Agilent Technologies 3100 OFFGEL Fractionator User Manual
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Agilent Technologies 3100 OFFGEL Fractionator User Manual

Agilent Technologies 3100 OFFGEL Fractionator User Manual

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Summary of Contents for Agilent Technologies 3100 OFFGEL Fractionator

  • Page 1 Agilent 3100 OFFGEL Fractionator User Manual Agilent Technologies...
  • Page 2 Notices Warranty © Agilent Technologies, Inc. 2010 receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June No part of this manual may be reproduced in The material contained in this docu- 1987). U.S. Government users will receive any form or by any means (including elec- ment is provided “as is,”...
  • Page 3 Site requirements and specifications This chapter describes information about site requirements that have to be met so that the Agilent 3100 OFFGEL Fractionator performs within specifications. OFFGEL User Manual...
  • Page 4 PC, Browser and LAN Requirements This chapter describes information about requirements for your PC, browser and LAN that have to be met for the control of the Agilent 3100 OFFGEL Fractionator. Installation This chapter describes how to install the Agilent 3100 OFFGEL Fractionator.
  • Page 5 Related Documents The Agilent 3100 OFFGEL Fractionator can be used with different consumable kits which each are supplied with a Kit Manual: • 5969-1582 Kit Manual OFFGEL User Manual...
  • Page 6 OFFGEL User Manual...

  • Page 7: Table Of Contents

    Contents Contents Product overview Intended Use Fractionation Principles of the Agilent 3100 OFFGEL Fractionator Features Instrument and Accessories Site requirements and specifications Site Requirements for the Agilent 3100 OFFGEL Fractionator Power Considerations Bench Space Environment The Waste Electrical and Electronic Equipment (WEEE) Directive...
  • Page 8 How to Display Exported Results Instrument Configuration Additional Information Performing a Fractionation Preparing a Tray for the OFFGEL Mode Preparing a Tray for the Standard IPG IEF Mode Starting a Run Maintenance Cleaning the Agilent 3100 OFFGEL Fractionator Cleaning the Electrodes OFFGEL User Manual...
  • Page 9 Installation of the Firmware Update Tool How to un-install the Firmware Update Tool Installation of the Firmware on your PC Connect your PC to the Agilent 3100 OFFGEL Fractionator Setup of the Firmware Update Tool Configure the Firmware Update Tool...
  • Page 10 Contents Checking Fixed and Movable Electrodes Troubleshooting the LAN Connection Troubleshooting the Application Looking at Run Parameters Example 1. Typical Results of the Protein Test Sample Example 2 . Current Drop During Fractionation Example 3. Reduced Electrical Charge in One Lane Safety Safety Information Power Cords...
  • Page 11 Contents Netzkabel Betrieb Sicherheitssymbole Chemische und biologische Sicherheit Sécurité Consignes de sécurité Cordons secteur Utilisation Symboles de sécurité Sécurité chimique et biologique Sicurezza Informazioni per la sicurezza Cavo di alimentazione Funzionamento Simboli di sicurezza Sicurezza chimica e biologica Seguridad Información de seguridad Cables de alimentación Operación Símbolos de seguridad...
  • Page 12 Contents OFFGEL User Manual...

  • Page 13: Product Overview

    Intended Use Features Instrument and Accessories This chapter gives an overview of the Agilent 3100 OFFGEL Fractionator. It describes the intended use and the fractionation principles of the instrument, highlights its features and outlines the different instrument components. Agilent Technologies...

  • Page 14: Intended Use

    Product overview Intended Use Intended Use The Agilent 3100 OFFGEL Fractionator system fractionates proteins or peptides based on their isoelectric points (pI) in liquid phase (OFFGEL mode). In addition, the system performs standard in-gel isoelectric focusing (IEF) using immobilized pH gradient gel (IPG) strips (in-gel mode). The system consists of an instrument that can be controlled via LAN by a User Interface based on a typical web browser.
  • Page 15 Product overview Intended Use Figure 1 Fractionation principle of the Agilent 3100 OFFGEL Fractionator. OFFGEL User Manual...

  • Page 16: Features

    Product overview Features Features • pI-based OFFGEL fractionation with in-solution recovery for easy transfer to downstream methods such as LC/MS. • Reproducible pI-based OFFGEL fractionation down to 0.1 pH resolution depending on the consumable kit used. • Preconfigured methods for OFFGEL and traditional in-gel IEF protein and peptide fractionation.

  • Page 17: Instrument And Accessories

    Product overview Instrument and Accessories Instrument and Accessories Figure 2 gives an overview of the complete Agilent 3100 OFFGEL Fractionator system: Top Cover Trays Cooling Platform Status LED Serial number On/Off Figure 2 Instrument overview OFFGEL User Manual...
  • Page 18 Product overview Instrument and Accessories The instrument has two individual high voltage power supplies which provide voltages to the two trays. On the left side of the cooling platform is the anode connector; across the cooling platform are two cathode connectors (see Figure Anode Connector Cathode Connectors...
  • Page 19 Product overview Instrument and Accessories Movable Electrode [Cathode] Frames Fixed Electrode [Anode] Tray Fixed Electrode [Anode] Movable Electrode [Cathode] Tray Cover Seals Figure 4 Electrodes, trays and frames The frames are included in the consumable kits. The available consumable kits are listed in Table Table 1...
  • Page 20 Product overview Instrument and Accessories OFFGEL User Manual...
  • Page 21 The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Sound Emission Instruction for Use Physical Specifications of the Agilent 3100 OFFGEL Fractionator This chapter describes information about site requirements that have to be met so that the Agilent 3100 OFFGEL Fractionator performs within specifications. Agilent Technologies...

  • Page 22: Site Requirements And Specifications

    50–60 Hz (see also on page 25 for more details). Consequently, there is no voltage selector in the rear of the instrument. Shock hazard or damage to your Agilent 3100 OFFGEL Fractionator may result if the C A U T I O N Agilent 3100 OFFGEL Fractionator is connected to a line voltage higher than specified.

  • Page 23: The Waste Electrical And Electronic Equipment (Weee) Directive (2002/96/Ec)

    Site requirements and specifications Site Requirements for the Agilent 3100 OFFGEL Fractionator Put the Agilent 3100 OFFGEL Fractionator only on a flat and horizontal work top. N O T E Inclinations of more than 0.5% will cause the instrument to malfunction.

  • Page 24: Instruction For Use

    • According to ISO 7779:1988/EN 27779/1991 (Type Test). Instruction for Use The Agilent 3100 OFFGEL Fractionator is designed and tested as general purpose electrical equipment for laboratory use. It is designed and tested for research use only and not for use in diagnostic procedures.

