Agilent Technologies 3100 OFFGEL Fractionator User Manual page 106

7 Troubleshooting
Troubleshooting the Application
Figure 45
The run stopped when the volt hour limit of 20 kVh was reached. Both volt
signal and power signal are increasing throughout the run, since the sample is
depleted of ions and thus the resistance is increasing. After fractionation, the
protein test samples should be focused in fraction 3 (see
106
electrical
current
charge
Example 1- typical results for the Protein test sample using the 3100 OFFGEL
Low Res Kit, pH 3-10.
power
Volt
kVhours
Figure 46).
OFFGEL User Manual