Troubleshooting The Application - Agilent Technologies 3100 OFFGEL Fractionator User Manual

7 Troubleshooting

Troubleshooting the Application

Troubleshooting the Application
Impaired focusing results can have many different root causes. In addition to
hardware errors, the impaired focusing results can be sample and/or handling
related. Therefore, always make sure that you set up your focusing experiment
exactly as described in the kit manual. The sample concentration should be
between 50 µg/ml and 5 mg/ml and the salt content of your sample buffer
should be at most 10 mM. Run only samples similar in protein content and
buffer composition on the same tray, otherwise it is highly recommended that
you use separate trays.
With every focusing, a results file containing all electrical parameters during a
run are stored to the compact flash card and can be exported. The traces also
provide useful information for troubleshooting (see
Results"
"Looking at Run Parameters"
The following table gives an overview of symptoms you might experience when
performing OFFGEL experiments, their possible causes and actions required
for remedy.
102
on page 58), and some examples are discussed in this chapter (see
on page 105)
"How to Display Exported
OFFGEL User Manual