Show Basic Quantitative Data; Chromatograms - AB Sciex QTRAP 4500 LC/MS/MS system System User's Manual

Show Basic Quantitative Data

Prerequisite Procedure
• Open Data Files on page 77
1. In the Peak List tab, right-click and select Show Peaks in Graph.
Peaks are shown in two colors.
2. To change the peak finding algorithm settings, right-click and then select either
Analyst Classic Parameters or Intelliquan Parameters, which ever is active.
3. (Optional) To remove the colored peaks, right-click in the Peak List tab and then
clear Show Peaks in Graph.

Chromatograms

Table 10-4 Chromatograms
Types of Chromatograms Purpose
TIC (Total Ion Chromatogram)
XIC (Extracted Ion
Chromatogram)
BPC (Base Peak
Chromatogram)
TWC (Total Wavelength
Chromatogram)
XWC (Extracted Wavelength
Chromatogram)
DAD (Diode Array Detector)
System User Guide
RUO-IDV-05-0264-A
A chromatographic display generated by plotting the intensity
of all ions in a scan against time or scan number.
When a data file is opened, it is preset to appear as a TIC. If
the experiment contains only one scan, it is shown as a
spectrum. For more information about using the available
icons, refer to Table 10-8.
If the MCA check box is selected during acquisition of the data
file, then the data file opens to the mass spectrum. If the MCA
check box is not selected, then the data file opens with the
TIC.
An ion chromatogram created by taking intensity values at a
single, discrete mass value, or a mass range, from a series of
mass spectral scans. It indicates the behavior of a given mass,
or mass range, as a function of time.
A chromatographic plot that shows the intensity of the most
intense ion within a scan versus time or scan number.
A chromatographic display created by summing all of the
absorbance values in the acquired wavelength range and then
plotting the values against time. It consists of the summed
absorbances of all ions in a scan plotted against time in a
chromatographic pane.
A subset of TWC. An XWC shows the absorbance for a single
wavelength or the sum of the absorbance for a range of
wavelengths.
A UV detector that monitors the absorption spectrum of eluting
compounds at one or more wavelengths.
Operating Instructions — Analyze and Process Data
4500 Series of Instruments
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