  • Page 25: Physical Specifications Of The Agilent 3100 Offgel Fractionator

    Site requirements and specifications Site Requirements for the Agilent 3100 OFFGEL Fractionator Physical Specifications of the Agilent 3100 OFFGEL Fractionator Table 2 Physical Specifications Type Specification Comment Weight 13 kg (29 lbs) Dimensions 157 x 355 x 427 mm Height × width × depth Line voltage 100–240 VAC...
  • Page 26 Site requirements and specifications Site Requirements for the Agilent 3100 OFFGEL Fractionator OFFGEL User Manual...
  • Page 27 Agilent 3100 OFFGEL Fractionator User Manual PC, Browser and LAN Requirements Browser Interface Tested Configurations This chapter describes information about requirements for your PC, browser and LAN that have to be met for the control of the Agilent 3100 OFFGEL Fractionator.

  • Page 28: Pc, Browser And Lan Requirements

    PC, Browser and LAN Requirements Browser Interface The Agilent 3100 OFFGEL Fractionator can be controlled from an internet browser interface. The communication of browser application and the instrument is based on the TCP/IP network protocol as it is used for display of webpages from the world wide web.

  • Page 29: Lan

    • a connection by a Cross-Over LAN cable Another successfully tested configuration consisted of: • a 3100 OFFGEL Fractionator with wired LAN connection to a local Network • Apple iPod Touch 8G (FW Revision 3.1.2, including a Safari Browser) with wireless LAN access to the local Network.
  • Page 30 PC, Browser and LAN Requirements PC, Browser and LAN Requirements OFFGEL User Manual...

  • Page 31: Installation

    Unpacking the Agilent 3100 OFFGEL Fractionator Setting up the Control PC Setting up the Agilent 3100 OFFGEL Fractionator This chapter describes how to install the Agilent 3100 OFFGEL Fractionator and set up a LAN connection to a PC. Agilent Technologies...

  • Page 32: Unpacking The Agilent 3100 Offgel Fractionator

    Make sure to retain the administrative rights until the system setup is complete. 2 Open the box in which the Agilent 3100 OFFGEL Fractionator was shipped. On the top you will find a box containing the accessories and a Declaration of Conformity.
  • Page 33 Installation Unpacking the Agilent 3100 OFFGEL Fractionator Figure 6 Instrument inner shipment box. 4 Remove the foam packing and the inner box and place the instrument in a suitable place. 5 Check that you have the complete set of accessories available using...

  • Page 34: Setting Up The Agilent 3100 Offgel Fractionator

    • Install the Compact Flash Card • Insert the USB memory stick • Connect the power cord The Agilent 3100 OFFGEL Fractionator has the following connectors at the rear: Configuration RS232...
  • Page 35 Switches N° 7 and 8 must be up 5 Switch on the instrument. Figure 8 Configuration switches of the Agilent 3100 OFFGEL Fractionator The instrument LED is usually yellow, which indicates that the cooling platform is cooling down to the specified temperature (typically 20°C). Once the temperature has been reached, the LED is switched off.

  • Page 36: Setting Up The Control Pc

    Assignment of an IP Address to the Control Computer The Control PC and the the 3100 OFFGEL fractionator need to be enabled for LAN communication by assigning the PC a suitable IP address first. This IP address is associated with a certain subnet address. The 3100 OFFGEL fractionator has a similar default IP address from the same subnet.

  • Page 37: Local Network Connection (Optional)

    N O T E (G3100-90111) shipped with the instrument. Do connect the PC only to the 3100 OFFGEL fractionator. Connecting the PC with this N O T E manually set IP address to your local area network will most likely create network issues.
  • Page 38 Installation Setting up the Control PC web-based User Interface. Please see the description of the web-based User Interface in Chapter “The User Interface” starting on page 40 and find addition information on the Instrument Configuration on page After you have set the custom IP address, subnet mask and gateway address, do the following steps: 1 Power the instrument off.
  • Page 39 Additional Information Performing a Fractionation This chapter details how to operate the Agilent 3100 OFFGEL Fractionator. It familiarizes the user with all control functions from the internet browser interface. It describes the necessary steps to perform a fractionation and to change the customizable IP address of the instrument.

  • Page 40: Operation

    Operation The User Interface The User Interface The Agilent 3100 OFFGEL Fractionator can be controlled from an internet browser interface. The communication of browser application and the instrument is based on the display of webpage content retrieved from the instrument. Please see Chapter 4 for establishing the LAN connection.
  • Page 41 2 OFFGEL interface tabs 3 Instrument clock 4 Platform temperature 5 Status indicator 6 USB indicator 7 Application Lock 8 Tray method 9 Method edit 10 Fractionation start Figure 9 Overview on Agilent 3100 OFFGEL Fractionator interface. OFFGEL User Manual...

  • Page 42: General Functions

    Operation General Functions General Functions A few elements of the screen shown in Figure 9 on page 41 are common to many tabs. Besides the Status indicator and the USB indicator this are the Instrument Clock and the Platform-Temperature. You can adjust these parameters by clicking onto this area.

  • Page 43: Status Tab

    Operation Status tab Status tab The Status tab shows the current state of both trays. Figure 11 shows Tray 1 in a run. The Status tab is also the main application screen which enables you to select and load methods, to start and stop them. The Status tab allows processing and modification of run parameters interactively of a method that is currently running.
  • Page 44 Operation Status tab fractionation. Not ready indication on for example tray temperature will lead to a waiting step. An open lid will cause the not ready information and application of the voltage is done only if the lid is finally closed, see also Table 8 on page 92.
  • Page 45 Operation Status tab The instrument is accessible from any computer in the local network. To protect it from unintended access an Instrument lock can be established. Click onto the lock icon and select a password. You might want to leave information for other users or a password hint for yourself in a free editable field.

  • Page 46: Working With Methods

    Operation Working with Methods Working with Methods The Method tab (see Figure 14) is used to view, modify and store methods. It also shows all method parameters and a graphical view of the timetable settings. In opposite to the edit options from the status tab which reflects an online editing mode, the Method tab allows offline editing and storing.
  • Page 47 Operation Working with Methods In a pull down menu for Method Selection you can select methods that are present on the internal drive of the instrument or on the USB stick inserted at the rear of the instrument. These methods can either be default methods which were supplied with the instrument or user modified methods.
  • Page 48 Operation Working with Methods Printing of methods is typically possible and is based on the browser features. Select the print button of the browser or select the print dialog under the browser file menu. Selected elements of the OFFGEL method, such as the values for focussing and hold phase, the graphical display of the timetable and the time table will be printed.

  • Page 49: Displaying Data Graphically

    Operation Displaying Data Graphically Displaying Data Graphically The plot tab (Figure 15) allows viewing up to 4 signals which can be Volt, Volt Hours, Voltage Limit, Current Limit and Current per lane. The signals are available from the Signal Selection Menu. The scaling source of the Y-Axis is selectable by clicking onto the data series in the Graph area or reselecting it from the menu.

