Using Blood Bag Cups - Beckman Coulter Avanti J Series Instructions For Use Manual

Using Swinging-Bucket Rotors
Rotor Preparation and Loading

Using Blood Bag Cups

Do not pour liquid directly into blood bag cups. Fit blood bags into cups before loading the cups into
the rotor buckets. Load the blood bag cups as follows:
1
Load the cups so that the blood bags and tubing fit as far down as possible.
NOTE
Make sure the bags stay as vertical as possible, with no folds at the top or corners. If folds are
present, blood cells could remain in the folds and then mix with the plasma when the bag is removed.
2
Sandwich the tubing between the blood bag and any transfer packs (see
3
Make sure the loaded blood bag cups opposite each other on the rotor yoke are approximately
the same weight (within limits listed in the applicable rotor manual).
In some rotors, balancing pads can be used if necessary to maintain weight balance.
4
Place loaded cups into rotor buckets.
a. If only two filled cups are run, place them in opposing buckets.
The remaining buckets should contain similar "blank" loads to prevent imbalance (either
empty modular disk adapters or water-filled blood bags in cups).
CAUTION
If bucket covers or rotor lids are not used, make sure the superstructure of the
blood bag protruding from the cup does not inhibit the bucket from reaching its
horizontal position. If it does, remove the cup from the rotor and reposition the
blood bag so that it seats further into the cup. Allowing the blood bags to contact
the rotor yoke during centrifugation can cause the bucket to come off the pivot
pins and can seriously damage both the rotor and the centrifuge.
5-12
Figure 5.3).
PN JR-IM-10AG