Looking At Run Parameters; Example 1. Typical Results Of The Protein Test Sample - Agilent Technologies 3100 OFFGEL Fractionator User Manual

Troubleshooting 7
Troubleshooting the Application

Looking at Run Parameters

The results of all run parameters can be exported as a .csv file and thus can be
visualized with Microsoft Excel. Agilent Technologies has designed the Agilent
3100 OFFGEL fractionator plotter which is a Excel add-on and facilitates the
analysis of the run parameters (see "How to Display Exported Results" on
page 58). The following examples show typical results for good runs as well as
some impaired results where the root cause of the problem is known.

Example 1. Typical Results of the Protein Test Sample

The fractionation of the protein test sample is a way to check that the Agilent
3100 OFFGEL Fractionator in combination with the consumables kit is
working in your hands, and functions as a system checkout test. You find
detailed information on how to run the protein test sample in the kit manual.
Figure 45 shows a typical result for the electrical run parameters of the
protein test sample using the 3100 OFFGEL Low Res Kit, pH 3-10. The method
used for this fractionation was the default method OG12PR00, which has a
current limit of 50 µA, a voltage limit of 8000 V, a power limit of 200 mW and a
volt hour limit of 20 kVh. With this setup the fractionation is running in
current limiting mode. Therefore, the current signal in Figure 45 is stable and
at 50 µA.
Please note that latest OFFGEL methods do have a voltage limit of 4500 V. This is reduce
N O T E
the risk of arcing and damage to the tray in case of dried out electrode pads.
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