Tubes And Bottles - Beckman Coulter Avanti J Series Instructions For Use Manual

Using Fixed-Angle Rotors
Description
Rotor Knob
Daisy
Knob
CAUTION
Always loosen the rotor knob before loosening the daisy knob to avoid jamming
the knobs.
O-Ring
Sealing Surface
Fluid
Containment
Annulus
NOTE
Although rotor components and accessories made by other manufacturers may fit in the
Beckman Coulter rotor you are using, their safety in the rotor cannot be ascertained by Beckman Coulter.
Use of other manufacturers' components or accessories in the Beckman Coulter rotor may void the rotor
warranty, and should be prohibited by your laboratory safety officer. Only the components and
accessories listed in the applicable rotor manual should be used.

Tubes and Bottles

Fixed-angle rotors can accommodate a variety of tube types, listed in the rotor manual. Refer to
CHAPTER 3 for tube filling and sealing requirements. Observe the maximum rotor speeds and fill
volumes listed in the rotor manual.
NOTE
JLA-8.1000 and JLA-9.1000 rotors run only the specially designed bottles with polypropylene AutoVent
plug and polyphenylsulfone (PPSU) or polyphenylene sulfide (PPS) cap/closures. Refer to the applicable
rotor manual for instructions on use of these bottles and accessories.
4-6
Some rotors have dual-locking lid mechanisms consisting of a daisy knob
that secures the lid to the rotor, and a round rotor knob that attaches the
rotor to the centrifuge drive spindle hub. (Daisy refers to the knob shape.
The grooves between each "petal" let your fingers grip the knob firmly and
provide leverage for turning.) The dual-locking capability provides added
biosafety by allowing the rotor to be loaded into and removed from the
centrifuge with the lid in place. The rotor may be placed under a safety
hood before the lid is attached or removed.
A feature of many Beckman Coulter fixed-angle rotors is a patented
fluid-containment annulus, located below the O-ring sealing
surface. If tubes are overfilled or if leakage occurs during
centrifugation, the annulus holds enough volume that all of the
liquid is kept inside the rotor—even if all tubes leak at the same
time. This feature virtually eliminates the escape of liquid into the
centrifuge chamber.
PN JR-IM-10AG