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Operating Instructions — Tune and Calibrate
Run the Verify instrument performance option weekly or after the mass spectrometer is cleaned
to confirm that the system is working properly. In general, for triple quadrupole systems,
calibration and resolution is satisfactory for three to six months unless the system loses vacuum.
For linear ion trap (LIT) systems, the resolution should also be satisfactory for three to six months
but the system should be calibrated approximately monthly. If the system loses vacuum, then
check the calibration and resolution before using the system. For more information about tuning
and calibration, refer to the Advanced User Guide and the Manual Tuning Tutorial.
Tip!
Perform maintenance tasks regularly to make sure that the mass spectrometer is
performing optimally. Refer to
Required Materials
• Tuning solutions that are supplied in the Standards Chemical Kit shipped with the system. If
needed, a new kit can be ordered from AB SCIEX.
• 5 mL, 1 mL, and 250 µL serial gas-tight syringes.
• red PEEK sample tubing.
Prerequisites
• A printer is configured.
• The spray is stable and the correct tuning solution is being used.
Optimize the Instrument
The following procedure describes how to verify the performance of the instrument. For more
information about using the other instrument performance options, refer to the Help.
1. In the navigation bar, under Tune and Calibrate, double-click Manual Tuning.
2. Run a calibration method, and confirm that the TIC is stable and that the peaks of
interest are present in the spectrum.
3. In the navigation bar, under Tune and Calibrate, double-click Instrument
Optimization.
The Instrument Optimization dialog opens.
The instrument name and the current operating mode configured in the active
hardware profile is shown at the bottom of the dialog.
4. Click Verify instrument performance.
5. Click Next.
6. Click Approved Tuning.
7. Click Next.
System User Guide
RUO-IDV-05-0264-A
Table 14-1 on page
129.
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4500 Series of Instruments
49 of 138
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