Beckman Coulter J2-MC Manuals

Manuals and User Guides for Beckman Coulter J2-MC. We have 1 Beckman Coulter J2-MC manual available for free PDF download: Instructions For Use Manual

Beckman Coulter J2-MC Instructions For Use Manual

Beckman Coulter J2-MC Instructions For Use Manual (158 pages)

Rotors and Tubes

Brand: Beckman Coulter | Category: Laboratory Equipment | Size: 5.45 MB

Table of Contents

Revision History

3
Safety Notice

5
Rotors

19
Tubes, Bottles, and Accessories

41
- Introduction
  41
- Labware Selection Criteria
  41
- Labware Types
  43
  - Polypropylene Tubes
    43
    
    Open-Top Polypropylene Tubes
    43
    
    Quick-Seal Polypropylene Tubes
    44
  - Polycarbonate Tubes
    44
  - Polyethylene Tubes
    44
  - Ultra-Clear Tubes
    44
  - Stainless Steel Tubes
    45
  - Microfuge Tubes
    45
  - Bottles
    45
  - Multiwell Titer Plates
    45
    
    96-Well Microtiter Plates
    45
    
    Deep-Well Titer Plates (and Caps)
    46
    
    Square-Well Titer Plates
    46
- Temperature Limits
  46
- Spacers and Floating Spacers
  47
- Adapters
  47
  - Bottle Adapters
    47
  - Multitube Adapters
    47
    
    Solid Multitube Adapters
    48
    
    Modular Disk Adapters
    48
- Bottle and Tube Caps
  49
- Aerosolve Canisters
  49
- Blood Bag Cups
  50
- Rotor Labware Assemblies
  50
Tubes, Bottles, and Accessories

51
- Introduction
  51
- Gradient Preparation
  51
- General Filling and Sealing or Capping Requirements
  52
  - General Filling and Sealing Requirements for Tubes and
    53
  - Working with Physiological Fluids
    54
- Filling Open-Top Tubes
  55
  - Open-Top Polypropylene Tubes
    55
    
    Swinging-Bucket Rotors
    55
    
    Fixed-Angle Rotors
    55
  - Other Open-Top Tubes
    55
    
    Polycarbonate
    55
    
    Ultra-Clear
    55
    
    Polypropylene
    56
    
    Polyethylene
    56
    
    Stainless Steel
    56
- Capping Tubes
  56
- Filling and Capping Tubes
  57
  - Three-Piece Assemblies
    57
  - JLA-8.1000 and JLA-9.1000 Bottle Cap/Closure
    57
- Filling and Loading Cups in the
  58
- Rotor
  58
- Filling and Sealing Quick-Seal Tubes
  59
  - The Cordless Quick-Seal Tube Topper
    60
  - Capping Multiwell Titer Plates
    64
    
    Cap Strips
    64
    
    Aluminum Foil Lids
    64
  - Using Adapters
    64
    
    Using Solid Multitube Adapters
    64
    
    Using Modular Disk Multitube Adapters
    65
- Using Aerosolve Canisters
  66
- Using Blood Bag Cups
  69
- Sample Recovery
  70
  - Capped Tubes
    71
  - Cups
    71
  - Quick-Seal Tubes
    72
- Making Ultra-Clear Tubes Wettable
  73
Using Fixed-Angle Rotors

75
- Introduction
  75
- Description
  75
- Rotor Preparation and Loading
  81
  - Prerun Safety Check
    81
  - Rotor Preparation
    81
- Operation
  82
  - Installing the Rotor
    83
  - Removal and Sample Recovery
    84
Using Swinging-Bucket Rotors

87
- Introduction
  87
- Description
  87
- Rotor Preparation and Loading
  91
- Operation
  103
- Sample Recovery
  103
  - Removing JS-24 Series Rotors
    103
And Rack-Type Rotors,6-1

105
Using Vertical-Tube and Rack-Type Rotors

105
- Introduction
  105
- Description
  105
  - General Specifications for Beckman Coulter J Series Vertical-Tube and Rack-Type Rotors
    105
  - Vertical-Tube Rotors
    106
  - Rack-Type Rotors
    106
  - Vertical-Tube Rotor
    106
  - Rack-Type Rotor
    106
- Using a Vertical-Tube Rotor
  107
  - Tubes and Bottles
    107
  - Rotor Preparation and Loading
    107
    
    Prerun Safety Checks
    107
    
    Rotor Preparation
    107
  - Operation
    108
  - Installing the Rotor
    109
  - Removal and Sample Recovery
    110
- Using a Rack-Type Rotor
  111
  - Trays and Tubes
    111
  - Rotor Preparation and Loading
    111
    
    Prerun Safety Checks
    111
    
    Rotor Preparation
    111
  - Operation
    112
  - Installing the Rotor
    113
  - Removal and Sample Recovery
    114
Care and Maintenance

115
- Introduction
  115
- Rotor Care
  115
  - Cleaning
    116
  - Decontamination
    117
  - Sterilization and Disinfection
    118
  - Inspection
    118
  - Lubrication
    119
    
    O-Rings
    119
    
    Pivot Pins and Buckets
    119
  - Field Rotor Inspection Program
    120
- Tube, Bottle, and Accessory Care
  120
  - Cleaning
    120
  - Decontamination
    121
  - Sterilization and Disinfection
    121
  - Tube and Bottle Sterilization and Disinfection
    122
  - Inspection
    123
  - Tube and Bottle Storage
    123
- Returning a Rotor or Accessory to the Factory
  124
- Diagnostic Hints
  124
  - Troubleshooting Chart
    125
Products,A-1

129
Chemical Resistances for Beckman Coulter Centrifugation Products

129
- List of Chemical Resistances
  129
- Temperature Compensation Tables
  131
- Introduction
  131
Temperature Compensation

131
Gradient Materials

137
- Introduction
  137
Blood Component Separation

143
- Introduction
  143
- Blood Bank Collection Overview
  143
- Components and Typical Usage
  144
  - Single-Donor Fresh Plasma
    144
  - Single-Donor Plasma
    144
  - Packed Red Blood Cells
    144
  - Leukocyte-Depleted Red Blood Cells
    144
- Shelf Life
  145
- Freezing
  145
- Separation of Blood Components by Centrifugation
  145
  - Blood Component Storage
    145
  - Blood Component Separation
    146
  - Blood Bank Methods
    147
- Tips for Optimum Centrifugation Runs
  148
References

149
- List of References
  149

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