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Operating Instructions — Analyze and Process Quantitative Data
versus the ratio of the standard peak height or area to the internal standard peak height or area is
plotted. The area or height ratio of a sample is then applied to this curve to find the concentration
of the sample, as shown in the Results Table. A regression equation is generated by this
calibration curve according to the regression that was specified. The regression equation is used
to calculate the concentration of the unknown samples.
View Calibration Curves
The user can view the calibration curve and change the regression options in an open Results
Table. If two or more Results Tables are open, the calibration curves can be overlaid. To overlay
curves, the method used to create the tables must be the same.
Plot a calibration curve to see the curve used for regression. The Calculated Concentration
field in the Results Table reflects any changes resulting from the fit of the curve to the points of
the standard.
Note: This option is available only when a Results Table is open in the workspace.
1. With a Results Table open, click Tools > Calibration > Pane.
The Calibration Curve pane containing the calibration curve opens.
2. If there is more than one analyte, then use the following steps to view the calibration
curve for another analyte:
i.
In the Analyte list, select an analyte.
ii. If required, in the next list, select Area or Height.
3. To change the regression options for the calibration curve, do the following:
i.
Click Regression.
Figure 11-11 Regression Options Dialog
ii. In the Fit list, select Linear.
iii. In the Weight list, select 1 / x.
iv. Click OK.
The calibration curve opens. The user can review individual peaks on the curve or
exclude points from the curve to produce a better curve.
4. If required, repeat these steps to create a more appropriate curve.
5. To save the changes, click Accept.
4500 Series of Instruments
108 of 138
System User Guide
RUO-IDV-05-0264-A
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