Generate Twcs; Adjust The Threshold - AB Sciex QTRAP 4500 LC/MS/MS system System User's Manual

Qtrap 4500 series
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Generate TWCs

A TWC shows total absorbance (mAU) on the y-axis plotted against time on the x-axis. For more
information about using the available icons, refer to
Prerequisite Procedure
Open Data Files on page
Click Explore > Show > Show DAD TWC.
The TWC opens in a pane below the DAD spectrum.
Tip!
click Show DAD TWC.

Adjust the Threshold

The threshold is an invisible line drawn parallel to the x-axis of a graph that sets a limit below
which the software will not include peaks in a spectrum. The line has a handle, represented by a
blue triangle to the left of the y-axis. Click the blue triangle to view a dotted line that represents
the threshold. The threshold can be raised or lowered, but changing the threshold value does not
change the data. The software does not label any peaks in the region that lies below the
threshold.
Prerequisite Procedure
Open Data Files on page 77
Do one of the following:
To raise the threshold, drag the blue triangle up the y-axis. To lower the
threshold, drag the blue triangle down.
Click Explore > Set Threshold. In the Threshold Options dialog that opens,
type the threshold value.
Click Explore > Threshold.
The graph updates to show the new threshold. Peak labeling and the peak list are
also updated.
Tip!
threshold handle.
Table 10-6 Right-Click Menu for Chromatogram Panes
Menu
List Data
Show Spectrum
System User Guide
RUO-IDV-05-0264-A
77. Open a data file that contains a DAD spectrum.
Right-click inside the pane containing the DAD spectrum and then
To view the current threshold value, move the pointer over the
Function
Lists the data points and integrates the peaks found in
chromatograms.
Generates a new pane.
Operating Instructions — Analyze and Process Data
Table
10-8.
4500 Series of Instruments
87 of 138

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