Checking High Voltage, Electrodes And Electrode Connectors - Agilent Technologies 3100 OFFGEL Fractionator User Manual

Checking High Voltage, Electrodes and Electrode Connectors

1 Make sure your two electrode sets and trays are completely dry and clean.
2 Assemble both trays with the electrodes (without frames) and insert them
3 Load the DIAGNOSE method to both trays and press Start on both trays to
4 Check that the actual voltage values reach approximately 800 V after one
If this is not the case, check once again that the tray and the electrodes are
completely dry and clean. Inspect the cathode connector for contamination
and clean it if necessary. Make sure that no sparking occurs. If you cannot
solve this problem call Agilent Technologies for further help. Otherwise,
proceed with
5 Press Stop to stop both runs.
6 Open the top cover and pipette 4 ml deionized water (18 MOhm/m) into
7 Close the top cover.
Make sure the water is evenly distributed and the electrode pins are in contact with the
N O T E
water. However, avoid spillage of water across the electrodes into neighboring lanes and
the electrode connectors. This might result in sparking.
8 Press Start on both trays to start the DIAGNOSE method again.
9 Look at both tray status screens. You should see a regular current pattern
OFFGEL User Manual
to the instrument. Attach the movable electrodes at the most right position.
start the runs.
minute and that the currents of all lanes are < 1 µA.
step 5.
lanes A, C, E and G on each tray.
as shown in Figure 43.
Troubleshooting
Troubleshooting the Instrument
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