Loading Buckets; Using Modular Disk Adapters - Beckman Coulter Avanti J Series Instructions For Use Manual

Loading Buckets

Buckets can be loaded before or after being installed on the rotor yoke. For best results, fill the
appropriate labware first and then load the labware into the buckets. This is especially important
when using blood bags—you can trip the imbalance detector in the centrifuge by pushing blood
bags into cups within buckets that are installed in the rotor. You can also bend the centrifuge drive
spindle.

Using Modular Disk Adapters

Assemble modular disk adapters as follows.
1
Select the appropriate adapter base and attach a bail to it.
2
Place the base and bail in an empty bucket or on the lab bench (not in the rotor).
3
Position one of the disks so that its grooves are aligned with the bail.
a. Push the disk down until the bail snaps into the grooves.
4
Add more disks until the height of the assembly is nearly as tall as the tubes you will be using.
(If the height of the disks is very tall, you may have to push the bail into the grooves of the top
disks by hand.)
a. Remove or add disks to the bail to accommodate shorter or longer tubes.
b. If the tubes fit too snugly in the adapter's rubber base, apply a light film of dusting power,
such as talcum powder, to prevent the tubes from sticking.
Place each tube in an adapter so that its weight is balanced by a tube in a diametrically opposite
position across the pivotal axis in the same adapter. Adapters placed in opposing buckets should
also be filled the same way (see
tube rests over the bucket's pivotal axis.
NOTE
Be sure to run a tube of the same approximate weight in the same configuration in the opposite
bucket.
PN JR-IM-10AG
Disk
Base
Figure 5.2). If you must run only one tube in an adapter, be sure this
Using Swinging-Bucket Rotors
Rotor Preparation and Loading
Bails
5
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