings, 3-7 Deceleration Settings, 3-8 Diagnostic Message Chart, 4-2...
  • Page 12 Tables...

  • Page 13: Certification

    Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual is the sole responsibility of the user.

  • Page 14: Conventions

    CFC-Free Centrifugation To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the Avanti J-26XP centrifuge. Radio Interference This instrument has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of FCC Rules.

  • Page 15: Recycling Label

    For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.
  • Page 16 Introduction Recycling Label J326XP-IM-5AB...

  • Page 17: Specifications

    CHAPTER 1 Specifications and Preinstallation Requirements Specifications Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Control Features Specifications Description Speed • Setting range: — 100 to 26 000 rpm (in 100-rpm increments below 10 000 rpm, 500-rpm increments above 10 000 rpm), or equivalent RCF (in 100 ...

  • Page 18: Operational Features

    Specifications and Preinstallation Requirements Specifications Specifications Description Acceleration maximum or slow Deceleration maximum, slow, or off a. During transient conditions, such as acceleration and deceleration, rotor temperature may be outside this range. Refer to applicable rotor manuals for specific rotor operating range information. b.

  • Page 19: Available Rotors

    Specifications and Preinstallation Requirements Available Rotors Specification Description Electrical Requirements • 200/208/240-V, single-phase instrument — 180–264 VAC, 30 A, 50/60 Hz • 230-V, single-phase instrument — 180–264 VAC, 30 A, 50 Hz • 220/380-V plus neutral, three-phase instrument — 313–457 VAC plus neutral, 16 A, 50 Hz Electrical Supply Class I Maximum Heat...
  • Page 20 Specifications and Preinstallation Requirements Available Rotors Rotor Rotor Manual Rotor Profile Description Code ( g) Max Capacity Number 24 15 mL JA-25.15 Ti 25.15 25 000 J-TB-057 Fixed Angle, 25° (24 place) = 106 mm 74 200 (outer row) = 86 mm 60,200 (inner row) 18 ...
  • Page 21 Specifications and Preinstallation Requirements Available Rotors Rotor Rotor Manual Rotor Profile Description Code ( g) Max Capacity Number 6  250 mL JA-14 14 000 30 100 J-TB-004 Fixed Angle, 25° (6 place) = 137 mm 14  50 mL F14BCI-14x50cy F50C 14 000...
  • Page 22 Specifications and Preinstallation Requirements Available Rotors Rotor Rotor Manual Rotor Profile Description Code ( g) Max Capacity Number 6  50 mL JS-13.1 13.1 13 000 26 500 J-TB-036 Swinging Bucket (6 place) = 140 mm 6  38.5 mL JS-24.38 24.38 10 000...

  • Page 23: Preinstallation Requirements

    Temperature performance for the JA-30.50 Ti rotor in the Avanti J-26XP is as follows: 15°C minimum at 26 000 rpm (at 30°C ambient); 4°C minimum at 21 500 rpm (30°C ambient). c. Maximum speed for the JA-30.50 Ti rotor is 26 000 rpm in the Avanti J-26XP centrifuge, and 30 000 rpm in the Avanti J-30I centrifuge.

  • Page 24: Electrical Requirements

    Terminate the open end of the harmonized cord with a certified single- or three-phase connector suitable for the power supplied in the country of intended use (see Table 1.1). Install only one centrifuge per circuit. Table 1.1 Nominal Supply Voltage Ratings for the Avanti J-26XP Instrument Nominal Instrument Part Number...

  • Page 25: Single-Phase Electrical Requirements

    Specifications and Preinstallation Requirements Preinstallation Requirements Single and Three-Phase Power Connections Figure 1.1 shows the power connection for single-phase centrifuges, including earth-ground and two power leads with 30-ampere circuit breakers. Figure 1.2 shows the power connection for three- phase centrifuges, including earth-ground and three power leads with minimum 16-ampere circuit breakers and common neutral connection.

  • Page 26: Correct And Incorrect Three-Phase Power Configurations

    Specifications and Preinstallation Requirements Preinstallation Requirements Table 1.2 Required Wire Connections Symbol Wire Insulation Color Terminal Harmonized North American Green/Yellow Earth ground Light Blue Neutral Brown Live or Line Black (three-phase Live or Line (qty 2) — connections only) Additional Requirements for Three-Phase Power Connections For three-phase power service to the centrifuge, use the “...

  • Page 27: Space And Location Requirements

    Refer to this document for additional installation instructions. NOTE Beckman Coulter representatives are not equipped to drill holes in your floor. The holes must be drilled before your scheduled installation. J326XP-IM-5AB...