  • Page 50: Zoom In Plots

    Operation Displaying Data Graphically Zoom in Plots Using a PC with a mouse connected, the mouse wheel can be used for zooming. Both axes scales can be changed independent from each other. The mouse wheel input will change Y-Scale as long as the mouse pointer is located nearby the Y-Axis inside the graph area.

  • Page 51: Switching The Y-Scale In Plots

    Operation Displaying Data Graphically Switching the Y-Scale in Plots The source for the Y-Axis scaling can be changed from one to another signal by clicking onto the colored line of the signal in the graph area. The color of the y-Axis will change to reflect the color of the signal it is related to.

  • Page 52: Instrument Logbook

    Operation Instrument Logbook Instrument Logbook The Logbook shows events occurred on the instrument. The list consists of all events since the last instrument start. It can be printed by selecting the print button or the print dialog of the browser. Figure 16 Instrument Logbook OFFGEL User Manual...
  • Page 53 Operation Instrument Logbook The log book entries consist of date and time for an event on the instrument. Each event is a ssigned a color-coded class: • Green – change in status • Blue – info event • Red – error An internal identification code serves as detailed information for the Agilent Service.

  • Page 54: Exporting Results

    In the Results Calendar you can select the month and day when results were created. Clicking onto a date populates the files available for this selection. You can download the Agilent 3100 OFFGEL Fractionator plotter for data analysis by Microsoft Excel at any date containing data files. Under Data...
  • Page 55 Operation Exporting Results analysis is a link for Macro Download of the two versions for Microsoft Office 2003 or 2007 available. Next to this you find the links for a Result file download. Under operating system Windows 7 with Internet Explorer 8 a download of result file is done best by a right-click onto the respective download link and selection of the option "Save Target As...
  • Page 56 Operation Exporting Results In case the instrument detects an USB-memory stick which is incompatible due to the N O T E formatting it will try to format memory to FAT-16. Please be aware that any information present on the stick will be lost. OFFGEL User Manual...

  • Page 57: Reviewing Result Files

    Operation Reviewing Result Files Reviewing Result Files Result files are either transferred to your control PC by direct download using the link from the result tab and the browser function “Save Target as…” or they were transferred to the USB stick mounted to the 3100 OFFGEL fractionators. In order to review them it is reasonable to store them in a typical location of the Computer used for reviewing them such as under “MyDocuments”.

  • Page 58: How To Display Exported Results

    Excel add-on and facilitates the analysis of the run parameters. The tool is downloadable from the instrument in revisions for Microsoft Excel 2003 and 2007. The tool is also provided via the Agilent Technologies web page http://www.agilent.com (Life Sciences and Chemical Analysis Solutions - Technical Support - Downloads &...
  • Page 59 You can close the Agilent 3100 OFFGEL Fractionator user interface by clicking the N O T E close window button. You can reopen it by clicking the Agilent 3100 OFFGEL Fractionator toolbar button. 4 Click Open File, select an exported file with the browser and open the file.
  • Page 60 Operation Reviewing Result Files Figure 21 Agilent 3100 OFFGEL Fractionator user interface - result file opened. 6 Select all run parameters which should be plotted. 7 Click Make Graphic. The plot is displayed on a separate worksheet File name/Run at /...
  • Page 61 Operation Reviewing Result Files If a worksheet is manually deleted or the data file is reloaded, the corresponding graph is N O T E deleted. If a plot to a certain worksheet is repeatedly generated, the previous plot version is deleted.

  • Page 62: Instrument Configuration

    Operation Instrument Configuration Instrument Configuration The Configuration tab (Figure 23) allows to setup and to review LAN configuration of the instrument. The active IP address that is currently in use is highlighted by a blue corona. On this tab you can also trigger module identification.
  • Page 63 Operation Instrument Configuration The Agilent 3100 OFFGEL Fractionator has two LAN configurations stored. They can be used alternatively. The selection which of the stored LAN configurations is used upon the next instrument start is done by configuration dip-switches at the rear of the instrument. Any change in the LAN configuration is applied only after the instrument has been restarted.

  • Page 64: Additional Information

    The About page provides information on the revision of the currently active firmware of the connected instrument. It provides links to helpful Agilent web pages such as the Agilent 3100 OFFGEL Fractionator for latest news on the instrument or to a download location for the firmware update tool. Here you find copyright information of the Webinterface and the used open source Javascript libraries.

  • Page 65: Performing A Fractionation

    Performing a Fractionation Performing a Fractionation Fractionations using the Agilent 3100 OFFGEL Fractionator can be performed in the OFFGEL mode or in the standard IPG IEF mode (in-gel mode). The table lists the default methods delivered with the system and ready to use with a cooling platform temperature of 20°C...

  • Page 66: Preparing A Tray For The Offgel Mode

    Operation Performing a Fractionation Please note that latest OFFGEL methods do have a voltage limit of 4500 V. This voltage limit N O T E reduces the risk of arcing and damage to the tray in case of dried out electrode pads. Preparing a Tray for the OFFGEL Mode To prepare a tray for the OFFGEL mode, refer to the Kit Manual (5969-1582) for detailed instructions.
  • Page 67 Operation Performing a Fractionation Distribute the mineral oil evenly across the whole tray lane by pipetting droplets across the N O T E lane. Make sure that no mineral oil is spilled out of the tray. 6 Start the run as described in “Starting a Run”...

  • Page 68: Starting A Run

    Operation Starting a Run Starting a Run 1 To start the fractionation, make sure you have configured the cooling platform according to your needs. For details, refer to “General Functions” on page 42. 2 From the Status screen of the connected instrument select the Method in the drop down menu of the tray you will use.

  • Page 69: Maintenance

    In addition, it provides instructions on how to update the firmware. Only perform maintenance on the Agilent 3100 OFFGEL Fractionator as specified in this chapter. Other maintenance and repairs must be done by Agilent Technologies trained personnel.

  • Page 70: Cleaning The Agilent 3100 Offgel Fractionator

    Maintenance Cleaning the Agilent 3100 OFFGEL Fractionator Cleaning the Agilent 3100 OFFGEL Fractionator Materials used in the instrument that may come in contact with liquid reagents are resistant to chemicals typically used for isoelectric focusing (IEF). Always turn off the instrument and unplug the power cord before doing any cleaning WA R N I N G procedures.

  • Page 71: Cleaning The Trays

    Maintenance Cleaning the Agilent 3100 OFFGEL Fractionator 1 Rinse the complete electrodes with hand-warm tap water. Do not exceed a water temperature of 50°C. 2 Use a mild detergent such as dishwashing detergent or a 10% SDS solution and a brush to clean the electrodes.

