Performing A Fractionation - Agilent Technologies 3100 OFFGEL Fractionator User Manual

Performing a Fractionation

Fractionations using the Agilent 3100 OFFGEL Fractionator can be performed
in the OFFGEL mode or in the standard IPG IEF mode (in-gel mode).
The table lists the default methods delivered with the system and ready to use
with a cooling platform temperature of 20°C
Table 6
Method name
OG24PR01
OG24PE01
OG12PR01
OG12PE01
IG_24_00
IG_13_00
DIAGNOSE
OFFGEL User Manual
Default methods
recommended use
OFFGEL default method for proteins
for use with 3100 OFFGEL High Res Kit, pH 3-10, 24 well frames
OR
for use with 3100 OFFGEL High Res Kit, pH 4-7, 24 well frame
OFFGEL default method for peptides
for use with 3100 OFFGEL High Res Kit, pH 3-10, 24 well frames
OR
for use with 3100 OFFGEL High Res Kit, pH 4-7, 24 well frame
OFFGEL default method for proteins
for use with 3100 OFFGEL Low Res Kit, pH 3-10, 12 well frames
OR
for use with 3100 OFFGEL Low Res Kit, pH 4-7, 12 well frame
OFFGEL default method for peptides
for use with 3100 OFFGEL Low Res Kit, pH 3-10, 12 well frames
OR
for use with 3100 OFFGEL Low Res Kit, pH 4-7, 12 well frame
IPG-IEF in-gel default method for protein and peptides
for use with 24 cm IPG-strips pH 3-10 OR pH 4-7.
IPG-IEF in-gel default method for protein and peptides
for use with 13 cm IPG-strips pH 3-10 OR pH 4-7.
use for hardware diagnosis as described in
Electrodes and Electrode Connectors"
Operation

Performing a Fractionation

"Checking High Voltage,
on page 97.
5
65