  • Page 28: Rear View And Dimensions

    Specifications and Preinstallation Requirements Preinstallation Requirements Figure 1.4 Rear View and Dimensions 58.5 45.5 1.8 m 6.0 ft. 16.0 6.25 1. Air Exit 4. Depth 2. Power Cable 5. Air Intake 3. Air Diverter Bio-Safety Level 3 Installation For laboratories with epoxy aggregate (resinous) floors, such as BSL-3 labs, a non-invasive installation kit (PN 393316) is available.

  • Page 29: Using J2 Series Rotors In The Avanti J

    1.5). These drive pins engage with the centrifuge spindle hub to ensure that the rotor does not slip during acceleration. Some Beckman Coulter rotors, including the JA-10, the JS-7.5, the JA-14, and the JS-13, have been manufactured without drive pins because pins were not needed when these rotors were used in J2 series centrifuges.

  • Page 30: Using The Ja-18 Rotor

    If you do not see two metal pins in the hole, do not use the rotor in the Avanti J. Call your local Beckman Coulter office for information on returning the rotor to the factory for upgrading.
  • Page 31 Beckman Coulter fixed angle rotors. If rotor misidentification occurs when your JCF-Z rotor is used, first perform three runs from 0 to 5000 rpm and back to 0 rpm. If rotor misidentification recurs, replace the bearings.
  • Page 32 Specifications and Preinstallation Requirements Using J2 Series Rotors in the Avanti J 1-16 J326XP-IM-5AB...

  • Page 33: Chapter 2: Centrifuge Function And Safety Features

    Centrifuge Function and Safety Features Centrifuge Function The Avanti J-26XP is a refrigerated centrifuge that generates centrifugal forces required for a wide variety of applications. Together with the Beckman Coulter rotors designed for use in this centrifuge, applications include: •...

  • Page 34: Safety Features

    Safety Features Avanti J-26XP centrifuges have been designed and tested to operate safety indoors at altitudes up to 2 000 m (6 562 ft). Safety features include the following.

  • Page 35: Friction Reduction System (Frs)

    The name rating plate is affixed to the rear of the centrifuge. Check that the line voltage agrees with the voltage listed on this name rating plate before connecting the centrifuge. Always mention the serial number and the model number shown when corresponding with Beckman Coulter regarding your centrifuge.

  • Page 36: Control Panel

    Description Controls and Indicators Figure 2.1 The Power Switch Control Panel The control panel (Figure 2.2) is mounted at an angle on the centrifuge top rear for easy visibility and access. It contains a digital display for actual and setup values, knobs and touch keys for parameter entry, and additional touch keys for system control.
  • Page 37 ROTOR Knob Used to select the rotor in use. As the knob is turned, the name of each Beckman Coulter rotor that can be run in the centrifuge appears in succession in ROTOR the ROTOR ID display, and the LED by each rotor type abbreviation (JCF: continuous flow rotors;...
  • Page 38 Description Controls and Indicators TIME Selection Two time modes are available. The HR:MIN mode is used for runs of specified lengths. The HOLD mode is used for continuous runs of unspecified lengths. The HOLD key is used to toggle between the two modes. In HR:MIN mode: •...
  • Page 39 Description Controls and Indicators System Keys START Pressed to begin a run. When START is pressed, the display immediately shows the actual centrifuge values. The green START light flashes during acceleration until set speed is reached, and then remains on continuously until deceleration begins. STOP Pressed to end a run;...
  • Page 40 Description Controls and Indicators J326XP-IM-5AB...
  • Page 41 CHAPTER 3 Operation Introduction This section contains detailed centrifuge operating procedures. A summary is provided on page 3-2. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps. WARNING Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive.

  • Page 42: Summary Of Avanti J-26Xp Run Procedures

    Operation Summary of Avanti J-26XP Run Procedures Summary of Avanti J-26XP Run Procedures For runs at temperatures other than ambient, precool or prewarm the rotor to the required temperature before the run. Press the switch to on ( ). Open the centrifuge door.

  • Page 43: Section And Loading

    Operation Section and Loading Section and Loading For fast temperature equilibration, precool or prewarm the rotor to the required temperature before the run. Turn the power switch on ( • Power is applied to the system, and the display illuminates. Step on the foot pedal to open the door.