  • Page 72: Cleaning The Cooling Platform

    Maintenance Cleaning the Agilent 3100 OFFGEL Fractionator Do not use heat such as a hair dryer or dish washer to clean and dry the trays since the C A U T I O N material may be deformed. Additional trays are also available as spare parts see Table 15 on page 156.
  • Page 73 Maintenance Cleaning the Agilent 3100 OFFGEL Fractionator 1 Loosen the 4 screws on top of the anode connector with a hex key. Figure 25 Disassembling the anode connector. 2 Carefully lift the anode connector from the front (Figure 26). Take care to not damage the cables at the rear of the anode connector.
  • Page 74 Maintenance Cleaning the Agilent 3100 OFFGEL Fractionator Anode Connector Cables Figure 26 Detached anode connector. 3 Wipe the anode connector with water using a lab tissue or paper towel. 4 Wipe the anode connector with isopropanol using a lab tissue or paper towel to remove the mineral oil.

  • Page 75: Cleaning The Top Cover And The Housing Of The Instrument

    Maintenance Cleaning the Agilent 3100 OFFGEL Fractionator Cleaning the Top Cover and the Housing of the Instrument The top cover and the housing can be cleaned with a damp cloth either using water or a mild detergent. Do not use alcohols or ketones for the top cover, since the material is not resistant to C A U T I O N these solvents.

  • Page 76: Firmware Update

    At regular intervals, Agilent Technologies releases new firmware revisions of its products to provide new features or bug fixes. These revisions and the Firmware Update Tools are provided via the Agilent Technologies web page http://www.agilent.com (Life Science and Chemical Analysis Solutions - Technical Support - Downloads &...

  • Page 77: Installation Of The Firmware Update Tool

    The Firmware Update Tool has an un-install routine. If required, remove the Firmware Update Tool via the “Uninstall Firmware Update Tool” function within the program folder, see Figure Installation of the Firmware on your PC 1 Download the Agilent 3100 OFFGEL Fractionator firmware from the Agilent web site OFFGEL User Manual...

  • Page 78: Connect Your Pc To The Agilent 3100 Offgel Fractionator

    Update Tool. Connect your PC to the Agilent 3100 OFFGEL Fractionator Your PC has to be connected by a Cross-Over LAN cable or an normal LAN cable to the Agilent 3100 OFFGEL Fractionator (Please see instructions for Installation in Chapter 4).

  • Page 79: Setup Of The Firmware Update Tool

    Maintenance Firmware Update Note that the upgrade process with LAN will take proximately 5 minutes. An update with N O T E RS 232 cable connection can take up to 2 hours. No fractionations can be performed during this time. Setup of the Firmware Update Tool 1 Start the Firmware Update Tool via the shortcut (see Figure...

  • Page 80: Configure The Firmware Update Tool

    Maintenance Firmware Update Options Configure directories - Add/Delete/Modify Instrument - Support G1323B (active/not active) Help Help - About (revision of program) Table 7 Functions of Buttons Button Description Connect to module/system Disconnect from module/system Select for update of all modules of a system Start the update for all selected modules Add instrument to the list Delete instrument from the list...
  • Page 81 Maintenance Firmware Update as well to the default location (C:\Program Files\Agilent\LC Firmware Update Tool). Figure 29 Select the button Configure Directories Figure 30 Configure Directories The Protocol file is a text file that gives the complete status of the firmware update process.
  • Page 82 Maintenance Firmware Update 3 Select the Add Instrument button. Figure 31 Select the Add Instrument button. 4 Enter a name for the system (e.g. System 1) 5 Enter the TCP/IP address information in the instrument address field, e.g. 168.192.254.11 for the default address of the instrument. •...
  • Page 83 Maintenance Firmware Update 6 Press OK. Figure 33 Add instrument screen 7 Double-click on All Instruments to expand the view. If you have a control module G3101A connected in parallel and want to update the Control N O T E Module, select Options and check Support Control Module.

  • Page 84: Doing The Firmware Update

    Figure 35 Modules of "3100 OFFGEL #1" are listed Doing the Firmware Update The following describes the general workflow for a firmware update of the 3100 OFFGEL fractionator instrument. 1 Select a module. Figure 36 Current Firmware Information screen 2 Above screen shows the current firmware information 3 Select the Revision History tab.
  • Page 85 Maintenance Firmware Update Figure 37 Revision History Information screen 4 Select the Update Information tab. In this window, you can select the firmware for the main and resident part in the module. If no firmware is listed when you click on the file selection button, correct the firmware N O T E folder locations, see Figure 30...
  • Page 86 Maintenance Firmware Update Figure 38 Update Information screen 6 Select the Start Update button. All selected modules are updated. The update status is shown at the bottom of the screen. In case you also update the resident Firmware this will be done first followed by the main firmware. When the main firmware is being updated, the Status LED of the instrument is red and N O T E blinking.

  • Page 87: The Log-File

    Maintenance Firmware Update If you cancel an update, the module remains in the current mode, resident or main, N O T E depending when the process was stopped. It cannot be used until the update process has been restarted. At the end of the update, a report is shown. The update status is either PASS (successful) or FAILED (not successful), see Figure 40.

  • Page 88: Firmware Change Via Usb Stick

    N O T E your findings to our technical support specialists (http://www.chem.agilent.com/scripts/cag_techsupport.asp) or contact your local Agilent Technologies representative. Attach your log-file and details of your system, revision and errors. Firmware Change via USB Stick A convenient alternative to the Firmware Update Tool is the instrument Firmware update via USB stick.

  • Page 89: Steps For Firmware Change Via Usb

    5 Power the 3100 OFFGEL Fractionator off. 6 Insert the prepared USB stick to the USB port of the instrument. 7 Power the Agilent 3100 OFFGEL Fractionator on, during the restart the instrument will check the USB stick folders for relevant files and perform an update in case the installed firmware has a lower revision.
  • Page 90 Main firmware is automatically downgraded when the USB stick is mounted on startup and the directory X:\OFFGEL\UPDATE does not exist or is empty and exactly one .dlb file for the Agilent 3100 OFFGEL Fractionator with a firmware version older than the current version is present in the directory X:\ OFFGEL\DOWNDATE.

  • Page 91: Troubleshooting

    Agilent 3100 OFFGEL Fractionator User Manual Troubleshooting Troubleshooting the Instrument Troubleshooting the Electrodes Troubleshooting the LAN Connection Troubleshooting the Application This chapter provides support for troubleshooting the instrument and the application. Agilent Technologies...

  • Page 92: Troubleshooting The Instrument

    Troubleshooting Troubleshooting the Instrument Troubleshooting the Instrument Status Indicators Status LED The instrument has a status LED on the front top right position (see Figure 2 on page 17). With the power switched on, the LED can have the following states: Table 8 LED status overview...

