Appendix B Calibration Ions And Solutions - AB Sciex QTRAP 4500 LC/MS/MS system System User's Manual

Calibration Ions and Solutions
Table B-1 Tuning Frequency
Calibration
Scan Type Frequency
Q1 and Q3 3 to 6 months
LIT Every 2 weeks;
as required
Table B-2 Suggested Tuning Solutions for AB SCIEX Triple Quad™ 4500 System
System
AB SCIEX Triple Quad 4500 system
Table B-3 Suggested Tuning Solutions for QTRAP
Instrument
QTRAP 4500 LC/MS/MS
system
Table B-4 Q1 and Q3 PPG Positive Ion Scans
Instrument
AB SCIEX Triple Quad 4500
system
QTRAP 4500 LC/MS/MS
system
Table B-5 Q1 and Q3 PPG Negative Ion Scans
Instrument
AB SCIEX Triple Quad
4500 system
QTRAP 4500 LC/MS/MS
system
a Table B-6 Masses and Polarity for the QTRAP 4500 LC/MS/MS System (Agilent)
Instrument/ Masses
Polarity
LIT Positive 118.087
LIT Negative 112.985
System User Guide
RUO-IDV-05-0264-A
Resolution Optimization
Manual/ Frequency
Automated
Both 3 to 6 months
Both 3 to 6 months
Positive
–6
2 × 10 M PPG (1:50)
Q1 and Q3
Positive
–6
2 × 10 M PPG (1:50) 3 × 10
Masses
59.0 175.1 500.3 616.5 906.7
59.0 175.1 500.3 616.5 906.7
Masses
45.0 411.2 585.4 933.6
45.0 411.2 585.4 933.6
322.049 622.030 922.010
431.982 601.978 1033.988 1633.949 –
Manual/Automated
Both
Automated only
Negative
–4
3 × 10
®
4500 LC/MS/MS System
Negative Positive and
Negative
–4
NEG PPG 1:100 Agilent mix
1254.9 1545.1 1952.4
1254.9 1545.1 1952.4
1223.8 1572.1 1863.3 1979.3
1223.8 1572.1 1863.3 1979.3
1521.972 –
B
NEG PPG
LIT
–
–
4500 Series of Instruments
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