Beckman Coulter Avanti J-26S XP Series Instructions For Use Manual

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Instructions for use manual (72 pages)

Instructions for use manual (158 pages)

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Page 1 Instructions For Use JS-5.3 Swinging-Bucket Rotor For Use in Beckman Coulter Avanti J-26S XP Series, Avanti J-26 XP Series, Avanti JXN-26, and Avanti J-E Centrifuges PN J-TB-089AG March 2014 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
Page 2 Beckman Coulter, Inc. Avanti, Beckman Coulter, and the stylized logo are trademarks of Beckman Coulter, Inc. and are registered in the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.
Page 3: Safety Notice
This rotor was developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter centrifuge/rotor system. Their safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter’s approval.
Page 4 Although rotor components and accessories made by other manufacturers may fit in the JS-5.3 rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the JS-5.3 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer.
Page 5: Table Of Contents
Speeds, 14 Care and Maintenance, 16 Maintenance, 16 Cleaning, 18 Decontamination, 19 Sterilization and Disinfection, 20 Storage, 20 Returning a Rotor, 21 Supply List, 22 Replacement Rotor Parts, 22 Supplies, 22 Beckman Coulter, Inc. J Series Swinging-Bucket Rotor Warranty Related Documents...
Page 6 Illustrations Illustrations Rotor Drive Pin Location and Orientation, 3 Symmetrical and Balanced Loading, 6 Correct and Incorrect Alignment of Microplates in the Bucket, 8 Tube Height Limits for Certain Tube Positions in Adapter 392071, 8 Tube Height Limits for Certain Tube Positions in Adapter 392072, 8 Orientation of Capped Tubes in Corner Positions of Adapter 392074, 9...
Page 7 Tables Tables Microplates Used in the JS-5.3 Rotor, 12 Tube and Bottle Adapters for the JS-5.3 Rotor, 13 Relative Centrifugal Fields for the JS-5.3 Rotor, 15...
Page 8 Tables viii...
Page 9: Js-5.3 Swinging-Bucket Rotor
JS-5.3 Swinging-Bucket Rotor Specifications Maximum speed ......... . 5300 RPM Critical speed range .
Page 10: Description
Beckman Coulter JS-5.3 rotors are manufactured in a facility that maintains certifications to both ISO 9001:2008 and ISO 13485:2003. They are for use with the specified Beckman Coulter centrifuges. The JS-5.3 is a four-place swinging-bucket rotor used in Beckman Coulter Avanti JXN-26, J-26S XP series, Avanti J-26 XP series, Avanti J-E, and discontinued J-20 XP series centrifuges.
Page 11: Prerun Safety Checks
The drive pins are positioned parallel to the rotor yoke arm on which is engraved (see Figure 1). Note the drive pin BECKMAN COULTER orientation as you follow the steps below. Figure 1 Rotor Drive Pin Location and Orientation Rotor Pins Beckman Coulter...
Page 12 JS-5.3 Swinging-Bucket Rotor Preparation and Use Carefully lower the rotor straight down onto the centrifuge drive hub. CAUTION Carefully lower the rotor yoke straight down onto the drive spindle hub to avoid bending the drive spindle/hub assembly. Do not drop the rotor onto the hub. Slowly rotate the rotor yoke to make sure it is properly seated.
Page 13: Loading The Buckets
JS-5.3 Swinging-Bucket Rotor Preparation and Use Loading the Buckets For runs at other than room temperature, refrigerate or warm the rotor and precool the centrifuge beforehand for fast equilibration. WARNING Handle body fluids with care because they can transmit disease. No known test offers complete assurance that such fluids are free of micro-organisms.
Page 14: Symmetrical And Balanced Loading
JS-5.3 Swinging-Bucket Rotor Preparation and Use Figure 2 Symmetrical and Balanced Loading Examples of Symmetrically Loaded Adapters Examples of Symmetrically Loaded Trays (Load Opposite Trays the Same Way) Example of Nonsymmetrically Loaded Tray * All four positions must have a bucket attached for every run. PN J-TB-089AG...
Page 15 JS-5.3 Swinging-Bucket Rotor Preparation and Use Using Buckets You can load buckets before or after they are installed on the rotor yoke. In either case, we recommend filling the appropriate labware first and then loading the labware into the buckets to avoid tripping the imbalance detector.
Page 16: Correct And Incorrect Alignment Of Microplates In The Bucket
JS-5.3 Swinging-Bucket Rotor Preparation and Use Using Covers NOTE Covers can be used only with blue buckets (368706). They cannot be used with previously manufactured black buckets (368415). Always visually check to make sure that the labware fits under the cover before your attempt to secure the latches.
Page 17: Orientation Of Capped Tubes In Corner Positions Of Adapter 392074
JS-5.3 Swinging-Bucket Rotor Preparation and Use — In adapter 392074, capped tubes placed in the four corner positions must be oriented so that the cap hinges and tabs do not extend towards the corners of the adapter (see Figure Figure 6 Orientation of Capped Tubes in Corner Positions of Adapter 392074 Leave Clear Install the covers as follows.
Page 18: Operation