  • Page 93: Status Bars

    Troubleshooting Troubleshooting the Instrument Firmware download Tool and update to the latest firmware revision. The resident section of the firmware is identical in many Agilent devices and modules. Its properties include: • The complete communication capabilities (CAN, LAN and RS-232C) •...
  • Page 94 Troubleshooting Troubleshooting the Instrument Table 10 Error messages due to wrong operation (continued) Error message Solution Result Storage Empty. No result data to copy. Insert a compact flash card (for details refer to CF-Card is empty or not present Figure 7 on page 34) No USB drive.
  • Page 95 Call Agilent limits. Technologies. In case you wish to call Agilent Service for support on the 3100 OFFGEL fractionator provide N O T E a detailed error description, exported results from your latest sample runs, diagnosis buffers, content of the instruments log book and firmware revision.

  • Page 96: Hardware Diagnosis

    Troubleshooting Troubleshooting the Instrument Hardware Diagnosis With the following few steps, you can verify if your instrument is performing to its specifications. Note that a meaningful hardware diagnosis requires a fully functional communication to the instrument. Checking the LED and the cover sensor 1 Switch on or restart the instrument and watch the Status LED during startup.

  • Page 97: Checking High Voltage, Electrodes And Electrode Connectors

    If this is not the case, check once again that the tray and the electrodes are completely dry and clean. Inspect the cathode connector for contamination and clean it if necessary. Make sure that no sparking occurs. If you cannot solve this problem call Agilent Technologies for further help. Otherwise, proceed with step 5 Press Stop to stop both runs.
  • Page 98 10. Otherwise stop here because you may have contaminated electrodes, electrode connectors or trays. Clean and carefully dry the electrodes, trays and the electrode connectors as described in “Cleaning the Agilent 3100 OFFGEL Fractionator” on page 70 and start this test again at step Typical values for currents are between 20 µA and 50 µA and one lane has to...
  • Page 99 Typical current pattern on tray I when every lane is filled with water. 13 Press Stop on both trays to stop the runs. If you require further assistance, call Agilent Technologies and send the corresponding N O T E results file of this diagnose test to the customer call center together with a detailed description, exported results from your latest sample runs, diagnosis buffers, content of the instruments log book and firmware revision.

  • Page 100: Troubleshooting The Electrodes

    Troubleshooting Troubleshooting the Electrodes Troubleshooting the Electrodes Electrodes can be used multiple times. They are designed to be robust but they must still be regarded as consumable. They might suffer from permanent coating of the surface and from residues formed by arcing events during fractionations.

  • Page 101: Troubleshooting The Lan Connection

    Troubleshooting the LAN Connection Troubleshooting the LAN Connection If your web interface to the Agilent 3100 OFFGEL Fractionator does not work correctly check for disconnected LAN cables at the rear of the instrument. Make sure you are using the LAN connector and not the CAN connector.

  • Page 102: Troubleshooting The Application

    Troubleshooting Troubleshooting the Application Troubleshooting the Application Impaired focusing results can have many different root causes. In addition to hardware errors, the impaired focusing results can be sample and/or handling related. Therefore, always make sure that you set up your focusing experiment exactly as described in the kit manual.
  • Page 103 Troubleshooting Troubleshooting the Application Table 12 Troubleshooting the OFFGEL application Symptom Possible Cause Action No current or very low current 1 No electrical contact or • check if there is any damage (less than 20% of the current impaired contact. to the electrodes .
  • Page 104 Troubleshooting Troubleshooting the Application Table 12 Troubleshooting the OFFGEL application (continued) Symptom Possible Cause Action 3 the corresponding sample • Run only samples similar in has higher salt protein content and buffer concentration or higher composition on the same protein/peptide content tray.

  • Page 105: Looking At Run Parameters

    The results of all run parameters can be exported as a .csv file and thus can be visualized with Microsoft Excel. Agilent Technologies has designed the Agilent 3100 OFFGEL fractionator plotter which is a Excel add-on and facilitates the analysis of the run parameters (see “How to Display Exported Results”...
  • Page 106 Troubleshooting Troubleshooting the Application electrical power current Volt kVhours charge Figure 45 Example 1- typical results for the Protein test sample using the 3100 OFFGEL Low Res Kit, pH 3-10. The run stopped when the volt hour limit of 20 kVh was reached. Both volt signal and power signal are increasing throughout the run, since the sample is depleted of ions and thus the resistance is increasing.

  • Page 107: Example 2 . Current Drop During Fractionation

    Troubleshooting Troubleshooting the Application Figure 46 Beta-lactoglobulin Protein Test Sample in well 3 of a 12 cm pH 3-10 IPG strip. (Gel-like image from an Agilent 2100 bioanalyzer analysis using the Protein 80 kit of wells 1-9.) Example 2 . Current Drop During Fractionation Figure 47 shows an example where the current (see arrow) dropped close to zero after approximately 30 minutes of the run.

  • Page 108: Example 3. Reduced Electrical Charge In One Lane

    Troubleshooting Troubleshooting the Application Figure 47 Example 2- current drop during fractionation. The root cause of this focusing failure was that the IPG strip was dried out at one position so that the conductivity was lost. Example 3. Reduced Electrical Charge in One Lane Figure 48 shows an example of a fractionation of eight samples in parallel and just highlights the different electrical charges (in µAh) that were measured...
  • Page 109 Troubleshooting Troubleshooting the Application Figure 48 Example 3- one lane shows reduced electrical charge. The root cause of this failure was a conductivity problem in lane F. OFFGEL User Manual...
  • Page 110 Troubleshooting Troubleshooting the Application OFFGEL User Manual...

  • Page 111: Safety

    Agilent 3100 OFFGEL Fractionator User Manual Safety Safety Information This chapter contains safety information relevant to the Agilent 3100 OFFGEL Fractionator. Agilent Technologies...

  • Page 112: Safety Information

    Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of the Agilent 3100 OFFGEL Fractionator. All safety instructions should be read and understood before installation, operation and maintenance of the instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture and intended use of the instrument.

  • Page 113: Operation

    Never operate your instrument from a power outlet that has no ground connection. WA R N I N G Never use a power cord other than the Agilent Technologies power cord designed for your region. Never use cables other than those supplied by Agilent Technologies to ensure proper WA R N I N G functionality and compliance with safety or EMC regulations.

  • Page 114: Safety Symbols

    Safety Safety Information Safety Symbols Table 13 Safety Symbols used on Instruments and in Manuals Symbol Description The apparatus is marked with this symbol when the user should refer to the User Manual in order to prevent risk of harm to the operator and to protect the apparatus against damage.
  • Page 115 Because leaks, spills, or loss of sample may generate aerosols, observe proper safety precautions. Agilent 3100 OFFGEL Fractionator covers have not been designed as bioseals for aerosol or liquid containment. Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms.
  • Page 116 Safety Safety Information OFFGEL User Manual...

  • Page 117: 安全性

    Agilent 3100 OFFGEL フラクショネーター ユーザーマニュアル 安全性 安全性について この章では、 Agilent 3100 OFFGEL フラクショネーターに関する重要な安全 性情報を紹介します。 Agilent Technologies...

