Agilent Technologies 3100 OFFGEL Fractionator User Manual page 98

7 Troubleshooting
Troubleshooting the Instrument
Figure 43
No currents should flow in the empty tray lanes (<1.0 µA). If this is the case
proceed with
contaminated electrodes, electrode connectors or trays. Clean and carefully
dry the electrodes, trays and the electrode connectors as described in
"Cleaning the Agilent 3100 OFFGEL Fractionator"
test again at
Typical values for currents are between 20 µA and 50 µA and one lane has to
be at maximum current of 50 µA (representing the current limit) if the voltage
is just at 800 V or below 800 V. In case the voltage is constantly at 800 V
(representing the voltage limit) a current lower than 50 µA might be present in
all lanes. Differences in current from lane to lane are natural and might be due
to variation in the resistance of the electrode surface. The voltage has to be
greater than 500 V.
The absolute value for the voltage is dependent on the conductivity of the deionized water
N O T E
used and is not a criterion for the proper function of the instrument. The more salt ions the
water contains, the lower is the voltage value.
10 Open the top cover and pipette 4 ml deionized water (18 MOhm/m) into
11 Close the top cover.
98
Typical current pattern on tray I when every second lane is filled with water.
step 10. Otherwise stop here because you may have
step 6.
lanes B, D, F and H on each tray.
on page 70 and start this
OFFGEL User Manual