Troubleshooting The Electrodes; Checking Fixed And Movable Electrodes - Agilent Technologies 3100 OFFGEL Fractionator User Manual

7 Troubleshooting

Troubleshooting the Electrodes

Troubleshooting the Electrodes
Electrodes can be used multiple times. They are designed to be robust but they
must still be regarded as consumable. They might suffer from permanent
coating of the surface and from residues formed by arcing events during
fractionations.
Coatings of the surface may derive from substances in sample buffers that are
not hold back by the gel system and can modify the electrodes. Arcing during
fractionation might happen if an insufficient amount of liquid is applied to
pads in the assembly of the experimental setup or if these pads dry out during
the fractionation.

Checking Fixed and Movable Electrodes

1 Make sure your two electrode sets and trays are completely dry and clean.
2 For movable electrodes measure the resistance from the tip of the electrode
3 For movable electrodes measure the resistance from the tip of the electrode
The resistance for each of this measurement should have a value below
100 kOhm. In case the measured resistance is higher, exchange the respective
electrode. See
100
(8 individual pins) which contact to the pad to the tip of the anode
connector electrode (one pins).
(8 individual pins) which contact to the gel strip at the cathode side to the
metal spring of the movable electrode (8 individual metal springs).
page 156 for product numbers for spare parts.
OFFGEL User Manual