  • Page 118: 安全性について

    安全性 安全性について 安全性について Agilent 3100 OFFGEL フラクショネーターの操作、保守、修理を行う際には、 以下の安全性に関する一般的な注意事項を必ずお守りください。 装置の取り付け、操作、メンテナンスを行う前に、安全性に関する指示をお読 みになり、理解する必要があります。これらの諸注意、あるいは本書の他の箇 所に記載されている特定の警告に従わなかった場合には、装置の設計、製造、 および使用目的の安全基準に違反します。 当社は、顧客がこれらの条件や指示に従わなかった場合、それによって生じる いかなる損失に対しても、一切その責任を負いません。 警告は、人身事故や装置にダメージを与える可能性がある状況を警告しま 警 告 す。 示された条件を完全に理解し、その条件に適合するまで、この警告よ り先に進まないでください。 注意は、 Agilent 3100 OFFGEL フラクショネーター の機能や性能に障害を 注意 与え、データの紛失を引き起こす可能性がある状況を警告します。 示され た条件に完全に対応できるまで、この注意より先に進まないでください。 装置の安全性 注 本器は、Safety Class I の装置です ( 感電防止用アース端子を備えています )。 本器の製造およびテストは、国際的な安全基準に基づいて行われています。 OFFGEL ユーザー...

  • Page 119: 電源コード

    安全性 安全性について 電源コード Agilent 3100 OFFGEL フラクショネーターでは、オプションとして様々な電源 コードが提供されます。 電源コードの雌側はすべて同一の形状です。 装置の背 面に位置する電源入力ソケットへ電源コードを差し込みます。 電源コードの雄 側の形状は、特定の国や地域の壁面の差込口に適合する設計になっています。 アース接続のない電源の差込口へプラグをつないだまま装置を操作しない 警 告 ようにしてください。地域の基準に合うよう設計された Agilent Technologies 社の電源コード以外は使用しないでください。 安全基準や EMC 規則に準拠して正しく機能させるため、Agilent 警 告 Technologies 社が提供するケーブル以外は使用しないでください 操作 電気を供給する前に、取り付けの指示に従ってください。 装置を安全に操作するためです。 操作中は装置のカバーを外さないでください。 装置をオンにする前に、すべての感電防止用アース端子、延長コード、自動変圧器、および 装置に接続されたデバイスを、感電防止用アース・ソケットに接続する必要があります。感 電防止用アース線を断線させると、人体に危険の及ぶおそれがある感電事故を引き起こす可 能性があります。感電防止機能が損なわれていると思われる場合は、装置の使用を中止し、 手を触れないようにしてください。 電圧をかけた状態での装置のメンテナンスは避けてください。 電源を入れたまま、装置のコンポーネントを交換しないでください。 本装置を可燃性のガスや煙のある場所で使用しないでください。電気機器をこうした環境で 操作すると、安全性が損なわれます。 代替部品を取り付けたり、装置を許可無く改造しないでください。...

  • Page 120: 安全記号

    安全性 安全性について 安全記号 表 13 装置およびマニュアルで使用される安全記号 記号 説明 装置上にこのマークが表示されている箇所では、ユーザは 必ずユーザマニュアルを参照してください。理解していな いとオペレーターに危害があったり、装置が損壊したりす るおそれがあります。 化学および生物的な安全性 溶剤を扱うときには ( 特に、毒性または危険性のある溶剤を使用するとき 警 告 には )、溶剤メーカーが作成した材料の取り扱いおよび安全に関するデー タ・シートの説明に従い、適切な安全対策 ( ゴーグル、安全手袋、防護服 の着用など ) を講じてください。 OFFGEL ユーザー マニュアル...
  • Page 121 安全性 安全性について 本装置で病原性サンプル、毒性サンプル、または放射性サンプルの使用を 警 告 予定している場合は、必要とされるすべての安全に関する規制、ガイドラ イン、予防措置、慣例をユーザが責任を持って厳守する必要があります。 これには、遺伝子変異生物も含まれます。アプリケーションで要求される 遮蔽 ( 封じ込め ) のレベル、および液体が容器から漏れ出した場合にとる 適切な除染または殺菌手順については、所属する実験室の安全管理責任者 におたずねください。 使用前に、溶液の元の容器に印刷されているすべての注意事項を遵守して ください。 サンプルが漏れたり、こぼれたり、流出すると、エアロゾールが生成され る可能性があるため、適切な安全対策を講じてください。 Agilent 3100 OFFGEL フラクショネーターのカバーは、エアロゾールや液 体を遮蔽するためのバイオシールとして設計されていません。 体液は病気の感染源となるおそれがあるため、体液の取り扱いには注意し てください。これまでのところ、微生物が存在しないことを完全に保証す るテストはありません。B 型および C 型肝炎ウィルス、HIV (I-V) ウィル ス、異型マイコバクテリア、ある種の浸透性のかびなど、非常に有毒な微 生物に対しては、さらにエアロゾール対策を強化する必要があります。 汚染されたコンパートメントフレームなど、生物学的に危険な廃棄物を処 分するときには、常に、政府および各自治体の定める生物学的危険処理規 制に従ってください。 病気が広がらないように、感染性のサンプルはすべて、実験室の手順やメ ソッドに従って処理してください。 残った溶液や製品はすべて、該当する環境衛生および安全性のガイドライ ンに従って廃棄してください。...
  • Page 122 安全性 安全性について OFFGEL ユーザー マニュアル...
  • Page 123 Agilent 3100 OFFGEL 分馏器 用户手册 安全 安全说明 本章介绍了与 Agilent 3100 OFFGEL 分馏器相关的重要安全说明。 Agilent Technologies...

  • Page 124: 安全说明

    安全 安全说明 安全说明 在操作、维护和检修 Agilent 3100 OFFGEL 分馏器的过程中必须遵守以下常规安 全操作规程。 在对设备进行安装、操作和维修保养之前务必认真阅读和理解全部安全说明。不 遵从操作规程或无视手册中提出的特别警告将会违反仪器的设计、制造和使用安 全标准。 安捷伦科技对由于使用者违反操作规程所导致出现的问题不承担任何责任。 警告 （WARNING）为危险提示。提醒您遵守操作规范，如果操作不当可能会导致 警告 人身伤害或损坏设备。在完全理解并满足所示条件之前不要进行警告提示之后的 操作。 小心 （CAUTION）为危险提示。提醒您遵守操作规范，如果操作不当可能会 小心 削弱 Agilent 3100 OFFGEL 分馏器的功能或性能和导致数据丢失。在完全理 解并满足所示条件之前不要进行小心提示下面的操作。 设备安全 注意 本设备的安全级别为 I （配置有一个地线端口） ，根据国际安全标准制造和检 验。 电源线 Agilent 3100 OFFGEL 分馏器提供不同的电源线选件。所有电源线与仪器连接的 一头都是一样的，插在仪器后面的电源插槽里。电源线与电源连接的插头各不相 同，与不同国家和地区的墙上插座相匹配。...

