Preparing A Tray For The Offgel Mode; Preparing A Tray For The Standard Ipg Ief Mode - Agilent Technologies 3100 OFFGEL Fractionator User Manual

5 Operation
Performing a Fractionation
Please note that latest OFFGEL methods do have a voltage limit of 4500 V. This voltage limit
N O T E
reduces the risk of arcing and damage to the tray in case of dried out electrode pads.

Preparing a Tray for the OFFGEL Mode

To prepare a tray for the OFFGEL mode, refer to the Kit Manual (5969-1582)
for detailed instructions. The manual for OFFGEL fractionation is included in
every consumable kit (see

Preparing a Tray for the Standard IPG IEF Mode

You can use any IPG-strip for fractionations in the standard IPG IEF mode
(in-gel mode). Follow the recommendations of the IPG-strip supplier about
rehydration loading of the sample to the IPG-strip. The tray of the Agilent 3100
OFFGEL Fractionator system can be used as a rehydration tray.
The following workflow uses an IPG-strip already loaded with sample and
describes an example of the tray preparation for one IPG strip.
1 Place the IPG-strip in a new tray lane with the gel side up. The low pH side
2 Take two electrode pads with tweezers, wet them with rehydration buffer,
3 Attach the electrodes to the tray exactly as described in the OFFGEL Kit
4 Place the tray into the instrument.
5 Cover the complete tray lane with mineral oil using 1.5 ml mineral oil per
66
(Anode), marked with a "+", must be at the left, where the fixed electrode
will be attached.
and place one at each end of the IPG-strip so that it connects to the swelled
gel.
Manual (5969-1582). If necessary, reposition the IPG-strip with the
electrode pads so that the fixed electrode connects to the left electrode pad.
IPG-strip.
Table 1 on page 19).
OFFGEL User Manual