  • Page 44: Entering Run Parameters

    Operation Entering Run Parameters Entering Run Parameters Selecting a Rotor Turn the knob until the rotor in use appears in the display. ROTOR • Small green LEDs by each rotor type abbreviation light one at a time, and the rotors of that type appear consecutively in the display.

  • Page 45: Setting Run Time

    Operation Entering Run Parameters Turn the knob until the required RPM appears in the display. SPEED SPEED • When the run is started, the centrifuge operates at the set speed. • The corresponding RCF is automatically calculated. a. Press to display the RCF value. RPM/RCF Setting RCF Check to see if the red...
  • Page 46 Operation Entering Run Parameters Timed Run Turn the knob until the required run time appears in the display. TIME TIME • If you enter more than 59 minutes in the minutes field, the system automatically converts the entry to hours and minutes after is pressed.

  • Page 47: Setting Run Temperature

    Operation Entering Run Parameters Setting Run Temperature Select a run temperature between –10 and +40°C, in 1°C increments. NOTE For runs at temperatures other than ambient, always refrigerate or warm the rotor to the required run temperature before the run. For low-temperature runs, precool the system by running a precooled rotor at 2000 rpm at the required temperature for at least 30 minutes.

  • Page 48: Acceleration And Deceleration Settings Graph

    Operation Entering Run Parameters Table 3.2 Deceleration Settings Maximum Deceleration Slow Deceleration Full brake is used from set Reduced torque is used from set speed to No brake is used. Rotor speed to near 0 rpm, to 500 rpm, causing deceleration to 500 rpm to coasts to 0 rpm.

  • Page 49: Setting Up An Elutriation Run

    Operation Entering Run Parameters Press the key to toggle between the acceleration settings. ACCEL SLOW • The selected setting lights. SLOW ACCEL Setting the Deceleration Rate Press the key to toggle between the , and deceleration settings. DECEL SLOW • The selected setting lights. SLOW DECEL Setting Up an Elutriation Run...
  • Page 50 Operation Entering Run Parameters Install the rotor. Set up sample and buffer reservoirs and tubing lines as described in the rotor manual. Route the tubing lines through the elutriator port holes at the left side of the door seal. b. Close the centrifuge door. Turn the knob to the position.

  • Page 51: Starting A Run

    Operation Entering Run Parameters When the rotor speed has stabilized, look through the port in the centrifuge door and turn the knob until the elutriation chamber in the rotor is synchronized with the strobe. DELAY • (The chamber will appear to be motionless.) •...

  • Page 52: Changing Parameters During A Run

    Operation Changing Parameters During a Run Changing Parameters During a Run While a run is in progress, the speed, time, temperature, and acceleration or deceleration setting can be changed without stopping the run. The rotor name parameter cannot be changed during a run: Changing Time Settings To increase or decrease the remaining run time, turn the knob to the new run time.

  • Page 53: Unloading The Centrifuge

    Operation Unloading the Centrifuge Press to initiate deceleration. STOP • The light on the key flashes until the rotor comes to a stop. STOP After the rotor stops spinning, step on the foot pedal to open the door. NOTE During runs at very cold temperatures, nominally –10°C or below, ice may form around the door opening, causing the door to stick shut.
  • Page 54 Operation Unloading the Centrifuge 3-14 J326XP-IM-5AB...

  • Page 55: Chapter 4: Introduction

    CHAPTER 5, Care and Maintenance. For any problems not covered here, contact Beckman Coulter Field Service (1-800-742-2345 in the United States for assistance. NOTE It is your responsibility to decontaminate the instrument, as well as any rotors and/or accessories, before requesting service by Beckman Coulter Field Service.

  • Page 56: Diagnostic Message Chart

    If you are unable to correct the problem, call Beckman Coulter Field Service (in the United States, call 1-800-742-2345; outside of the U.S., call your local Beckman Coulter office). To help the Field...
  • Page 57 SPEED Speed control system is Rotor spinning above Call Beckman Coulter Field malfunctioning set speed; run shuts Service. down with maximum brake DOOR Door is not latched...
  • Page 58 Keep the door closed as much as possible. a. In the United States, call 1-800-742-2345. Outside the U.S., contact your local Beckman Coulter office. J326XP-IM-5AB...

  • Page 59: Accessing The Rotor In Case Of Power Failure

    Troubleshooting Accessing the Rotor in Case of Power Failure Accessing the Rotor in Case of Power Failure WARNING Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and/or mechanical injury. therefore, turn the power off ( ) and disconnect the instrument from the main power source, and refer such maintenance to qualified service personnel.