  • Page 125: 安全标志

    安全 安全说明 电源插座没有接地时不得操作仪器。只能使用为所在地区专门设计的安捷伦科技 警告 公司的电源线。 为确保正常功能，并符合安全或 EMC 规范的要求，不得使用非安捷伦科技公司 警告 的电缆。 操作 接通电源之前，首先应阅读安装说明。另外，还要遵守以下规定。 为了安全地操作仪器，操作过程中不要取下仪器盖。在仪器开启之前，要检查全 部接地端、电缆、变压器和与仪器相连的设备是否都与地线插座相连。接地保护 中的任何一点故障，将有引起电击的可能，造成严重的人身伤害。如果有任何现 象表明接地保护环节上存在有不安全的可能性，请立即停止仪器运行并对已有的 操作进行安全检查。 请勿在通电情况下维护仪器。 带电状态下不要更换仪器的部件。 操作仪器时周围环境中不要有易燃 气体， 在有易燃气体的环境中操作任何电器 设备都会导致安全危害。 不要安装替代部件或对仪器做任何未经授权的修改。 安全标志 表 13 在仪器上和手册中使用的安全标志 标志 说明 有此种标志的设备在被使用时，操作者应当阅读有关 用户手册 的 以免对操作者和仪器造成损害。 OFFGEL 用户手册...

  • Page 126: 化学和生物安全

    安全 安全说明 化学和生物安全 使用溶剂时请遵循相应的安全操作规程 （如佩带防护眼镜，安全手套和穿防护 警告 衣） ，请按照试剂商提供的安全数据表和处理溶剂的有关说明进行操作，当所用 的试剂有毒有害时，这一点尤其重要。 如果要在该仪器上测试致病的、有毒的或是有放射性的样品，操作者有责任确保 警告 全部必要的安全规程、指南、措施。其中也包括合理处置有基因修饰的样品。如 果发生液体从容器中泄露的情况，务必根据实验室安全负责人的建议启动相应安 全级别的防泄漏系统以及合适的净化和灭菌程序。 在使用试剂之前要仔细阅读印在包装盒上的警示信息。 泄漏，溢撒或遗失的样品可能悬浮于空气中，要根据适当的安全提示采取相应的 措施。 Agilent 3100 OFFGEL 分馏器的盖子没有防止样品挥散和液体被污染的功能。 处理体液样品时务必小心，因为这些体液可能会传播疾病，目前还没有相应的检 测确保体液不含微生物致病菌。一些毒性很强的致病微生物，如肝炎病毒 （B 型 和 C 型） ，HIV 病毒 （I 型到 V 型） ，非典型分支杆菌，某些类型的真菌——更需 要防止体液气化扩散。 对于使用过的有生物毒性的废物，如污染了的分割框架应根据当地或国家关于有 毒生物样品处理规范条例加以处理。 对于有感染性的样品应根据实验室操作规范加以妥善处理，避免疾病的传播。 所有废弃液体和产物的处理应符合环境保护和健康安全的要求。 OFFGEL 用户手册...

  • Page 127: Sicherheit

    Agilent 3100 OFFGEL Fractionator Benutzerhandbuch Sicherheit Sicherheitshinweise Dieses Kapitel enthält wichtige Sicherheitshinweise zu dem Agilent 3100 OFFGEL Fractionator. Agilent Technologies...

  • Page 128: Sicherheitshinweise

    Sicherheitshinweise Die folgenden allgemeinen Sicherheitsmaßnahmen müssen in allen Phasen des Betriebs, der Wartung und der Reparatur des Agilent 3100 OFFGEL Fractionator. Die Bediener sollten vor der Installation, dem Betrieb und der Wartung des Geräts alle Sicherheitsanweisungen sorgfältig durchgelesen und verstanden haben.

  • Page 129: Netzkabel

    Landes. Betreiben Sie Ihr Gerät niemals an einem Netzanschluss ohne Erdung. Benutzen Sie WARNUNG niemals ein anderes Netzkabel als das von Agilent Technologies für Ihren Bereich deklarierte Netzkabel. Benutzen Sie keine anderen als die von Agilent Technologies gelieferten Kabel, um WARNUNG eine einwandfreie Funktion und Übereinstimmung mit den gesetzlichen Vorschriften...

  • Page 130: Sicherheitssymbole

    Sicherheit Sicherheitshinweise Sicherheitssymbole Tabelle 13 Sicherheitssymbole am Gerät und in diesem Handbuch Symbol Beschreibung Das Gerät ist mit diesem Symbol gekennzeichnet, wenn der Bediener das Benutzerhandbuch lesen sollte, um Verletzungen zu vermeiden und das Gerät vor Schäden zu schützen. Chemische und biologische Sicherheit Beachten Sie beim Arbeiten mit Lösungsmitteln die entsprechenden WARNUNG Sicherheitsmaßnahmen (z.
  • Page 131 Originallösungsmittelbehältern. Da durch Lecks, verschüttetes Lösungsmittel und Probenverluste Aerosole entstehen können, beachten Sie die entsprechenden Sicherheitsmaßnahmen. Die Abdeckungen des Agilent 3100 OFFGEL Fractionator sind nicht als biologisch wirksame Dichtung für die Rückhaltung von Aerosolen und Flüssigkeiten ausgeführt. Handhaben Sie Körperflüssigkeiten mit Vorsicht, da sie Krankheiten übertragen können.
  • Page 132 Sicherheit Sicherheitshinweise OFFGEL Benutzerhandbuch...

  • Page 133: Sécurité

    Fractionneur Agilent 3100 OFFGEL Manuel de l’utilisateur Sécurité Consignes de sécurité Ce chapitre contient des informations de sécurité importantes concernant le Fractionneur Agilent 3100 OFFGEL. Agilent Technologies...

  • Page 134: Consignes De Sécurité

    à la concep- tion, à la fabrication et à l’utilisation normale de l’instrument. Agilent Technologies ne saurait être tenu pour responsable en cas de non observation de ces consignes par le client.

  • Page 135: Cordons Secteur

    Ne jamais faire fonctionner un instrument si la borne de terre de l'alimentation DANGER secteur n'est pas effectivement reliée à la terre. Ne jamais utiliser un cordon secteur différent de celui spécifiquement fourni par Agilent Technologies pour la région d'utilisation. Afin d'assurer un fonctionnement correct et conforme à la réglementation sur la DANGER compatibilité...

