Vertical-Tube Rotors; Rack-Type Rotors; Vertical-Tube Rotor; Rack-Type Rotor - Beckman Coulter Avanti J Series Instructions For Use Manual

Using Vertical-Tube and Rack-Type Rotors
Description

Vertical-Tube Rotors

Figure 6.1 Vertical-Tube Rotor
r
min
r
av
r
max
Axis of Rotation

Rack-Type Rotors

The rack-type rotor (see
are loaded into removable trays, which are then loaded into carriers at a resting angle. During
centrifugation, the carriers swing out to a completely horizontal position to provide uniform
pelleting of samples. The nearly vertical position of the racks during centrifugation permits
processing of up to 320 mL in one run.
Figure 6.2 Rack-Type Rotor
r
min
r
av
r
max
Axis of Rotation
6-2
Vertical-tube rotors (see Figure
therefore, bands separate across the diameter of the tube rather than down the
length of the tube (see Figure
banding subcellular particles. These rotors have plugs that are screwed into the rotor
cavities over sealed Quick-Seal tubes. The plugs (with spacers, when required)
restrain the tubes in the cavities and provide support against the hydrostatic force
generated by centrifugation. Refer to
sealing Quick-Seal tubes for use in vertical-tube rotors.
Figure 6.2) holds a wide range of gamma-counter tubes in tube racks. Racks
6.1) hold tubes parallel to the axis of rotation;
1.3). Vertical-tube rotors are useful for separating and
CHAPTER 3 for information about filling and
PN JR-IM-10AG