JS-5.3 Swinging-Bucket Rotor Preparation and Use Operation Temperatures may vary slightly between centrifuges. If sample temperature is crucial, test temperature settings on your instrument using water samples. For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. Refer to the instrument instruction manual for centrifuge operation.
Page 19: Labware
Labware Temperature Limits • Beckman Coulter plastic adapters and carriers have been centrifuge tested for use at temperatures between 2 and 40°C. For centrifugation at other temperatures, pretest labware under anticipated run conditions. • If plastic containers are frozen before use, make sure that they are thawed to at least 2°C prior to centrifugation.
Page 20 JS-5.3 Swinging-Bucket Rotor Preparation and Use Table 1 Microplates Used in the JS-5.3 Rotor Accessory Part Part Description Volume Number Description Number 267002 cap strip, nonsterile (pkg/12) multiwell polystyrene plate, 96-well, 267005 cap strip, sterile 300 mL/well 609844 nonsterile (pkg/12) 538619 aluminum foil lid (pkg/100)
Page 21 JS-5.3 Swinging-Bucket Rotor Preparation and Use Table 2 Tube and Bottle Adapters for the JS-5.3 Rotor Maximum RCF at Max Part Tubes/ Speed Adapter Number Tube/Bottle Bottles per Adapter Color (Pkg/4) Size Adapter (RCF) 6145 ´ g beige 13 mm dia. 392071 6145 ´...
Page 22: Run Speeds
JS-5.3 Swinging-Bucket Rotor Preparation and Use Table 2 Tube and Bottle Adapters for the JS-5.3 Rotor (Continued) Maximum RCF at Max Part Tubes/ Speed Adapter Number Tube/Bottle Bottles per Adapter Color (Pkg/4) Size Adapter (RCF) 6870 ´ g blue 500 mL conical 392078 356966 6670 ´...
Page 23 JS-5.3 Swinging-Bucket Rotor Preparation and Use Table 3 Relative Centrifugal Fields for the JS-5.3 Rotor Deep-well Plates 500-mL Conical Bottles Relative Centrifugal Field ´ g Relative Centrifugal Field ´ g Rotor Rotor At r At r At r At r At r At r Speed...
Page 24: Care And Maintenance
If any of these signs are evident, do not run the rotor. Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center. Before using the rotor, inspect the rotor drive pins to ensure that they are not damaged.
Page 25: Bucket Lubrication Area
JS-5.3 Swinging-Bucket Rotor Care and Maintenance Before each use, inspect the covers to make sure they are in good condition. Do not use a cover that is cracked or damaged. Inspect each O-ring to make sure that is smooth and free of nicks, tears, and abrasions. Make sure that it has not stretched beyond its original shape and size.
Page 26: Cleaning
Store the rotor in a dry environment (not in the centrifuge). Refer to Chemical Resistances (publication IN-175) for the chemical compatibilities of rotor and accessory materials. Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center. Cleaning Wash rotor components immediately if salts or other corrosive materials are used or if spillage has occurred.
Page 27: Decontamination
If aluminum rotor components become contaminated with radioactive material, decontaminate them using a solution that will not damage the anodized surfaces. Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum: RadCon Surface Spray or †...
Page 28: Sterilization And Disinfection
Ethanol is a flammability hazard. Do not use it in or near operating centrifuges. using any other sterilization methods. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.
Page 29: Returning A Rotor
Returning a Rotor Before returning a rotor or accessory for any reason, prior permission must be obtained from Beckman Coulter, Inc. A return form may be obtained from your local Beckman Coulter sales office. The form should contain the following information: •...
Page 30: Supply List
NOTE Publications referenced in this manual can be obtained at www.beckmancoulter.com, by calling Beckman Coulter at 1-800-742-2345 in the United States, or by contacting your local Beckman Coulter office. See the Beckman Coulter High Performance, High Speed, High Capacity Rotors Tubes & Accessories catalog (BR-8102, available at www.beckmancoulter.com) for detailed information on ordering...
Page 31 JS-5.3 Swinging-Bucket Rotor Supply List Description Part Number Rotor Cleaning Kit 339558 Solution 555 (1 qt) 339555 Rotor cleaning brush 339379 Paint On Graphite Lubricant (1/2 oz) 977212 Silicone vacuum grease (1 oz) 335148 PN J-TB-089AG 1-23...
Page 32 JS-5.3 Swinging-Bucket Rotor Supply List 1-24 PN J-TB-089AG...
Page 33: Beckman Coulter, Inc
A defective rotor will be replaced by Beckman Coulter at its then current list price less a credit based upon the age of the rotor (years since date of purchase). The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter’s Indianapolis, Indiana facility or delivered...
Page 34 Beckman Coulter, Inc. J Series Swinging-Bucket Rotor Warranty Warranty-2 PN J-TB-089AG...
Page 36 PN IN-175 Available in electronic pdf or CD-ROM by request. High Performance, High Speed, High Capacaity Rotors, Tubes, & Accessories PN BR-1802 Available in hard copy by request. Available at www.beckmancoulter.com www.beckmancoulter.com © 2014 Beckman Coulter, Inc. All Rights Reserved...

This manual is also suitable for:

Avanti j-26 xp series Avanti jxn-26 Avanti j-e

Beckman Coulter Avanti J-26S XP Series Instructions For Use Manual

Safety Notice

JS-5.3 Swinging-Bucket Rotor

Beckman Coulter, Inc

J Series Swinging-Bucket Rotor Warranty