  • Page 60: Manual Door Release

    Troubleshooting Accessing the Rotor in Case of Power Failure Turn the power switch to off ( ) and unplug the power cord from the main source. Insert a 4-mm or -in. Allen wrench straight through one of the holes (either one) and turn the wrench to the left (counterclockwise) about one-half turn.
  • Page 61 Troubleshooting Accessing the Rotor in Case of Power Failure Pull the interlock lever out and to the left (at about a 45-degree angle), and while holding it out, step on the foot pedal. • Depending on the level of vacuum in the chamber at the time of the power failure, the door may or may not open.

  • Page 62: Venting The Chamber Vacuum

    Troubleshooting Accessing the Rotor in Case of Power Failure Figure 4.3 Venting the Chamber Vacuum 1. Step 1 5. Grasp red hose and pull up Do not disconnect the tubing lines tied to the red hose. 2. Step 2 6. Red hose removed from fitting 3.

  • Page 63: Jcf-Z Rotor Identification

    The magnets ensure correct identification. Call your local Beckman Coulter Field Service representative for more information. JCF-Z rotors manufactured after March, 1997, have factory-installed magnets. * In the United States, call 1-800-742-2345. Outside the U.S., contact your local Beckman Coulter office. J326XP-IM-5AB...
  • Page 64 Troubleshooting JCF-Z Rotor Identification On the first few uses of a new JCF-Z rotor, or in an older JCF-Z rotor with newly replaced bearings, run the rotor from 0 to 5000 rpm and back to 0 rpm three times. • This procedure will distribute lubricant around the bearings in the rotating seal assembly, reducing drag.

  • Page 65: Chapter 5: Introduction

    Inspect the centrifuge chamber for accumulations of sample, dust, or glass particles from broken sample tubes. a. Clean as required (see below). Cleaning * In the United States, call 1-800-742-2345. Outside the U.S., contact your local Beckman Coulter office. J326XP-IM-5AB...

  • Page 66: Cleaning

    Care and Maintenance Maintenance Check the air filter on the back panel for obstructions. a. Keep vents clear and clean. Wipe condensation out of the chamber between runs with a sponge or clean cloth to prevent chamber icing. If chamber icing occurs, defrost the system and wipe moisture out of the chamber before use. a.

  • Page 67: Decontamination

    IN-175 for chemical resistances of centrifuge and accessory materials. While Beckman Coulter has tested ethanol (70%) and found that it does not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 68: Replacing The Air Filter

    Care and Maintenance Maintenance Replacing the Air Filter Check the air filter regularly and replace it about once a year, or more often if it looks dirty. • The air filter is not fastened to the centrifuge, so no tools are required for removal or installation.

  • Page 69: Circuit Breaker And Fuses

    Storage and Transport Storage To ensure that the centrifuge does not get damaged, contact Beckman Coulter Field Service for specific instructions and/or assistance in preparing the equipment for transport or long-term storage. Temperature and humidity conditions for storage should meet the environmental...

  • Page 70: Supply List

    Care and Maintenance Supply List Supply List Refer to the High Performance, High Speed, High Capacity Rotors, Tubes & Accessories catalog (BR-8102, available at www.beckmancoulter.com) and the applicable rotor manual for materials and supplies needed for rotors. For your convenience, a partial list of centrifuge supplies is given below. Replacement Parts and Supplies Description Part Number...

  • Page 71: Appendix A: Introduction

    Temperature Calibration Procedure Introduction The Avanti J-26XP centrifuge specification for temperature control is 2°C of the set temperature. That means that your sample will stay within 2°C of set temperature at all times, after rotor and system equilibration, described below. (During transient conditions, such as acceleration and deceleration, the rotor temperature may be outside this range.) The following procedure is...
  • Page 72 Temperature Calibration Procedure Introduction If the measured and set temperatures are different, note how many degrees different they are and adjust the set temperature up or down that number of degrees. For example: If the required sample And the measured buffer/ temperature is water temperature is Set the temperature to...

  • Page 73: Avanti® J Series Centrifuge

    Any product claimed to be defective must, if requested by Beckman Coulter, be returned to the factory, transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless the product is found to be defective, in which case Beckman Coulter will pay all transportation charges.
  • Page 74 Beckman Coulter, Inc. Avanti® J Series Centrifuge Warranty Warranty-2 J326XP-IM-5AB...
  • Page 75 Symbol Simbolo Symbol Title Titel Titre Titulo Titolo Symbole Símbolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung)
  • Page 76 © 2012 Beckman Coulter, Inc. All Rights Reserved...

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