  • Page 136: Symboles De Sécurité

    Sécurité Consignes de sécurité Utiliser exclusivement des pièces d'origine Agilent et ne pas effectuer de modification non autorisée de l'instrument. Symboles de sécurité Table 13 Symboles de sécurité utilisés sur les instruments et dans les manuels Symbole Description L’appareil est marqué de ce symbole lorsque l’utilisateur doit consulter le Manuel de l’utilisateur afin de se prémunir contre les risques de blessure corporelle et de protéger l’appareil contre tout dommage.
  • Page 137 Sécurité Consignes de sécurité Si des échantillons pathogènes, toxiques ou radioactifs doivent être utilisés dans cet DANGER instrument, il est de la responsabilité de l’utilisateur de s’assurer que toutes les règles de sécurité, directives, précautions et pratiques sont respectées en conséquence.
  • Page 138 Sécurité Consignes de sécurité Manuel de l’utilisateur OFFGEL...

  • Page 139: Sicurezza

    Frazionatore OFFGEL Agilent 3100 Manuale per l'utente Sicurezza Informazioni per la sicurezza Questo capitolo contiene informazioni importanti per la sicurezza relative al sistema Frazionatore OFFGEL Agilent 3100. Agilent Technologies...

  • Page 140: Informazioni Per La Sicurezza

    Agilent Technologies non può in nessun modo essere ritenuta responsabile del mancato rispetto delle normative in vigore da parte dell'utilizzatore. Un segnale di allarme richiama l'attenzione su una situazione che potrebbe causare ATTENZIONE danni alle persone o alla strumentazione.

  • Page 141: Cavo Di Alimentazione

    Technologies per l'uso nel proprio paese. Per un funzionamento ottimale e per rispettare le normative di sicurezza EMC, è ATTENZIONE importante utilizzare solo i cavi di alimentazione forniti da Agilent Technologies. Funzionamento Prima di alimentare il sistema, seguire attentamente le istruzioni per l'installazione affinché...

  • Page 142: Simboli Di Sicurezza

    Sicurezza Informazioni per la sicurezza Simboli di sicurezza Tabella 13 Simboli di sicurezza utilizzati per gli strumenti e per la documentazione tecnica Simbolo Descrizione L'apparecchiatura è contrassegnata da questo simbolo quando l'utente deve consultare il Manuale per l'utente per evitare danni all'operatore e proteggere gli strumenti da eventuali danni.
  • Page 143 Sicurezza Informazioni per la sicurezza Se si intende utilizzare campioni tossici o radioattivi sullo strumento, è ATTENZIONE responsabilità dell'utente garantire che tutte le norme di sicurezza in vigore, linee guida, precauzioni e pratiche vengano rispettate adeguatamente. Questo anche in caso di manipolazione di organismi geneticamente modificati. Informarsi presso il responsabile della sicurezza del laboratorio sul livello di protezione richiesta per l'applicazione in uso e sui procedimenti di decontaminazione e sterilizzazione da seguire, in caso i fluidi fuoriescano dai contenitori.
  • Page 144 Sicurezza Informazioni per la sicurezza Manuale per l’utente del frazionatore OFFGEL...

  • Page 145: Seguridad

    Fraccionador 3100 OFFGEL de Agilent Manual de usuario Seguridad Información de seguridad En este capítulo encontrará información de seguridad importante relativa al Fraccionador 3100 OFFGEL de Agilent. Agilent Technologies...

  • Page 146: Información De Seguridad

    Agilent Technologies no se responsabiliza del incumplimiento por parte del usuario de estas normas. Un aviso advierte sobre una situación que podría derivar en lesiones personales AVISO o daños al equipo.

  • Page 147: Operación

    No utilice nunca un cable de alimentación que no haya sido suministrado por Agilent Technologies y diseñado para su zona geográfica. No utilice nunca cables que no hayan sido suministrados por Agilent Technologies AVISO para asegurar un correcto funcionamiento así como el cumplimiento de las normativas de seguridad o de compatibilidad electromagnética.

  • Page 148: Símbolos De Seguridad

    Seguridad Información de seguridad Símbolos de seguridad Tabla 13 Símbolos de seguridad empleados en los instrumentos y en los manuales Símbolo Descripción El instrumento aparece marcado con este símbolo para indicar que el usuario debe consultar el Manual de usuario con el fin de evitar posibles lesiones al operador y para proteger el equipo contra posibles daños.
  • Page 149 Seguridad Información de seguridad Si se tiene previsto utilizar muestras patógenas, tóxicas o radioactivas en este AVISO instrumento, es responsabilidad del usuario asegurar que se observen adecuadamente todos los reglamentos, directrices, precauciones y prácticas de seguridad. Esto también incluye la manipulación de organismos modificados genéticamente.
  • Page 150 Seguridad Información de seguridad Manual de usuario de OFFGEL...

  • Page 151: Appendix

    Agilent 3100 OFFGEL Fractionator User Manual Appendix Non-condensing Conditions Ordering Information This chapter contains details on non-condensing conditions as well as ordering information for consumables and spare parts. Agilent Technologies...

  • Page 152: Non-Condensing Conditions

    Appendix Non-condensing Conditions Non-condensing Conditions The Agilent 3100 OFFGEL Fractionator is specified to be operated only under non-condensing conditions and with a cooling platform temperature of maximum 10 °C below ambient temperature. For example, assuming a typically experimental setup of 20 °C on the cooling platform and an ambient temperature of 25 °C, the humidity must be below...

  • Page 153: Ordering Information

    Appendix Ordering Information Ordering Information List of Consumables Table 14 Agilent 3100 OFFGEL Fractionator consumable kits Product Order Number Product Description 3100 OFFGEL, 3-10, Low Res Kit 5188-6425 includes frames (12 well), IPG-strips pH 3-10 (13 cm), cover seals and fractionation reagents for 12 samples.

  • Page 154: List Of Spare Parts

    Appendix Ordering Information List of Spare Parts Table 15 Agilent 3100 OFFGEL Fractionator spare parts Product Order Number Product Description Tray Set G3100-60007 includes 4 replacement trays Electrode Assy G3100-60002 includes 1 tray, 1 fixed electrode and 1 movable electrode...
  • Page 155 Index Index Symbols Consignes de sécurité, consumable kits, 19, 安全说明 , gases inflamables, control, general functions, control module, 17, control PC, 安全性について , cooling platform, hardware diagnosis, accessories, cleaning, humidity, 25, add instrument, temperature, anode, cover seals, anode connector, csv file, 58, Informazioni per la sicurezza, cleaning, in-gel mode, 14, 16, 66,...
  • Page 156 Index protein test sample, 19, 105, 107, tray, 19, 66, protocol file, cleaning, lock, connection, 28, 34, 63, 78, preparing, firmware update tool installation, report screen, set, firmware update tool setup, result, 58, troubleshooting line frequency, 22, RF transmitters, application, line voltage, 22, riesgo para la seguridad, instrument,...
  • Page 158 • Fractionation principles • Installation and configuration • Using the browser interface • Maintenance and Troubleshooting • Spare parts and consumables • Safety and related information © Agilent Technologies 2010 Printed in Germany 04/2010 *G3100-90001* *G3100-90001* G3100-90001 Agilent Technologies...

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